Pseudomonas effector AvrB is a glycosyltransferase that rhamnosylates plant guardee protein RIN4

Peng, Wei; Garcia, Nalleli; Servage, Kelly A.; Kohler, Jennifer J.; Ready, Joseph M.; Tomchick, Diana R.; Fernandez, Jessie; Orth, Kim

doi:10.1126/sciadv.add5108

Title: Pseudomonas effector AvrB is a glycosyltransferase that rhamnosylates plant guardee protein RIN4

Journal Article · 14 February 2024 · Science Advances

DOI: https://doi.org/10.1126/sciadv.add5108 · OSTI ID:2470049

^[1]; Garcia, Nalleli ^[2];

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^[2];

^[1]

University of Texas Southwestern Medical Center, Dallas, TX (United States)
University of Florida, Gainesville, FL (United States)

The plant pathogen Pseudomonas syringae encodes a type III secretion system avirulence effector protein, AvrB, that induces a form of programmed cell death called the hypersensitive response in plants as a defense mechanism against systemic infection. Despite the well-documented catalytic activities observed in other Fido (Fic, Doc, and AvrB) proteins, the enzymatic activity and target substrates of AvrB have remained elusive. Here, we show that AvrB is an unprecedented glycosyltransferase that transfers rhamnose from UDP-rhamnose to a threonine residue of the Arabidopsis guardee protein RIN4. We report structures of various enzymatic states of the AvrB-catalyzed rhamnosylation reaction of RIN4, which reveal the structural and mechanistic basis for rhamnosylation by a Fido protein. Collectively, our results uncover an unexpected reaction performed by a prototypical member of the Fido superfamily while providing important insights into the plant hypersensitive response pathway and foreshadowing more diverse chemistry used by Fido proteins and their substrates.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Biological and Environmental Research (BER); Welch Foundation

Grant/Contract Number:: AC02-05CH11231; AC02-06CH11357

OSTI ID:: 2470049

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 7 Vol. 10; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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