Strangers in a foreign land: ‘Yeastizing’ plant enzymes

Van Gelder, Kristen; Lindner, Steffen N.; Hanson, Andrew D.; Zhou, Juannan

doi:10.1111/1751-7915.14525

Title: Strangers in a foreign land: ‘Yeastizing’ plant enzymes

Journal Article · 01 September 2024 · Microbial Biotechnology (Online)

DOI: https://doi.org/10.1111/1751-7915.14525 · OSTI ID:2439084

Van Gelder, Kristen ^[1]; Lindner, Steffen N. ^[2]; Hanson, Andrew D. ^[1];

^[3]

Horticultural Sciences Department University of Florida Gainesville Florida USA
Department of Systems and Synthetic Metabolism Max Planck Institute of Molecular Plant Physiology Potsdam Germany, Department of Biochemistry Charité Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt‐Universität Berlin Germany
Department of Biology University of Florida Gainesville Florida USA

Abstract Expressing plant metabolic pathways in microbial platforms is an efficient, cost‐effective solution for producing many desired plant compounds. As eukaryotic organisms, yeasts are often the preferred platform. However, expression of plant enzymes in a yeast frequently leads to failure because the enzymes are poorly adapted to the foreign yeast cellular environment. Here, we first summarize the current engineering approaches for optimizing performance of plant enzymes in yeast. A critical limitation of these approaches is that they are labour‐intensive and must be customized for each individual enzyme, which significantly hinders the establishment of plant pathways in cellular factories. In response to this challenge, we propose the development of a cost‐effective computational pipeline to redesign plant enzymes for better adaptation to the yeast cellular milieu. This proposition is underpinned by compelling evidence that plant and yeast enzymes exhibit distinct sequence features that are generalizable across enzyme families. Consequently, we introduce a data‐driven machine learning framework designed to extract ‘yeastizing’ rules from natural protein sequence variations, which can be broadly applied to all enzymes. Additionally, we discuss the potential to integrate the machine learning model into a full design‐build‐test cycle.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0020153

OSTI ID:: 2439084

Journal Information:: Microbial Biotechnology (Online), Journal Name: Microbial Biotechnology (Online) Journal Issue: 9 Vol. 17; ISSN 1751-7915

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

References (88)

