A High-Throughput Screening Platform for Engineering Poly(ethylene Terephthalate) Hydrolases

Groseclose, Thomas M.; Kober, Erin A.; Clark, Matilda; Moore, Benjamin; Banerjee, Shounak; Bemmer, Victoria; Beckham, Gregg T.; Pickford, Andrew R.; Dale, Taraka T.; Nguyen, Hau B.

doi:10.1021/acscatal.4c04321

Title: A High-Throughput Screening Platform for Engineering Poly(ethylene Terephthalate) Hydrolases

Journal Article · 17 September 2024 · ACS Catalysis

DOI: https://doi.org/10.1021/acscatal.4c04321 · OSTI ID:2446542

^[1];

^[1]; Clark, Matilda ^[2]; Moore, Benjamin ^[2];

^[1]; Bemmer, Victoria ^[2];

^[3];

^[2]; Dale, Taraka T. ^[1];

^[1]

Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States, BOTTLE Consortium, Golden, Colorado 80401, United States
BOTTLE Consortium, Golden, Colorado 80401, United States, Centre for Enzyme Innovation, School of the Environmental and Life Sciences, University of Portsmouth, Portsmouth, PO1 2DT, U.K.
BOTTLE Consortium, Golden, Colorado 80401, United States, Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, Colorado 80401, United States

Not Available

View Journal Article

Cite

Export

Save

Sponsoring Organization:: USDOE

OSTI ID:: 2446542

Journal Information:: ACS Catalysis, Journal Name: ACS Catalysis Journal Issue: 19 Vol. 14; ISSN 2155-5435

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

References (54)

