Expression of dehydroshikimate dehydratase in poplar induces transcriptional and metabolic changes in the phenylpropanoid pathway

Akyuz Turumtay, Emine; Turumtay, Halbay; Tian, Yang; Lin, Chien-Yuan; Chai, Yen Ning; Louie, Katherine B.; Chen, Yan; Lipzen, Anna; Harwood, Thomas; Satish Kumar, Kavitha; Bowen, Benjamin P.; Wang, Qian; Mansfield, Shawn D.; Blow, Matthew J.; Petzold, Christopher J.; Northen, Trent R.; Mortimer, Jenny C.; Scheller, Henrik V.; Eudes, Aymerick; Zhao, ed., Qiao

doi:10.1093/jxb/erae251

Title: Expression of dehydroshikimate dehydratase in poplar induces transcriptional and metabolic changes in the phenylpropanoid pathway

Journal Article · 29 May 2024 · Journal of Experimental Botany

DOI: https://doi.org/10.1093/jxb/erae251 · OSTI ID:2438382

Akyuz Turumtay, Emine; Turumtay, Halbay;

; Lin, Chien-Yuan; Chai, Yen Ning; Louie, Katherine B.; Chen, Yan; Lipzen, Anna; Harwood, Thomas; Satish Kumar, Kavitha; Bowen, Benjamin P.; Wang, Qian;

; Blow, Matthew J.; Petzold, Christopher J.;

; Mortimer, Jenny C.;

;

; Zhao, ed., Qiao

Abstract Modification of lignin in feedstocks via genetic engineering aims to reduce biomass recalcitrance to facilitate efficient conversion processes. These improvements can be achieved by expressing exogenous enzymes that interfere with native biosynthetic pathways responsible for the production of the lignin precursors. In planta expression of a bacterial 3-dehydroshikimate dehydratase in poplar trees reduced lignin content and altered the monomer composition, which enabled higher yields of sugars after cell wall polysaccharide hydrolysis. Understanding how plants respond to such genetic modifications at the transcriptional and metabolic levels is needed to facilitate further improvement and field deployment. In this work, we acquired fundamental knowledge on lignin-modified poplar expressing 3-dehydroshikimate dehydratase using RNA-seq and metabolomics. The data clearly demonstrate that changes in gene expression and metabolite abundance can occur in a strict spatiotemporal fashion, revealing tissue-specific responses in the xylem, phloem, or periderm. In the poplar line that exhibited the strongest reduction in lignin, we found that 3% of the transcripts had altered expression levels and ~19% of the detected metabolites had differential abundance in the xylem from older stems. The changes affected predominantly the shikimate and phenylpropanoid pathways as well as secondary cell wall metabolism, and resulted in significant accumulation of hydroxybenzoates derived from protocatechuate and salicylate.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0018409

OSTI ID:: 2438382

Journal Information:: Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Journal Issue: 16 Vol. 75; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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