Cryo-EM sample preparation for high-resolution structure studies

Wang, Liguo; Zimanyi, Christina M.

doi:10.1107/s2053230x24002553

Title: Cryo-EM sample preparation for high-resolution structure studies

Journal Article · 26 March 2024 · Acta Crystallographica. Section F, Structural Biology Communications

DOI: https://doi.org/10.1107/s2053230x24002553 · OSTI ID:2448257

Wang, Liguo ^[1];

^[2]

Brookhaven National Laboratory (BNL), Upton, NY (United States)
New York Structural Biology Center (NYSBC), NY (United States). Simons Electron Microscopy Center (SEMC)

High-resolution structures of biomolecules can be obtained using single-particle cryo-electron microscopy (SPA cryo-EM), and the rapidly growing number of structures solved by this method is encouraging more researchers to utilize this technique. As with other structural biology methods, sample preparation for an SPA cryo-EM data collection requires some expertise and an understanding of the strengths and limitations of the technique in order to make sensible decisions in the sample-preparation process. Here, in this article, common strategies and pitfalls are described and practical advice is given to increase the chances of success when starting an SPA cryo-EM project.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); Simons Foundation

Grant/Contract Number:: SC0012704

OSTI ID:: 2448257

Report Number(s):: BNL--226166-2024-JAAM

Journal Information:: Acta Crystallographica. Section F, Structural Biology Communications, Journal Name: Acta Crystallographica. Section F, Structural Biology Communications Journal Issue: 4 Vol. 80; ISSN 2053-230X

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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