Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells

Tyson, Jonathan; Hu, Kevin; Zheng, Shuai; Kidd, Phylicia; Dadina, Neville; Chu, Ling; Toomre, Derek; Bewersdorf, Joerg; Schepartz, Alanna

doi:10.1021/acscentsci.1c00670

Title: Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells

Journal Article · 23 July 2021 · ACS Central Science

DOI: https://doi.org/10.1021/acscentsci.1c00670 · OSTI ID:2469690

Tyson, Jonathan ^[1]; Hu, Kevin ^[2]; Zheng, Shuai ^[3]; Kidd, Phylicia ^[2]; Dadina, Neville ^[3]; Chu, Ling ^[2]; Toomre, Derek ^[2]; Bewersdorf, Joerg ^[2];

^[1]

Univ. of California, Berkeley, CA (United States); Yale Univ., New Haven, CT (United States)
Yale Univ., New Haven, CT (United States)
Univ. of California, Berkeley, CA (United States)

New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Molecular Foundry

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2469690

Journal Information:: ACS Central Science, Journal Name: ACS Central Science Journal Issue: 8 Vol. 7; ISSN 2374-7943

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Absorption
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