Codon harmonization – going beyond the speed limit for protein expression Mignon, Charlotte; Mariano, Natacha; Stadthagen, Gustavo FEBS Letters, Vol. 592, Issue 9 https://doi.org/10.1002/1873-3468.13046	journal	April 2018
Directed evolution: Past, present, and future Cobb, Ryan E.; Chao, Ran; Zhao, Huimin AIChE Journal, Vol. 59, Issue 5 https://doi.org/10.1002/aic.13995	journal	January 2013
Determination of the cytosolic free NAD/NADH ratio in Saccharomyces cerevisiae under steady-state and highly dynamic conditions Canelas, André B.; van Gulik, Walter M.; Heijnen, Joseph J. Biotechnology and Bioengineering, Vol. 100, Issue 4 https://doi.org/10.1002/bit.21813	journal	July 2008
Predotar: A tool for rapidly screening proteomes forN-terminal targeting sequences Small, Ian; Peeters, Nemo; Legeai, Fabrice PROTEOMICS, Vol. 4, Issue 6 https://doi.org/10.1002/pmic.200300776	journal	June 2004
Predicting Subcellular Localization of Proteins Based on their N-terminal Amino Acid Sequence Emanuelsson, Olof; Nielsen, Henrik; Brunak, Søren Journal of Molecular Biology, Vol. 300, Issue 4 https://doi.org/10.1006/jmbi.2000.3903	journal	July 2000
Recombinant Protein Production in Yeasts Mattanovich, Diethard; Branduardi, Paola; Dato, Laura Recombinant Gene Expression https://doi.org/10.1007/978-1-61779-433-9_17	book	November 2011
A human gene, hSGT1, can substitute for GCR2, which encodes a general regulatory factor of glycolytic gene expression in Saccharomyces cerevisiae Sato, T.; Jigami, Y.; Suzuki, T. Molecular and General Genetics MGG, Vol. 260, Issue 6 https://doi.org/10.1007/s004380050926	journal	January 1999
Codon usage bias Parvathy, Sujatha Thankeswaran; Udayasuriyan, Varatharajalu; Bhadana, Vijaipal Molecular Biology Reports, Vol. 49, Issue 1 https://doi.org/10.1007/s11033-021-06749-4	journal	November 2021
Codon optimization of the calf prochymosin gene and its expression in Kluyveromyces lactis Feng, Zhen; Zhang, Lanwei; Han, Xue World Journal of Microbiology and Biotechnology, Vol. 26, Issue 5 https://doi.org/10.1007/s11274-009-0249-2	journal	November 2009
Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: A proposal for a synonymous codon choice that is optimal for the E. coli translational system Ikemura, Toshimichi Journal of Molecular Biology, Vol. 151, Issue 3 https://doi.org/10.1016/0022-2836(81)90003-6	journal	September 1981
Cellular concentrations of enzymes and their substrates Albe, Kathy R.; Butler, Margaret H.; Wright, Barbara E. Journal of Theoretical Biology, Vol. 143, Issue 2 https://doi.org/10.1016/S0022-5193(05)80266-8	journal	March 1990
Improved linalool production in Saccharomyces cerevisiae by combining directed evolution of linalool synthase and overexpression of the complete mevalonate pathway Zhou, Pingping; Du, Yi; Xu, Nannan Biochemical Engineering Journal, Vol. 161 https://doi.org/10.1016/j.bej.2020.107655	journal	September 2020
Metabolic engineering of yeast for fermentative production of flavonoids Rodriguez, Angelica; Strucko, Tomas; Stahlhut, Steen Gustav Bioresource Technology, Vol. 245 https://doi.org/10.1016/j.biortech.2017.06.043	journal	December 2017
Discovery and resupply of pharmacologically active plant-derived natural products: A review Atanasov, Atanas G.; Waltenberger, Birgit; Pferschy-Wenzig, Eva-Maria Biotechnology Advances, Vol. 33, Issue 8 https://doi.org/10.1016/j.biotechadv.2015.08.001	journal	December 2015
Scalable, Continuous Evolution of Genes at Mutation Rates above Genomic Error Thresholds Ravikumar, Arjun; Arzumanyan, Garri A.; Obadi, Muaeen K. A. Cell, Vol. 175, Issue 7 https://doi.org/10.1016/j.cell.2018.10.021	journal	December 2018
Full humanization of the glycolytic pathway in Saccharomyces cerevisiae Boonekamp, Francine J.; Knibbe, Ewout; Vieira-Lara, Marcel A. Cell Reports, Vol. 39, Issue 13 https://doi.org/10.1016/j.celrep.2022.111010	journal	June 2022