A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate Then, Johannes; Wei, Ren; Oeser, Thorsten FEBS Open Bio, Vol. 6, Issue 5 https://doi.org/10.1002/2211-5463.12053	journal	April 2016
Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures Wei, Ren; Breite, Daniel; Song, Chen Advanced Science, Vol. 6, Issue 14 https://doi.org/10.1002/advs.201900491	journal	May 2019
Complete Depolymerization of PET Wastes by an Evolved PET Hydrolase from Directed Evolution Shi, Lixia; Liu, Pi; Tan, Zijian Angewandte Chemie International Edition, Vol. 62, Issue 14 https://doi.org/10.1002/anie.202218390	journal	February 2023
A high‐throughput assay for enzymatic polyester hydrolysis activity by fluorimetric detection Wei, Ren; Oeser, Thorsten; Billig, Susan Biotechnology Journal, Vol. 7, Issue 12 https://doi.org/10.1002/biot.201200119	journal	June 2012
Ca ²⁺ and Mg ²⁺ binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases from Thermobifida fusca Then, Johannes; Wei, Ren; Oeser, Thorsten Biotechnology Journal, Vol. 10, Issue 4 https://doi.org/10.1002/biot.201400620	journal	January 2015
Engineered bacterial polyester hydrolases efficiently degrade polyethylene terephthalate due to relieved product inhibition: Engineered Polyester Hydrolases Wei, Ren; Oeser, Thorsten; Schmidt, Juliane Biotechnology and Bioengineering, Vol. 113, Issue 8 https://doi.org/10.1002/bit.25941	journal	February 2016
Low Carbon Footprint Recycling of Post‐Consumer PET Plastic with a Metagenomic Polyester Hydrolase Sonnendecker, Christian; Oeser, Juliane; Richter, P. Konstantin ChemSusChem, Vol. 15, Issue 9 https://doi.org/10.1002/cssc.202101062	journal	February 2022
Concentration‐Dependent Inhibition of Mesophilic PETases on Poly(ethylene terephthalate) Can Be Eliminated by Enzyme Engineering Avilan, Luisana; Lichtenstein, Bruce R.; König, Gerhard ChemSusChem, Vol. 16, Issue 8 https://doi.org/10.1002/cssc.202202277	journal	March 2023
Clustal Omega for making accurate alignments of many protein sequences: Clustal Omega for Many Protein Sequences Sievers, Fabian; Higgins, Desmond G. Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3290	journal	October 2017
On the temperature dependence of enzymatic degradation of poly(ethylene terephthalate) Akram, Ekram; Cao, Yufei; Xing, Hao Chinese Journal of Catalysis, Vol. 60, p. 284-293 https://doi.org/10.1016/S1872-2067(23)64628-5	journal	May 2024
A suspension-based assay and comparative detection methods for characterization of polyethylene terephthalate hydrolases Arnling Bååth, Jenny; Borch, Kim; Westh, Peter Analytical Biochemistry, Vol. 607 https://doi.org/10.1016/j.ab.2020.113873	journal	October 2020
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes Carniel, Adriano; Waldow, Vinicius de Abreu; Castro, Aline Machado de Biotechnology Advances, Vol. 52 https://doi.org/10.1016/j.biotechadv.2021.107811	journal	November 2021
The role of binding modules in enzymatic poly(ethylene terephthalate) hydrolysis at high-solids loadings Graham, Rosie; Erickson, Erika; Brizendine, Richard K. Chem Catalysis, Vol. 2, Issue 10 https://doi.org/10.1016/j.checat.2022.07.018	journal	October 2022
Rapid depolymerization of poly(ethylene terephthalate) thin films by a dual-enzyme system and its impact on material properties Tarazona, Natalia A.; Wei, Ren; Brott, Stefan Chem Catalysis, Vol. 2, Issue 12 https://doi.org/10.1016/j.checat.2022.11.004	journal	December 2022
In-Depth High-Throughput Screening of Protein Engineering Libraries by Split-GFP Direct Crude Cell Extract Data Normalization Santos-Aberturas, Javier; Dörr, Mark; Waldo, Geoffrey S. Chemistry & Biology, Vol. 22, Issue 10 https://doi.org/10.1016/j.chembiol.2015.08.014	journal	October 2015
Biosensor and chemo-enzymatic one-pot cascade applications to detect and transform PET-derived terephthalic acid in living cells Bayer, Thomas; Pfaff, Lara; Branson, Yannick iScience, Vol. 25, Issue 5 https://doi.org/10.1016/j.isci.2022.104326	journal	May 2022
Techno-economic, life-cycle, and socioeconomic impact analysis of enzymatic recycling of poly(ethylene terephthalate) Singh, Avantika; Rorrer, Nicholas A.; Nicholson, Scott R. Joule, Vol. 5, Issue 9 https://doi.org/10.1016/j.joule.2021.06.015	journal	September 2021
Turbidimetric analysis of the enzymatic hydrolysis of polyethylene terephthalate nanoparticles Wei, Ren; Oeser, Thorsten; Barth, Markus Journal of Molecular Catalysis B: Enzymatic, Vol. 103 https://doi.org/10.1016/j.molcatb.2013.08.010	journal	May 2014
