A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function

Crooks, Casey; Bechle, Nathan J.; St John, Franz J.

doi:10.3389/fmolb.2021.714238

Title: A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function

Journal Article · 07 September 2021 · Frontiers in Molecular Biosciences

DOI: https://doi.org/10.3389/fmolb.2021.714238 · OSTI ID:2469507

Crooks, Casey ^[1]; Bechle, Nathan J. ^[1]; St John, Franz J. ^[1]

USDA Forest Service, Madison, WI (United States)

The Acetivibrioclariflavus (basonym: Clostridium clariflavum) glycoside hydrolase family 30 cellulosomal protein encoded by the Clocl_1795 gene was highly represented during growth on cellulosic substrates. In this report, the recombinantly expressed protein has been characterized and shown to be a non-reducing terminal (NRT)-specific xylobiohydrolase (AcXbh30A). Biochemical function, optimal biophysical parameters, and phylogeny were investigated. The findings indicate that AcXbh30A strictly cleaves xylobiose from the NRT up until an α-1,2-linked glucuronic acid (GA)-decorated xylose if the number of xyloses is even or otherwise a single xylose will remain resulting in a penultimate GA-substituted xylose. Unlike recently reported xylobiohydrolases, AcXbh30A has no other detectable hydrolysis products under our optimized reaction conditions. Sequence analysis indicates that AcXbh30A represents a new GH30 subfamily. This new xylobiohydrolase may be useful for commercial production of industrial quantities of xylobiose.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 2469507

Journal Information:: Frontiers in Molecular Biosciences, Journal Name: Frontiers in Molecular Biosciences Vol. 8; ISSN 2296-889X

Publisher:: Frontiers Media S.A.Copyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
AcXbh30A
Acetivibrio clariflavus
Hungateiclostridium clariflavum
glucuronoxylan
glycoside hydrolase
glycoside hydrolase family 30
xylobiohydrolase
xylobiose

Title: A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function

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