Role of ammonia-lyases in the synthesis of the dithiomethylamine ligand during [FeFe]-hydrogenase maturation

Pagnier, Adrien; Balci, Batuhan; Shepard, Eric M.; Yang, Hao; Drena, Alex; Holliday, Gemma L.; Hoffman, Brian M.; Broderick, William E.; Broderick, Joan B.

doi:10.1016/j.jbc.2024.107760

Title: Role of ammonia-lyases in the synthesis of the dithiomethylamine ligand during [FeFe]-hydrogenase maturation

Journal Article · 01 October 2024 · Journal of Biological Chemistry

DOI: https://doi.org/10.1016/j.jbc.2024.107760 · OSTI ID:2440440

;

; Yang, Hao; Drena, Alex; Holliday, Gemma L.; Hoffman, Brian M.; Broderick, William E.;

Not Available

View Journal Article

Cite

Export

Save

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0005404

OSTI ID:: 2440440

Journal Information:: Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Journal Issue: 10 Vol. 300; ISSN 0021-9258

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (75)

[FeFe]-Hydrogenase Cyanide Ligands Derived From S-Adenosylmethionine-Dependent Cleavage of Tyrosine Driesener, Rebecca C.; Challand, Martin R.; McGlynn, Shawn E. Angewandte Chemie International Edition, Vol. 49, Issue 9 https://doi.org/10.1002/anie.200907047	journal	January 2010
Cell-free synthesis and maturation of [FeFe] hydrogenases Boyer, Marcus E.; Stapleton, James A.; Kuchenreuther, Jon M. Biotechnology and Bioengineering, Vol. 99, Issue 1 https://doi.org/10.1002/bit.21511	journal	January 2008
The [4Fe‐4S]‐Cluster of HydF is not Required for the Binding and Transfer of the Diiron Site of [FeFe]‐Hydrogenases Haas, Rieke; Engelbrecht, Vera; Lampret, Oliver ChemBioChem, Vol. 24, Issue 11 https://doi.org/10.1002/cbic.202300222	journal	May 2023
Q-Band Pulsed Electron Spin-Echo Spectrometer and Its Application to ENDOR and ESEEM Davoust, Clark E.; Doan, Peter E.; Hoffman, Brian M. Journal of Magnetic Resonance, Series A, Vol. 119, Issue 1 https://doi.org/10.1006/jmra.1996.0049	journal	March 1996
The mitochondrial glycine cleavage system Kikuchi, G.; Hiraga, K. Molecular and Cellular Biochemistry, Vol. 45, Issue 3 https://doi.org/10.1007/BF00230082	journal	June 1982
The glycine cleavage system: Composition, reaction mechanism, and physiological significance Kikuchi, Goro Molecular and Cellular Biochemistry, Vol. 1, Issue 2 https://doi.org/10.1007/BF01659328	journal	September 1973
An EPR/HYSCORE, Mössbauer, and resonance Raman study of the hydrogenase maturation enzyme HydF: a model for N-coordination to [4Fe–4S] clusters Berggren, Gustav; Garcia-Serres, Ricardo; Brazzolotto, Xavier JBIC Journal of Biological Inorganic Chemistry, Vol. 19, Issue 1 https://doi.org/10.1007/s00775-013-1062-9	journal	November 2013
H-cluster assembly intermediates built on HydF by the radical SAM enzymes HydE and HydG Byer, Amanda S.; Shepard, Eric M.; Ratzloff, Michael W. JBIC Journal of Biological Inorganic Chemistry, Vol. 24, Issue 6 https://doi.org/10.1007/s00775-019-01709-7	journal	September 2019
[FeFe]-hydrogenase maturation: H-cluster assembly intermediates tracked by electron paramagnetic resonance, infrared, and X-ray absorption spectroscopy Németh, Brigitta; Senger, Moritz; Redman, Holly J. JBIC Journal of Biological Inorganic Chemistry, Vol. 25, Issue 5 https://doi.org/10.1007/s00775-020-01799-8	journal	July 2020
The purification and properties of aspartase from Escherichia coli Rudolph, Frederick B.; Fromm, Herbert J. Archives of Biochemistry and Biophysics, Vol. 147, Issue 1 https://doi.org/10.1016/0003-9861(71)90313-4	journal	November 1971
