Temperature modulation of CAMTA3 gene induction activity is mediated through the DNA binding domain

Chao, Lumen; Kim, Yongsig; Gilmour, Sarah J.; Thomashow, Michael F.

doi:10.1111/tpj.15944

Title: Temperature modulation of CAMTA3 gene induction activity is mediated through the DNA binding domain

Journal Article · 11 August 2022 · The Plant Journal

DOI: https://doi.org/10.1111/tpj.15944 · OSTI ID:2419371

^[1]; Kim, Yongsig ^[2]; Gilmour, Sarah J. ^[2];

^[2]

Michigan State Univ., East Lansing, MI (United States); OSTI
Michigan State Univ., East Lansing, MI (United States)

The calmodulin-binding transcription activator (CAMTA) proteins of Arabidopsis thaliana play a major role in cold acclimation, contributing to the rapid induction of the C-REPEAT BINDING FACTOR (CBF) genes and other genes that impart freezing tolerance in plants exposed to cold temperature (4°C). The goal of this study was to better understand how the gene induction activity of CAMTA3 is modulated by temperature. Our results indicate that a severely truncated version of CAMTA3, CAMTA3³³⁴, which includes the N-terminal CG-1 DNA binding domain and a newly identified transcriptional activation domain (TAD), was able to rapidly induce the expression of CBF2 and two newly identified target genes, EXPANSIN-LIKE A1 (EXPL1) and NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3), in response to cold temperature. Additionally, CAMTA3³³⁴ was able to restore freezing tolerance when expressed in a camta23 double mutant. The ability of CAMTA3 and CAMTA3³³⁴ to induce target genes at cold temperature did not involve increased levels of these proteins or increased binding of these proteins to target gene promoters in cold-treated plants. Rather, domain-swapping experiments indicated that the CAMTA3 CG-1 domain conferred temperature dependence to the ability of the CAMTA3 TAD to induce gene expression. The CG-1 domain also enabled the TAD to induce the expression of target genes at a moderate temperature (22°C) in response to cycloheximide treatment, consistent with the TAD activity not being intrinsically temperature dependent. We propose a working model in which the temperature modulation of CAMTA3 gene induction activity occurs independently from the C-terminal calmodulin-binding domains that previously have been proposed to activate CAMTA3 transcriptional activity in response to cold temperature.

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Research Organization:: Michigan State Univ., East Lansing, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 2419371

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Journal Issue: 1 Vol. 112; ISSN 0960-7412

Publisher:: Society for Experimental BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Title: Temperature modulation of CAMTA3 gene induction activity is mediated through the DNA binding domain

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