Gene amplification, laboratory evolution, and biosensor screening reveal MucK as a terephthalic acid transporter in Acinetobacter baylyi ADP1 Pardo, Isabel; Jha, Ramesh K.; Bermel, Ryan E. Metabolic Engineering, Vol. 62 https://doi.org/10.1016/j.ymben.2020.09.009	journal	November 2020
Towards bio-upcycling of polyethylene terephthalate Tiso, Till; Narancic, Tanja; Wei, Ren Metabolic Engineering, Vol. 66 https://doi.org/10.1016/j.ymben.2021.03.011	journal	July 2021
Enzymes’ Power for Plastics Degradation Tournier, Vincent; Duquesne, Sophie; Guillamot, Frédérique Chemical Reviews, Vol. 123, Issue 9 https://doi.org/10.1021/acs.chemrev.2c00644	journal	March 2023
High-Throughput Analysis of Enzymatic Hydrolysis of Biodegradable Polyesters by Monitoring Cohydrolysis of a Polyester-Embedded Fluorogenic Probe Zumstein, Michael Thomas; Kohler, Hans-Peter E.; McNeill, Kristopher Environmental Science & Technology, Vol. 51, Issue 8 https://doi.org/10.1021/acs.est.6b06060	journal	February 2017
Substrate-Binding Mode of a Thermophilic PET Hydrolase and Engineering the Enzyme to Enhance the Hydrolytic Efficacy Zeng, Wei; Li, Xiuqin; Yang, Yunyun ACS Catalysis, Vol. 12, Issue 5 https://doi.org/10.1021/acscatal.1c05800	journal	February 2022
Mechanism-Based Design of Efficient PET Hydrolases Wei, Ren; von Haugwitz, Gerlis; Pfaff, Lara ACS Catalysis, Vol. 12, Issue 6 https://doi.org/10.1021/acscatal.1c05856	journal	February 2022
Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase Pfaff, Lara; Gao, Jian; Li, Zhishuai ACS Catalysis, Vol. 12, Issue 15 https://doi.org/10.1021/acscatal.2c02275	journal	July 2022
Assessment of Four Engineered PET Degrading Enzymes Considering Large-Scale Industrial Applications Arnal, Grégory; Anglade, Julien; Gavalda, Sabine ACS Catalysis, Vol. 13, Issue 20 https://doi.org/10.1021/acscatal.3c02922	journal	September 2023
Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis for Highly Efficient PET Degradation Son, Hyeoncheol Francis; Cho, In Jin; Joo, Seongjoon ACS Catalysis, Vol. 9, Issue 4 https://doi.org/10.1021/acscatal.9b00568	journal	March 2019
Current State and Perspectives Related to the Polyethylene Terephthalate Hydrolases Available for Biorecycling Kawai, Fusako; Kawabata, Takeshi; Oda, Masayuki ACS Sustainable Chemistry & Engineering, Vol. 8, Issue 24 https://doi.org/10.1021/acssuschemeng.0c01638	journal	May 2020
Particle Size Reduction of Poly(ethylene terephthalate) Increases the Rate of Enzymatic Depolymerization But Does Not Increase the Overall Conversion Extent Brizendine, Richard K.; Erickson, Erika; Haugen, Stefan J. ACS Sustainable Chemistry & Engineering, Vol. 10, Issue 28 https://doi.org/10.1021/acssuschemeng.2c01961	journal	July 2022
Ultrahigh-Throughput Directed Evolution of Polymer-Degrading Enzymes Using Yeast Display Cribari, Mario A.; Unger, Maxwell J.; Unarta, Ilona C. Journal of the American Chemical Society, Vol. 145, Issue 50, p. 27380-27389 https://doi.org/10.1021/jacs.3c08291	journal	December 2023
Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein Cabantous, Stéphanie; Terwilliger, Thomas C.; Waldo, Geoffrey S. Nature Biotechnology, Vol. 23, Issue 1 https://doi.org/10.1038/nbt1044	journal	January 2005
Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity Erickson, Erika; Gado, Japheth E.; Avilán, Luisana Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-35237-x	journal	December 2022
An engineered PET depolymerase to break down and recycle plastic bottles Tournier, V.; Topham, C. M.; Gilles, A. Nature, Vol. 580, Issue 7802 https://doi.org/10.1038/s41586-020-2149-4	journal	April 2020
Machine learning-aided engineering of hydrolases for PET depolymerization Lu, Hongyuan; Diaz, Daniel J.; Czarnecki, Natalie J. Nature, Vol. 604, Issue 7907 https://doi.org/10.1038/s41586-022-04599-z	journal	April 2022
Macromolecular modeling and design in Rosetta: recent methods and frameworks Leman, Julia Koehler; Weitzner, Brian D.; Lewis, Steven M. Nature Methods, Vol. 17, Issue 7 https://doi.org/10.1038/s41592-020-0848-2	journal	June 2020
An absorbance method for analysis of enzymatic degradation kinetics of poly(ethylene terephthalate) films Zhong-Johnson, En Ze Linda; Voigt, Christopher A.; Sinskey, Anthony J. Scientific Reports, Vol. 11, Issue 1 https://doi.org/10.1038/s41598-020-79031-5	journal	January 2021
Engineering an efficient and bright split Corynactis californica green fluorescent protein Nguyen, Hau B.; Terwilliger, Thomas C.; Waldo, Geoffrey S. Scientific Reports, Vol. 11, Issue 1 https://doi.org/10.1038/s41598-021-98149-8	journal	September 2021