Molecular tools for pathway engineering in Saccharomyces cerevisiae Besada-Lombana, Pamela B.; McTaggart, Tami L.; Da Silva, Nancy A. Current Opinion in Biotechnology, Vol. 53 https://doi.org/10.1016/j.copbio.2017.12.002	journal	October 2018
High-throughput screening for efficient microbial biotechnology Sarnaik, Aditya; Liu, Arren; Nielsen, David Current Opinion in Biotechnology, Vol. 64 https://doi.org/10.1016/j.copbio.2020.02.019	journal	August 2020
Advanced Strategies for Production of Natural Products in Yeast Chen, Ruibing; Yang, Shan; Zhang, Lei iScience, Vol. 23, Issue 3 https://doi.org/10.1016/j.isci.2020.100879	journal	March 2020
K+ transport in plants: Physiology and molecular biology Szczerba, Mark W.; Britto, Dev T.; Kronzucker, Herbert J. Journal of Plant Physiology, Vol. 166, Issue 5 https://doi.org/10.1016/j.jplph.2008.12.009	journal	March 2009
Plant genome sequences: past, present, future Kersey, Paul Julian Current Opinion in Plant Biology, Vol. 48 https://doi.org/10.1016/j.pbi.2018.11.001	journal	April 2019
Codon optimization for enhanced Escherichia coli expression of human S100A11 and S100A1 proteins Marlatt, Nicole M.; Spratt, Donald E.; Shaw, Gary S. Protein Expression and Purification, Vol. 73, Issue 1 https://doi.org/10.1016/j.pep.2010.03.015	journal	September 2010
Exploiting enzyme evolution for computational protein design Pinto, Gaspar P.; Corbella, Marina; Demkiv, Andrey O. Trends in Biochemical Sciences, Vol. 47, Issue 5 https://doi.org/10.1016/j.tibs.2021.08.008	journal	May 2022
Codon bias and heterologous protein expression Gustafsson, Claes; Govindarajan, Sridhar; Minshull, Jeremy Trends in Biotechnology, Vol. 22, Issue 7 https://doi.org/10.1016/j.tibtech.2004.04.006	journal	July 2004
Production of novel antioxidative phenolic amides through heterologous expression of the plant’s chlorogenic acid biosynthesis genes in yeast Andrea, Moglia; Cinzia, Comino; Sergio, Lanteri Metabolic Engineering, Vol. 12, Issue 3 https://doi.org/10.1016/j.ymben.2009.11.003	journal	May 2010
Lipid engineering combined with systematic metabolic engineering of Saccharomyces cerevisiae for high-yield production of lycopene Ma, Tian; Shi, Bin; Ye, Ziling Metabolic Engineering, Vol. 52 https://doi.org/10.1016/j.ymben.2018.11.009	journal	March 2019
Tailored biosynthesis of gibberellin plant hormones in yeast Kildegaard, Kanchana R.; Arnesen, Jonathan A.; Adiego-Pérez, Belén Metabolic Engineering, Vol. 66 https://doi.org/10.1016/j.ymben.2021.03.010	journal	July 2021
Evolving Small-Molecule Biosensors with Improved Performance and Reprogrammed Ligand Preference Using OrthoRep Javanpour, Alex A.; Liu, Chang C. ACS Synthetic Biology, Vol. 10, Issue 10 https://doi.org/10.1021/acssynbio.1c00316	journal	October 2021
Development of a High-Throughput, In Vivo Selection Platform for NADPH-Dependent Reactions Based on Redox Balance Principles Zhang, Linyue; King, Edward; Luo, Ray ACS Synthetic Biology, Vol. 7, Issue 7 https://doi.org/10.1021/acssynbio.8b00179	journal	June 2018
AADS- An Automated Active Site Identification, Docking, and Scoring Protocol for Protein Targets Based on Physicochemical Descriptors Singh, Tanya; Biswas, D.; Jayaram, B. Journal of Chemical Information and Modeling, Vol. 51, Issue 10 https://doi.org/10.1021/ci200193z	journal	September 2011
Natural Product Synthesis Using Multicomponent Reaction Strategies Touré, Barry B.; Hall, Dennis G. Chemical Reviews, Vol. 109, Issue 9 https://doi.org/10.1021/cr800296p	journal	May 2009
The Redox Chemistry of the Covalently Immobilized Native and Low-pH Forms of Yeast Iso-1-cytochrome c Bortolotti, Carlo Augusto; Battistuzzi, Gianantonio; Borsari, Marco Journal of the American Chemical Society, Vol. 128, Issue 16 https://doi.org/10.1021/ja0573662	journal	March 2006
Global analysis of protein localization in budding yeast Huh, Won-Ki; Falvo, James V.; Gerke, Luke C. Nature, Vol. 425, Issue 6959 https://doi.org/10.1038/nature02026	journal	October 2003
High-level semi-synthetic production of the potent antimalarial artemisinin Paddon, C. J.; Westfall, P. J.; Pitera, D. J. Nature, Vol. 496, Issue 7446 https://doi.org/10.1038/nature12051	journal	April 2013