The Stoichiometry and Kinetics of the Inducible Cysteine Desulfhydrase from Salmonella typhimurium Kredich, Nicholas M.; Foote, Linda J.; Keenan, Bruce S. Journal of Biological Chemistry, Vol. 248, Issue 17 https://doi.org/10.1016/S0021-9258(19)43526-6	journal	September 1973
Glycine synthase of the purinolytic bacterium, Clostridium acidiurici. Purification of the glycine-CO2 exchange system. Gariboldi, R. T.; Drake, H. L. Journal of Biological Chemistry, Vol. 259, Issue 10 https://doi.org/10.1016/S0021-9258(20)82108-5	journal	May 1984
Desulfovibrio desulfuricans iron hydrogenase: the structure shows unusual coordination to an active site Fe binuclear center Nicolet, Yvain; Piras, Claudine; Legrand, Pierre Structure, Vol. 7, Issue 1, p. 13-23 https://doi.org/10.1016/S0969-2126(99)80005-7	journal	January 1999
The primary and three-dimensional structures of a nine-haem cytochrome c from Desulfovibrio desulfuricans ATCC 27774 reveal a new member of the Hmc family Matias, Pedro M.; Coelho, Ricardo; Pereira, Inês AC Structure, Vol. 7, Issue 2 https://doi.org/10.1016/S0969-2126(99)80019-7	journal	February 1999
IscS from Archaeoglobus fulgidus has no desulfurase activity but may provide a cysteine ligand for [Fe 2 S 2 ] cluster assembly Pagnier, Adrien; Nicolet, Yvain; Fontecilla-Camps, Juan C. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1853, Issue 6 https://doi.org/10.1016/j.bbamcr.2014.10.015	journal	June 2015
Quantitative analysis of glycine related metabolic pathways for one-carbon synthetic biology Hong, Yaeseong; Ren, Jie; Zhang, Xinyi Current Opinion in Biotechnology, Vol. 64 https://doi.org/10.1016/j.copbio.2019.10.001	journal	August 2020
HydF as a scaffold protein in [FeFe] hydrogenase H-cluster biosynthesis McGlynn, Shawn E.; Shepard, Eric M.; Winslow, Mark A. FEBS Letters, Vol. 582, Issue 15 https://doi.org/10.1016/j.febslet.2008.04.063	journal	May 2008
Iron-sulfur cluster coordination in the [FeFe]-hydrogenase H cluster biosynthetic factor HydF Joshi, Neelambari; Shepard, Eric M.; Byer, Amanda S. FEBS Letters, Vol. 586, Issue 22 https://doi.org/10.1016/j.febslet.2012.09.028	journal	October 2012
EFI-EST, EFI-GNT, and EFI-CGFP: Enzyme Function Initiative (EFI) Web Resource for Genomic Enzymology Tools Oberg, Nils; Zallot, Rémi; Gerlt, John A. Journal of Molecular Biology, Vol. 435, Issue 14 https://doi.org/10.1016/j.jmb.2023.168018	journal	July 2023
Insights into [FeFe]-Hydrogenase Structure, Mechanism, and Maturation Mulder, David W.; Shepard, Eric M.; Meuser, Jonathan E. Structure, Vol. 19, Issue 8 https://doi.org/10.1016/j.str.2011.06.008	journal	August 2011
A Redox Active [2Fe-2S] Cluster on the Hydrogenase Maturase HydF Shepard, Eric M.; Byer, Amanda S.; Betz, Jeremiah N. Biochemistry, Vol. 55, Issue 25 https://doi.org/10.1021/acs.biochem.6b00528	journal	June 2016
Electron Spin Relaxation and Biochemical Characterization of the Hydrogenase Maturase HydF: Insights into [2Fe-2S] and [4Fe-4S] Cluster Communication and Hydrogenase Activation Shepard, Eric M.; Byer, Amanda S.; Aggarwal, Priyanka Biochemistry, Vol. 56, Issue 25 https://doi.org/10.1021/acs.biochem.7b00169	journal	May 2017
Iron–Sulfur Cluster States of the Hydrogenase Maturase HydF Shepard, Eric M.; Byer, Amanda S.; Broderick, Joan B. Biochemistry, Vol. 56, Issue 36 https://doi.org/10.1021/acs.biochem.7b00735	journal	August 2017
