Arabidopsis trigalactosyldiacylglycerol1 mutants reveal a critical role for phosphtidylcholine remodeling in lipid homeostasis

Fan, Jilian; Sah, Saroj Kumar; Lemes Jorge, Gabriel; Blanford, Jantana; Xie, Dongling; Yu, Linhui; Thelen, Jay; Shanklin, John; Xu, Changcheng

doi:10.1111/tpj.17020

Title: Arabidopsis trigalactosyldiacylglycerol1 mutants reveal a critical role for phosphtidylcholine remodeling in lipid homeostasis

Journal Article · 13 September 2024 · The Plant Journal

DOI: https://doi.org/10.1111/tpj.17020 · OSTI ID:2440523

Fan, Jilian ^[1]; Sah, Saroj Kumar ^[1]; Lemes Jorge, Gabriel ^[2]; Blanford, Jantana ^[1]; Xie, Dongling ^[1]; Yu, Linhui ^[1];

^[2];

^[1];

^[1]

Brookhaven National Laboratory (BNL), Upton, NY (United States)
Univ. of Missouri, Columbia, MO (United States). Christopher S. Bond Life Sciences Center

Lipid remodeling plays a critical role in plant response to abiotic stress and metabolic perturbations. Key steps in this process involve modifications of phosphatidylcholine (PC) acyl chains mediated by lysophosphatidylcholine: acyl-CoA acyltransferases (LPCATs) and phosphatidylcholine: diacylglycerol cholinephosphotransferase (ROD1). To assess their importance in lipid homeostasis, we took advantage of the trigalactosyldiacylglycerol1 (tgd1) mutant that exhibits marked increases in fatty acid synthesis and fatty acid flux through PC due to a block in inter-organelle lipid trafficking. Here, we showed that the increased fatty acid synthesis in tgd1 is due to posttranslational activation of the plastidic acetyl-coenzyme A carboxylase. Genetic analysis showed that knockout of LPCAT1 and 2 resulted in a lethal phenotype in tgd1. In addition, plants homozygous for lpcat2 and heterozygous for lpcat1 in the tgd1 background showed reduced levels of PC and triacylglycerols (TAG) and alterations in their fatty acid profiles. We further showed that disruption of ROD1 in tgd1 resulted in changes in fatty acid composition of PC and TAG, decreased leaf TAG content and reduced seedling growth. Combined, our results reveal a critical role of LPCATs and ROD1 in maintaining cellular lipid homeostasis under conditions, in which fatty acid production largely exceeds the cellular demand for membrane lipid synthesis.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: SC0012704

OSTI ID:: 2440523

Report Number(s):: BNL--226029-2024-JAAM

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Journal Issue: 2 Vol. 120; ISSN 0960-7412

Publisher:: Society for Experimental BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Arabidopsis thaliana
fatty acid synthesis
lipid remodeling
lipid trafﬁcking
phosphatidylcholine
trigalactosyldiacyl-glycerol1

Title: Arabidopsis trigalactosyldiacylglycerol1 mutants reveal a critical role for phosphtidylcholine remodeling in lipid homeostasis

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