A novel conjugative transposon carrying an autonomously amplified plasmid

Vineis, Joseph H.; Reznikoff, William S.; Antonopoulos, Dionysios A.; Koval, Jason; Chang, Eugene; Fallon, Bailey R.; Paul, Blair G.; Morrison, Hilary G.; Sogin, Mitchell L.

doi:10.1128/mbio.02787-23

Title: A novel conjugative transposon carrying an autonomously amplified plasmid

Journal Article · 14 February 2024 · mBio (Online)

DOI: https://doi.org/10.1128/mbio.02787-23 · OSTI ID:2469987

^[1]; Reznikoff, William S. ^[1]; Antonopoulos, Dionysios A. ^[2]; Koval, Jason ^[2]; Chang, Eugene ^[3]; Fallon, Bailey R. ^[1]; Paul, Blair G. ^[1];

^[1];

^[1]

Marine Biological Laboratory, Woods Hole, MA (United States)
Argonne National Lab., Idaho Falls, ID (United States)
Univ. of Chicago, IL (United States)

Tetracyclines serve as broad-spectrum antibiotics to treat bacterial infections. The discovery of new tetracycline resistance genes has led to new questions about the underlying mechanisms of resistance, gene transfer, and their relevance to human health. We tracked changes in the abundance of a 55-kbp conjugative transposon (CTn214) carrying tetQ, a tetracycline resistance gene, within a Bacteroides fragilis metagenome-assembled genome derived from shotgun sequencing of microbial DNA extracted from the ileal pouch of a patient with ulcerative colitis. The mapping of metagenomic reads to CTn214 revealed the multi-copy nature of a 17,044-nt region containing tetQ in samples collected during inflammation and uninflamed visits. B. fragilis cultivars isolated from the same patient during periods of inflammation harbored CTn214 integrated into the chromosome or both a circular, multi-copy, extrachromosomal region of the CTn214 containing tetQ and the corresponding integrated form. The tetracycline-dependent mechanism for the transmission of CTn214 is nearly identical to a common conjugative transposon found in the genome of B. fragilis (CTnDOT), but the autonomously amplified nature of a circular 17,044-nt region of CTn214 that codes for tetQ and the integration of the same sequence in the linear chromosome within the same cell is a novel observation. Genome and transcriptome sequencing of B. fragilis cultivars grown under different concentrations of tetracycline and ciprofloxacin indicates that tetQ in strains containing the circular form remains actively expressed regardless of treatment, while the expression of tetQ in strains containing the linear form increases only in the presence of tetracycline.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 2469987

Journal Information:: mBio (Online), Journal Name: mBio (Online) Journal Issue: 2 Vol. 15; ISSN 2150-7511

Publisher:: American Society for Microbiology (ASM)Copyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Bacteroides fragilis
CTnDOT
antibiotic resistance
conjugal transposon
host–microbe interactions
microbial evolution

Title: A novel conjugative transposon carrying an autonomously amplified plasmid

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