The EFI Web Resource for Genomic Enzymology Tools: Leveraging Protein, Genome, and Metagenome Databases to Discover Novel Enzymes and Metabolic Pathways Zallot, Rémi; Oberg, Nils; Gerlt, John A. Biochemistry, Vol. 58, Issue 41 https://doi.org/10.1021/acs.biochem.9b00735	journal	September 2019
Current State of [FeFe]-Hydrogenase Research: Biodiversity and Spectroscopic Investigations Land, Henrik; Senger, Moritz; Berggren, Gustav ACS Catalysis, Vol. 10, Issue 13 https://doi.org/10.1021/acscatal.0c01614	journal	May 2020
L-Aspartase from Escherichia coli: substrate specificity and role of divalent metal ions Falzone, Christopher J.; Karsten, William E.; Conley, Judith D. Biochemistry, Vol. 27, Issue 26 https://doi.org/10.1021/bi00426a004	journal	December 1988
[FeFe]-Hydrogenase Maturation: Insights into the Role HydE Plays in Dithiomethylamine Biosynthesis Betz, Jeremiah N.; Boswell, Nicholas W.; Fugate, Corey J. Biochemistry, Vol. 54, Issue 9 https://doi.org/10.1021/bi501205e	journal	March 2015
Activation of HydA ^ΔEFG Requires a Preformed [4Fe-4S] Cluster Mulder, David W.; Ortillo, Danilo O.; Gardenghi, David J. Biochemistry, Vol. 48, Issue 26 https://doi.org/10.1021/bi9000563	journal	July 2009
Hydrogenases Lubitz, Wolfgang; Ogata, Hideaki; Rüdiger, Olaf Chemical Reviews, Vol. 114, Issue 8 https://doi.org/10.1021/cr4005814	journal	March 2014
Ligand spin densities in blue copper proteins by q-band proton and nitrogen-14 ENDOR spectroscopy Werst, Melanie M.; Davoust, Clark E.; Hoffman, Brian M. Journal of the American Chemical Society, Vol. 113, Issue 5 https://doi.org/10.1021/ja00005a011	journal	February 1991
Electron-Nuclear Double Resonance Spectroscopic Evidence That S -Adenosylmethionine Binds in Contact with the Catalytically Active [4Fe−4S] ⁺ Cluster of Pyruvate Formate-Lyase Activating Enzyme Walsby, Charles J.; Hong, Wei; Broderick, William E. Journal of the American Chemical Society, Vol. 124, Issue 12 https://doi.org/10.1021/ja012034s	journal	March 2002
An Anchoring Role for FeS Clusters: Chelation of the Amino Acid Moiety of S-Adenosylmethionine to the Unique Iron Site of the [4Fe−4S] Cluster of Pyruvate Formate-Lyase Activating Enzyme Walsby, Charles J.; Ortillo, Danilo; Broderick, William E. Journal of the American Chemical Society, Vol. 124, Issue 38 https://doi.org/10.1021/ja027078v	journal	September 2002
[FeFe]-Hydrogenase Maturation: HydG-Catalyzed Synthesis of Carbon Monoxide Shepard, Eric M.; Duffus, Benjamin R.; George, Simon J. Journal of the American Chemical Society, Vol. 132, Issue 27 https://doi.org/10.1021/ja1012273	journal	July 2010
Investigations on the Role of Proton-Coupled Electron Transfer in Hydrogen Activation by [FeFe]-Hydrogenase Mulder, David W.; Ratzloff, Michael W.; Bruschi, Maurizio Journal of the American Chemical Society, Vol. 136, Issue 43 https://doi.org/10.1021/ja508629m	journal	October 2014
Radical SAM Enzyme HydE Generates Adenosylated Fe(I) Intermediates En Route to the [FeFe]-Hydrogenase Catalytic H-Cluster Tao, Lizhi; Pattenaude, Scott A.; Joshi, Sumedh Journal of the American Chemical Society, Vol. 142, Issue 24 https://doi.org/10.1021/jacs.0c03802	journal	May 2020
Crystal Structure of the [FeFe]-Hydrogenase Maturase HydE Bound to Complex-B Rohac, Roman; Martin, Lydie; Liu, Liang Journal of the American Chemical Society, Vol. 143, Issue 22 https://doi.org/10.1021/jacs.1c03367	journal	May 2021
