A potent and durable malaria transmission-blocking vaccine designed from a single-component 60-copy Pfs230D1 nanoparticle

Salinas, Nichole D.; Ma, Rui; Dickey, Thayne H.; McAleese, Holly; Ouahes, Tarik; Long, Carole A.; Miura, Kazutoyo; Lambert, Lynn E.; Tolia, Niraj H.

doi:10.1038/s41541-023-00709-8

Title: A potent and durable malaria transmission-blocking vaccine designed from a single-component 60-copy Pfs230D1 nanoparticle

Journal Article · 17 August 2023 · npj Vaccines

DOI: https://doi.org/10.1038/s41541-023-00709-8 · OSTI ID:2422630

Salinas, Nichole D. ^[1];

^[1];

^[1]; Ouahes, Tarik ^[1]; Long, Carole A. ^[2];

^[2]; Lambert, Lynn E. ^[1];

^[1]

National Institutes of Health (NIH), Bethesda, MD (United States)
National Institutes of Health (NIH), Rockville, MD (United States)

Malaria transmission-blocking vaccines (TBVs) reduce disease transmission by breaking the continuous cycle of infection between the human host and the mosquito vector. Domain 1 (D1) of Pfs230 is a leading TBV candidate and comprises the majority of transmission-reducing activity (TRA) elicited by Pfs230. Here we show that the fusion of Pfs230D1 to a 60-copy multimer of the catalytic domain of dihydrolipoyl acetyltransferase protein (E2p) results in a single-component nanoparticle composed of 60 copies of the fusion protein with high stability, homogeneity, and production yields. The nanoparticle presents a potent human transmission-blocking epitope within Pfs230D1, indicating the antigen is correctly oriented on the surface of the nanoparticle. Two vaccinations of New Zealand White rabbits with the Pfs230D1 nanoparticle elicited a potent and durable antibody response with high TRA when formulated in two distinct adjuvants suitable for translation to human use. This single-component nanoparticle vaccine may play a key role in malaria control and has the potential to improve production pipelines and the cost of manufacturing of a potent and durable TBV.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2422630

Journal Information:: npj Vaccines, Journal Name: npj Vaccines Journal Issue: 1 Vol. 8; ISSN 2059-0105

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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