Optogenetic regulation of engineered cellular metabolism for microbial chemical production Zhao, Evan M.; Zhang, Yanfei; Mehl, Justin Nature, Vol. 555, Issue 7698 https://doi.org/10.1038/nature26141	journal	March 2018
Engineering dynamic pathway regulation using stress-response promoters Dahl, Robert H.; Zhang, Fuzhong; Alonso-Gutierrez, Jorge Nature Biotechnology, Vol. 31, Issue 11 https://doi.org/10.1038/nbt.2689	journal	October 2013
An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose DeLoache, William C.; Russ, Zachary N.; Narcross, Lauren Nature Chemical Biology, Vol. 11, Issue 7 https://doi.org/10.1038/nchembio.1816	journal	May 2015
Locating proteins in the cell using TargetP, SignalP and related tools Emanuelsson, Olof; Brunak, Søren; von Heijne, Gunnar Nature Protocols, Vol. 2, Issue 4 https://doi.org/10.1038/nprot.2007.131	journal	April 2007
Synonymous but not the same: the causes and consequences of codon bias Plotkin, Joshua B.; Kudla, Grzegorz Nature Reviews Genetics, Vol. 12, Issue 1 https://doi.org/10.1038/nrg2899	journal	November 2010
Synthesizing ginsenoside Rh2 in Saccharomyces cerevisiae cell factory at high-efficiency Wang, Pingping; Wei, Wei; Ye, Wei Cell Discovery, Vol. 5, Issue 1 https://doi.org/10.1038/s41421-018-0075-5	journal	January 2019
Synthetic biology strategies for microbial biosynthesis of plant natural products Cravens, Aaron; Payne, James; Smolke, Christina D. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-09848-w	journal	May 2019
Application of combinatorial optimization strategies in synthetic biology Naseri, Gita; Koffas, Mattheos A. G. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-16175-y	journal	May 2020
A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids Pyne, Michael E.; Kevvai, Kaspar; Grewal, Parbir S. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-17172-x	journal	July 2020
Growth-coupled selection of synthetic modules to accelerate cell factory development Orsi, Enrico; Claassens, Nico J.; Nikel, Pablo I. Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-25665-6	journal	September 2021
ECNet is an evolutionary context-integrated deep learning framework for protein engineering Luo, Yunan; Jiang, Guangde; Yu, Tianhao Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-25976-8	journal	September 2021
Strategies for microbial synthesis of high-value phytochemicals Li, Sijin; Li, Yanran; Smolke, Christina D. Nature Chemistry, Vol. 10, Issue 4 https://doi.org/10.1038/s41557-018-0013-z	journal	March 2018
Complete biosynthesis of cannabinoids and their unnatural analogues in yeast Luo, Xiaozhou; Reiter, Michael A.; d’Espaux, Leo Nature, Vol. 567, Issue 7746 https://doi.org/10.1038/s41586-019-0978-9	journal	February 2019
One thousand plant transcriptomes and the phylogenomics of green plants Nature, Vol. 574, Issue 7780, p. 679-685 https://doi.org/10.1038/s41586-019-1693-2	journal	October 2019
Biosynthesis of medicinal tropane alkaloids in yeast Srinivasan, Prashanth; Smolke, Christina D. Nature, Vol. 585, Issue 7826 https://doi.org/10.1038/s41586-020-2650-9	journal	September 2020
Highly accurate protein structure prediction with AlphaFold Jumper, John; Evans, Richard; Pritzel, Alexander Nature https://doi.org/10.1038/s41586-021-03819-2	journal	July 2021
A microbial supply chain for production of the anti-cancer drug vinblastine Zhang, Jie; Hansen, Lea G.; Gudich, Olga Nature, Vol. 609, Issue 7926 https://doi.org/10.1038/s41586-022-05157-3	journal	August 2022
Fast and accurate protein structure search with Foldseek van Kempen, Michel; Kim, Stephanie S.; Tumescheit, Charlotte Nature Biotechnology https://doi.org/10.1038/s41587-023-01773-0	journal	May 2023
Rapid generation of potent antibodies by autonomous hypermutation in yeast Wellner, Alon; McMahon, Conor; Gilman, Morgan S. A. Nature Chemical Biology, Vol. 17, Issue 10 https://doi.org/10.1038/s41589-021-00832-4	journal	June 2021