Organometallic Fe₂(μ-SH)₂(CO)₄(CN)₂ Cluster Allows the Biosynthesis of the [FeFe]-Hydrogenase with Only the HydF Maturase Zhang, Yu; Tao, Lizhi; Woods, Toby J. Journal of the American Chemical Society, Vol. 144, Issue 4 https://doi.org/10.1021/jacs.1c12506	journal	January 2022
Direct Observation of an Iron-Bound Terminal Hydride in [FeFe]-Hydrogenase by Nuclear Resonance Vibrational Spectroscopy Reijerse, Edward J.; Pham, Cindy C.; Pelmenschikov, Vladimir Journal of the American Chemical Society, Vol. 139, Issue 12 https://doi.org/10.1021/jacs.7b00686	journal	March 2017
Alkaline Degradation of Cystine, Glutathione and Sulphur-containing Proteins Robbins, F. M.; Fioriti, Joseph A. Nature, Vol. 200, Issue 4906 https://doi.org/10.1038/200577a0	journal	November 1963
Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydAΔEFG Mulder, David W.; Boyd, Eric S.; Sarma, Ranjana Nature, Vol. 465, Issue 7295 https://doi.org/10.1038/nature08993	journal	April 2010
Carbon–sulfur bond-forming reaction catalysed by the radical SAM enzyme HydE Rohac, Roman; Amara, Patricia; Benjdia, Alhosna Nature Chemistry, Vol. 8, Issue 5 https://doi.org/10.1038/nchem.2490	journal	April 2016
Structural and functional characterization of the hydrogenase-maturation HydF protein Caserta, Giorgio; Pecqueur, Ludovic; Adamska-Venkatesh, Agnieszka Nature Chemical Biology, Vol. 13, Issue 7 https://doi.org/10.1038/nchembio.2385	journal	May 2017
The ecology and biotechnology of sulphate-reducing bacteria Muyzer, Gerard; Stams, Alfons J. M. Nature Reviews Microbiology, Vol. 6, Issue 6 https://doi.org/10.1038/nrmicro1892	journal	May 2008
A [4Fe–4S]-Fe(CO)(CN)-l-cysteine intermediate is the first organometallic precursor in [FeFe] hydrogenase H-cluster bioassembly Rao, Guodong; Tao, Lizhi; Suess, Daniel L. M. Nature Chemistry, Vol. 10, Issue 5 https://doi.org/10.1038/s41557-018-0026-7	journal	April 2018
Identifying conformational changes with site-directed spin labeling reveals that the GTPase domain of HydF is a molecular switch Galazzo, Laura; Maso, Lorenzo; De Rosa, Edith Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-01886-y	journal	May 2017
From protein engineering to artificial enzymes – biological and biomimetic approaches towards sustainable hydrogen production Esmieu, C.; Raleiras, P.; Berggren, G. Sustainable Energy & Fuels, Vol. 2, Issue 4 https://doi.org/10.1039/C7SE00582B	journal	January 2018
The value of enzymes in solar fuels research – efficient electrocatalysts through evolution Evans, Rhiannon M.; Siritanaratkul, Bhavin; Megarity, Clare F. Chemical Society Reviews, Vol. 48, Issue 7 https://doi.org/10.1039/C8CS00546J	journal	January 2019
Compositional and structural insights into the nature of the H-cluster precursor on HydF Scott, Anna G.; Szilagyi, Robert K.; Mulder, David W. Dalton Transactions, Vol. 47, Issue 28 https://doi.org/10.1039/C8DT01654B	journal	January 2018
Iron–sulphur cluster biogenesis via the SUF pathway Bai, Y.; Chen, T.; Happe, T. Metallomics, Vol. 10, Issue 8 https://doi.org/10.1039/C8MT00150B	journal	January 2018
Serine is the molecular source of the NH(CH ₂ ) ₂ bridgehead moiety of the in vitro assembled [FeFe] hydrogenase H-cluster Rao, Guodong; Tao, Lizhi; Britt, R. David Chemical Science, Vol. 11, Issue 5 https://doi.org/10.1039/C9SC05900H	journal	January 2020
[FeFe]-Hydrogenases: maturation and reactivity of enzymatic systems and overview of biomimetic models Kleinhaus, Julian T.; Wittkamp, Florian; Yadav, Shanika Chemical Society Reviews, Vol. 50, Issue 3 https://doi.org/10.1039/D0CS01089H	journal	January 2021