Chemical and biological catalysis for plastics recycling and upcycling Ellis, Lucas D.; Rorrer, Nicholas A.; Sullivan, Kevin P. Nature Catalysis, Vol. 4, Issue 7 https://doi.org/10.1038/s41929-021-00648-4	journal	July 2021
Directed evolution of an efficient and thermostable PET depolymerase Bell, Elizabeth L.; Smithson, Ross; Kilbride, Siobhan Nature Catalysis, Vol. 5, Issue 8 https://doi.org/10.1038/s41929-022-00821-3	journal	August 2022
Life cycle assessment of enzymatic poly(ethylene terephthalate) recycling Uekert, Taylor; DesVeaux, Jason S.; Singh, Avantika Green Chemistry, Vol. 24, Issue 17 https://doi.org/10.1039/D2GC02162E	journal	January 2022
A flexible kinetic assay efficiently sorts prospective biocatalysts for PET plastic subunit hydrolysis Lusty Beech, Jessica; Clare, Rita; Kincannon, William M. RSC Advances, Vol. 12, Issue 13 https://doi.org/10.1039/D2RA00612J	journal	January 2022
Characterization of polymer properties and identification of additives in commercially available research plastics Cuthbertson, Amy A.; Lincoln, Clarissa; Miscall, Joel Green Chemistry, Vol. 26, Issue 12, p. 7067-7090 https://doi.org/10.1039/D4GC00659C	journal	January 2024
Characterization and engineering of a two-enzyme system for plastics depolymerization Knott, Brandon C.; Erickson, Erika; Allen, Mark D. Proceedings of the National Academy of Sciences, Vol. 117, Issue 41 https://doi.org/10.1073/pnas.2006753117	journal	September 2020
Identification and Characterization of Bacterial Cutinase Chen, Sheng; Tong, Xing; Woodard, Ronald W. Journal of Biological Chemistry, Vol. 283, Issue 38 https://doi.org/10.1074/jbc.M800848200	journal	July 2008
UniRef clusters: a comprehensive and scalable alternative for improving sequence similarity searches Suzek, B. E.; Wang, Y.; Huang, H. Bioinformatics, Vol. 31, Issue 6 https://doi.org/10.1093/bioinformatics/btu739	journal	November 2014
HMMER web server: 2015 update Finn, Robert D.; Clements, Jody; Arndt, William Nucleic Acids Research, Vol. 43, Issue W1 https://doi.org/10.1093/nar/gkv397	journal	May 2015
Split green fluorescent protein as a modular binding partner for protein crystallization Nguyen, Hau B.; Hung, Li-Wei; Yeates, Todd O. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 12 https://doi.org/10.1107/s0907444913024608	journal	November 2013
Agar plate‐based screening methods for the identification of polyester hydrolysis by Pseudomonas species Molitor, Rebecka; Bollinger, Alexander; Kubicki, Sonja Microbial Biotechnology, Vol. 13, Issue 1 https://doi.org/10.1111/1751-7915.13418	journal	January 2020
Production, use, and fate of all plastics ever made Geyer, Roland; Jambeck, Jenna R.; Law, Kara Lavender Science Advances, Vol. 3, Issue 7 https://doi.org/10.1126/sciadv.1700782	journal	July 2017
A bacterium that degrades and assimilates poly(ethylene terephthalate) Yoshida, Shosuke; Hiraga, Kazumi; Takehana, Toshihiko Science, Vol. 351, Issue 6278 https://doi.org/10.1126/science.aad6359	journal	March 2016
Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach Sulaiman, Sintawee; Yamato, Saya; Kanaya, Eiko Applied and Environmental Microbiology, Vol. 78, Issue 5 https://doi.org/10.1128/AEM.06725-11	journal	December 2011
Improving Protein Folding Efficiency by Directed Evolution Using the GFP Folding Reporter Waldo, Geoffrey S. Directed Enzyme Evolution https://doi.org/10.1385/1-59259-396-8:343	book	January 2003
Global Plastics Outlook: Policy Scenarios to 2060 https://doi.org/10.1787/aa1edf33-en	book	June 2022
Microbial Polyethylene Terephthalate Hydrolases: Current and Future Perspectives Carr, Clodagh M.; Clarke, David J.; Dobson, Alan D. W. Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.571265	journal	November 2020

Similar Records

Protocol for engineering poly(ethylene terephthalate) hydrolases via directed evolution using a high-throughput screening assay

Journal Article · 2025 · Star Protocols · OSTI ID:2572546

Groseclose, Thomas Michael; Taylor, Zoe Kyoko; Lujan, Lexy Amber Esmith; +2 more

Development of a High Throughput Platform for Screening Glycoside Hydrolases Based on Oxime-NIMS

Journal Article · 2015 · Frontiers in Bioengineering and Biotechnology · OSTI ID:1512119

Deng, Kai; Guenther, Joel M.; Gao, Jian; +17 more

Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity

Journal Article · 2022 · Nature Communications · OSTI ID:1907620

Erickson, Erika; Gado, Japheth E.; Avilán, Luisana; +18 more

Title: A High-Throughput Screening Platform for Engineering Poly(ethylene Terephthalate) Hydrolases

Citation Formats

References (54)

Similar Records

Related Subjects