Ancestral sequence reconstruction produces thermally stable enzymes with mesophilic enzyme-like catalytic properties Furukawa, Ryutaro; Toma, Wakako; Yamazaki, Koji Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-72418-4	journal	September 2020
Codon optimization with deep learning to enhance protein expression Fu, Hongguang; Liang, Yanbing; Zhong, Xiuqin Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-74091-z	journal	October 2020
In vivo hypermutation and continuous evolution Molina, Rosana S.; Rix, Gordon; Mengiste, Amanuella A. Nature Reviews Methods Primers, Vol. 2, Issue 1 https://doi.org/10.1038/s43586-022-00119-5	journal	May 2022
Isolation and characterization of two cDNA clones encoding ATP‐sulfurylases from potato by complementation of a yeast mutant Klonus, Dirk; Höfgen, Rainer; Willmitzer, Lothar The Plant Journal, Vol. 6, Issue 1 https://doi.org/10.1046/j.1365-313X.1994.6010105.x	journal	July 1994
Translation efficiency is determined by both codon bias and folding energy Tuller, Tamir; Waldman, Yedael Y.; Kupiec, Martin Proceedings of the National Academy of Sciences, Vol. 107, Issue 8 https://doi.org/10.1073/pnas.0909910107	journal	February 2010
Complete biosynthesis of noscapine and halogenated alkaloids in yeast Li, Yanran; Li, Sijin; Thodey, Kate Proceedings of the National Academy of Sciences, Vol. 115, Issue 17 https://doi.org/10.1073/pnas.1721469115	journal	April 2018
Biological structure and function emerge from scaling unsupervised learning to 250 million protein sequences Rives, Alexander; Meier, Joshua; Sercu, Tom Proceedings of the National Academy of Sciences, Vol. 118, Issue 15 https://doi.org/10.1073/pnas.2016239118	journal	April 2021
Higher-order epistasis and phenotypic prediction Zhou, Juannan; Wong, Mandy S.; Chen, Wei-Chia Proceedings of the National Academy of Sciences, Vol. 119, Issue 39 https://doi.org/10.1073/pnas.2204233119	journal	September 2022
Vacuolar and Plasma Membrane Proton Pumps Collaborate to Achieve Cytosolic pH Homeostasis in Yeast Martínez-Muñoz, Gloria A.; Kane, Patricia Journal of Biological Chemistry, Vol. 283, Issue 29 https://doi.org/10.1074/jbc.M710470200	journal	July 2008
Jalview Version 2--a multiple sequence alignment editor and analysis workbench Waterhouse, A. M.; Procter, J. B.; Martin, D. M. A. Bioinformatics, Vol. 25, Issue 9 https://doi.org/10.1093/bioinformatics/btp033	journal	January 2009
EuGene: maximizing synthetic gene design for heterologous expression Gaspar, P.; Oliveira, J. L.; Frommlet, J. Bioinformatics, Vol. 28, Issue 20 https://doi.org/10.1093/bioinformatics/bts465	journal	July 2012
De novo biosynthesis of anthocyanins in Saccharomyces cerevisiae Eichenberger, Michael; Hansson, Anders; Fischer, David FEMS Yeast Research, Vol. 18, Issue 4 https://doi.org/10.1093/femsyr/foy046	journal	April 2018
Stromal NADH supplied by PHOSPHOGLYCERATE DEHYDROGENASE3 is crucial for photosynthetic performance Höhner, Ricarda; Day, Philip M.; Zimmermann, Sandra E. Plant Physiology, Vol. 186, Issue 1 https://doi.org/10.1093/plphys/kiaa117	journal	January 2021
Using continuous directed evolution to improve enzymes for plant applications García-García, Jorge D.; Van Gelder, Kristen; Joshi, Jaya Plant Physiology, Vol. 188, Issue 2 https://doi.org/10.1093/plphys/kiab500	journal	October 2021
Subcellular localization of the yeast proteome Kumar, A. Genes & Development, Vol. 16, Issue 6 https://doi.org/10.1101/gad.970902	journal	March 2002
GeneDesign: Rapid, automated design of multikilobase synthetic genes Richardson, S. M. Genome Research, Vol. 16, Issue 4 https://doi.org/10.1101/gr.4431306	journal	March 2006
Redox Transfer across the Inner Chloroplast Envelope Membrane Heineke, Dieter; Riens, Burgi; Grosse, Heike Plant Physiology, Vol. 95, Issue 4 https://doi.org/10.1104/pp.95.4.1131	journal	April 1991
ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites Emanuelsson, Olof; Nielsen, Henrik; Heijne, Gunnar Von Protein Science, Vol. 8, Issue 5 https://doi.org/10.1110/ps.8.5.978	journal	January 1999