HydG, the “dangler” iron, and catalytic production of free CO and CN ⁻ : implications for [FeFe]-hydrogenase maturation Shepard, Eric M.; Impano, Stella; Duffus, Benjamin R. Dalton Transactions, Vol. 50, Issue 30 https://doi.org/10.1039/D1DT01359A	journal	January 2021
Global profiling of Shewanella oneidensis MR-1: Expression of hypothetical genes and improved functional annotations Kolker, E.; Picone, A. F.; Galperin, M. Y. Proceedings of the National Academy of Sciences, Vol. 102, Issue 6 https://doi.org/10.1073/pnas.0409111102	journal	January 2005
Synthesis of the 2Fe subcluster of the [FeFe]-hydrogenase H cluster on the HydF scaffold Shepard, E. M.; McGlynn, S. E.; Bueling, A. L. Proceedings of the National Academy of Sciences, Vol. 107, Issue 23 https://doi.org/10.1073/pnas.1001937107	journal	May 2010
Cysteine as a ligand platform in the biosynthesis of the FeFe hydrogenase H cluster Suess, Daniel L. M.; Bürstel, Ingmar; De La Paz, Liliana Proceedings of the National Academy of Sciences, Vol. 112, Issue 37 https://doi.org/10.1073/pnas.1508440112	journal	August 2015
CO and CN ⁻ syntheses by [FeFe]-hydrogenase maturase HydG are catalytically differentiated events Pagnier, Adrien; Martin, Lydie; Zeppieri, Laura Proceedings of the National Academy of Sciences, Vol. 113, Issue 1 https://doi.org/10.1073/pnas.1515842113	journal	December 2015
Crystal Structure of Aminomethyltransferase in Complex with Dihydrolipoyl-H-Protein of the Glycine Cleavage System: IMPLICATIONS FOR RECOGNITION OF LIPOYL PROTEIN SUBSTRATE, DISEASE-RELATED MUTATIONS, AND REACTION MECHANISM Okamura-Ikeda, Kazuko; Hosaka, Harumi; Maita, Nobuo Journal of Biological Chemistry, Vol. 285, Issue 24 https://doi.org/10.1074/jbc.M110.110718	journal	April 2010
Biochemical Analysis of the Interactions between the Proteins Involved in the [FeFe]-Hydrogenase Maturation Process Vallese, Francesca; Berto, Paola; Ruzzene, Maria Journal of Biological Chemistry, Vol. 287, Issue 43 https://doi.org/10.1074/jbc.M112.388900	journal	August 2012
Discovery of Two Novel Radical S -Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase Posewitz, Matthew C.; King, Paul W.; Smolinski, Sharon L. Journal of Biological Chemistry, Vol. 279, Issue 24 https://doi.org/10.1074/jbc.M403206200	journal	April 2004
Escherichia coli L-Serine Deaminase Requires a [4Fe-4S] Cluster in Catalysis Cicchillo, Robert M.; Baker, Melissa A.; Schnitzer, Eric J. Journal of Biological Chemistry, Vol. 279, Issue 31 https://doi.org/10.1074/jbc.M404381200	journal	July 2004
The [Fe-Fe]-Hydrogenase Maturation Protein HydF from Thermotoga maritima Is a GTPase with an Iron-Sulfur Cluster Brazzolotto, Xavier; Rubach, Jon K.; Gaillard, Jacques Journal of Biological Chemistry, Vol. 281, Issue 2 https://doi.org/10.1074/jbc.M510310200	journal	November 2005
The maturase HydF enables [FeFe] hydrogenase assembly via transient, cofactor-dependent interactions Németh, Brigitta; Land, Henrik; Magnuson, Ann Journal of Biological Chemistry, Vol. 295, Issue 33 https://doi.org/10.1074/jbc.RA119.011419	journal	August 2020
UniProt: the Universal Protein Knowledgebase in 2023 Nucleic Acids Research, Vol. 51, Issue D1 https://doi.org/10.1093/nar/gkac1052	journal	November 2022
Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks Shannon, P. Genome Research, Vol. 13, Issue 11 https://doi.org/10.1101/gr.1239303	journal	November 2003
