Hyperspectral acquisition with ScanImage at the single pixel level: application to time domain coherent Raman imaging

Journal Article · 11 October 2024 · Optics Express

DOI: https://doi.org/10.1364/OE.533880 · OSTI ID:2466250

Metais, Samuel; Suresh, Sisira; Diniz, Paulo; Vourdaki, Chrysa; Martin, Ines; Shivkumar, Siddarth ; Bartels, Randy ; Forget, Nicolas; Rigneault, Hervé

We present a comprehensive strategy and its practical implementation using the commercial ScanImage software platform to perform hyperspectral point scanning microscopy when a fast time-dependent signal varies at each pixel level. In the proposed acquisition scheme, the scan along the X-axis is slowed down while the data acquisition is maintained at a high pace to enable the rapid acquisition of the time-dependent signal at each pixel level. The ScanImage generated raw 2D images have a very asymmetric aspect ratio between X and Y, the X axis encoding both for space and time acquisition. The results are X-axis macro-pixel where the associated time-dependent signal is sampled to provide hyperspectral information. We exemplified the proposed hyperspectral scheme in the context of time-domain coherent Raman imaging, where a pump pulse impulsively excites molecular vibrations that are subsequently probed by a time-delayed probe pulse. In this case, the time-dependent signal is a fast acousto-optics delay line that can scan a delay of 4.5ps in 25 μ s at each pixel level. With this acquisition scheme, we demonstrate ultra-fast hyperspectral vibrational imaging in the low frequency range [10 cm ⁻¹ , 150 cm ⁻¹ ] over a 500 μm field of view (64 x 64 pixels) in 130ms (∼ 7.5 frames/s). The proposed acquisition scheme can be readily extended to other applications requiring the acquisition of a fast-evolving signal at each pixel level.

• Sponsoring Organization: USDOE
• OSTI ID: 2466250
• Journal Information: Optics Express, Journal Name: Optics Express Journal Issue: 22 Vol. 32; ISSN 1094-4087; ISSN OPEXFF
• Publisher: Optical Society of AmericaCopyright Statement
• Country of Publication: United States
• Language: English