Removing allosteric feedback inhibition of tomato 4-coumarate:CoA ligase by directed evolution: Directed evolution of tomato 4CL Alberstein, Moti; Eisenstein, Miriam; Abeliovich, Hagai The Plant Journal, Vol. 69, Issue 1 https://doi.org/10.1111/j.1365-313X.2011.04770.x	journal	October 2011
Advancing secondary metabolite biosynthesis in yeast with synthetic biology tools Siddiqui, Michael S.; Thodey, Kate; Trenchard, Isis FEMS Yeast Research, Vol. 12, Issue 2 https://doi.org/10.1111/j.1567-1364.2011.00774.x	journal	January 2012
Measuring enzyme activities under standardized in vivo-like conditions for systems biology: Standardized enzyme assays for systems biology van Eunen, Karen; Bouwman, Jildau; Daran-Lapujade, Pascale FEBS Journal, Vol. 277, Issue 3 https://doi.org/10.1111/j.1742-4658.2009.07524.x	journal	January 2010
A Role for the AKT1 Potassium Channel in Plant Nutrition Hirsch, R. E. Science, Vol. 280, Issue 5365 https://doi.org/10.1126/science.280.5365.918	journal	May 1998
Complete biosynthesis of opioids in yeast Galanie, S.; Thodey, K.; Trenchard, I. J. Science, Vol. 349, Issue 6252 https://doi.org/10.1126/science.aac9373	journal	August 2015
Selection on Codon Bias Hershberg, Ruth; Petrov, Dmitri A. Annual Review of Genetics, Vol. 42, Issue 1 https://doi.org/10.1146/annurev.genet.42.110807.091442	journal	December 2008
Gene Designer: a synthetic biology tool for constructing artificial DNA segments Villalobos, Alan; Ness, Jon E.; Gustafsson, Claes BMC Bioinformatics, Vol. 7, Issue 1 https://doi.org/10.1186/1471-2105-7-285	journal	June 2006
De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae Koopman, Frank; Beekwilder, Jules; Crimi, Barbara Microbial Cell Factories, Vol. 11, Issue 1 https://doi.org/10.1186/1475-2859-11-155	journal	January 2012
Expression of lignocellulolytic enzymes in Pichia pastoris Mellitzer, Andrea; Weis, Roland; Glieder, Anton Microbial Cell Factories, Vol. 11, Issue 1 https://doi.org/10.1186/1475-2859-11-61	journal	May 2012
A condition-specific codon optimization approach for improved heterologous gene expression in Saccharomyces cerevisiae Lanza, Amanda M.; Curran, Kathleen A.; Rey, Lindsey G. BMC Systems Biology, Vol. 8, Issue 1 https://doi.org/10.1186/1752-0509-8-33	journal	January 2014
High-titer production of 13R-manoyl oxide in metabolically engineered Saccharomyces cerevisiae Zhang, Chuanbo; Ju, Haiyan; Lu, Chun-Zhe Microbial Cell Factories, Vol. 18, Issue 1 https://doi.org/10.1186/s12934-019-1123-z	journal	April 2019
A code within the genetic code: codon usage regulates co-translational protein folding Liu, Yi Cell Communication and Signaling, Vol. 18, Issue 1 https://doi.org/10.1186/s12964-020-00642-6	journal	September 2020
Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme Gnanasekaran, Thiyagarajan; Vavitsas, Konstantinos; Andersen-Ranberg, Johan Journal of Biological Engineering, Vol. 9, Issue 1 https://doi.org/10.1186/s13036-015-0022-z	journal	December 2015
General Rules for Optimal Codon Choice Hershberg, Ruth; Petrov, Dmitri A. PLoS Genetics, Vol. 5, Issue 7 https://doi.org/10.1371/journal.pgen.1000556	journal	July 2009
Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans Napoli, Carolyn; Lemieux, Christine; Jorgensen, Richard The Plant Cell, Vol. 2, Issue 4 https://doi.org/10.2307/3869076	journal	April 1990
Plant Proton Pumps and Cytosolic pH-Homeostasis Cosse, Maike; Seidel, Thorsten Frontiers in Plant Science, Vol. 12 https://doi.org/10.3389/fpls.2021.672873	journal	June 2021
Comparison of Yeasts as Hosts for Recombinant Protein Production Vieira Gomes, Antonio; Souza Carmo, Talita; Silva Carvalho, Lucas Microorganisms, Vol. 6, Issue 2 https://doi.org/10.3390/microorganisms6020038	journal	April 2018

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Title: Strangers in a foreign land: ‘Yeastizing’ plant enzymes

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