Microbial consumption of zero-valence sulfur in marine benthic habitats: Microbial S Pjevac, Petra; Kamyshny, Alexey; Dyksma, Stefan Environmental Microbiology, Vol. 16, Issue 11 https://doi.org/10.1111/1462-2920.12410	journal	February 2014
A Radical Intermediate in Tyrosine Scission to the CO and CN- Ligands of FeFe Hydrogenase Kuchenreuther, J. M.; Myers, W. K.; Stich, T. A. Science, Vol. 342, Issue 6157 https://doi.org/10.1126/science.1241859	journal	October 2013
The HydG Enzyme Generates an Fe(CO)2(CN) Synthon in Assembly of the FeFe Hydrogenase H-Cluster Kuchenreuther, J. M.; Myers, W. K.; Suess, D. L. M. Science, Vol. 343, Issue 6169 https://doi.org/10.1126/science.1246572	journal	January 2014
Bacterial Manganese Reduction and Growth with Manganese Oxide as the Sole Electron Acceptor Myers, C. R.; Nealson, K. H. Science, Vol. 240, Issue 4857 https://doi.org/10.1126/science.240.4857.1319	journal	June 1988
X-ray Crystal Structure of the Fe-Only Hydrogenase (CpI) from Clostridium pasteurianum to 1.8 Angstrom Resolution Peters, J. W. Science, Vol. 282, Issue 5395 https://doi.org/10.1126/science.282.5395.1853	journal	December 1998
Hydrogen Metabolism in Shewanella oneidensis MR-1 Meshulam-Simon, G.; Behrens, S.; Choo, A. D. Applied and Environmental Microbiology, Vol. 73, Issue 4 https://doi.org/10.1128/AEM.01588-06	journal	December 2006
Deficiency in l-Serine Deaminase Interferes with One-Carbon Metabolism and Cell Wall Synthesis in Escherichia coli K-12 Zhang, Xiao; El-Hajj, Ziad W.; Newman, Elaine Journal of Bacteriology, Vol. 192, Issue 20 https://doi.org/10.1128/JB.00748-10	journal	August 2010
Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System King, P. W.; Posewitz, M. C.; Ghirardi, M. L. Journal of Bacteriology, Vol. 188, Issue 6 https://doi.org/10.1128/JB.188.6.2163-2172.2006	journal	March 2006
Fe(III) and S0 reduction by Pelobacter carbinolicus. Lovley, D. R.; Phillips, E. J.; Lonergan, D. J. Applied and environmental microbiology, Vol. 61, Issue 6 https://doi.org/10.1128/aem.61.6.2132-2138.1995	journal	January 1995
Glycine cleavage system: reaction mechanism, physiological significance, and hyperglycinemia Kikuchi, Goro; Motokawa, Yutaro; Yoshida, Tadashi Proceedings of the Japan Academy, Series B, Vol. 84, Issue 7 https://doi.org/10.2183/pjab.84.246	journal	January 2008
Pathways of carbon oxidation in an Arctic fjord sediment (Svalbard) and isolation of psychrophilic and psychrotolerant Fe(III)-reducing bacteria Vandieken, V.; Finke, N.; Jørgensen, Bb Marine Ecology Progress Series, Vol. 322 https://doi.org/10.3354/meps322029	journal	September 2006

Similar Records

[FeFe]-Hydrogenase In Vitro Maturation

Journal Article · 2022 · Angewandte Chemie (International Edition) · OSTI ID:2419698

Pagnier, Adrien; Balci, Batuhan; Shepard, Eric M.; +2 more

[FeFe]-Hydrogenase: Defined Lysate-Free Maturation Reveals a Key Role for Lipoyl-H-Protein in DTMA Ligand Biosynthesis

Journal Article · 2022 · Angewandte Chemie (International Edition) · OSTI ID:1976282

Pagnier, Adrien; Balci, Batuhan; Shepard, Eric M.; +7 more

Insights into [FeFe]-Hydrogenase Structure, Mechanism, and Maturation

Journal Article · 2011 · Structure · OSTI ID:1198409

Mulder, David W.; Shepard, Eric M.; Meuser, Jonathan E.; +5 more

Title: Role of ammonia-lyases in the synthesis of the dithiomethylamine ligand during [FeFe]-hydrogenase maturation

Citation Formats

References (75)

Similar Records

Related Subjects