Coupling AFEX and steam-exploded sugarcane residue pellets with a room temperature CIIII-activation step lowered enzyme dosage requirements for sugar conversion

Mokomele, Thapelo; da Costa Sousa, Leonardo; Colbert, Abby; Dale, Bruce E.; Görgens, Johann F.; Balan, Venkatesh

doi:10.1016/j.cej.2022.137117

Title: Coupling AFEX and steam-exploded sugarcane residue pellets with a room temperature CIIII-activation step lowered enzyme dosage requirements for sugar conversion

Journal Article · 23 May 2022 · Chemical Engineering Journal

DOI: https://doi.org/10.1016/j.cej.2022.137117 · OSTI ID:2418898

Mokomele, Thapelo ^[1]; da Costa Sousa, Leonardo ^[2]; Colbert, Abby ^[3]; Dale, Bruce E. ^[4]; Görgens, Johann F. ^[5];

^[6]

University of Stellenbosch (South Africa); Michigan State Univ., East Lansing, MI (United States). Biomass Conversion Research Laboratory; OSTI
Michigan State Univ., East Lansing, MI (United States). Biomass Conversion Research Laboratory
Univ. of Houston, Sugarland, TX (United States)
Michigan State Univ., East Lansing, MI (United States). Biomass Conversion Research Laboratory; Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center (GLBRC)
University of Stellenbosch (South Africa)
Univ. of Houston, Sugarland, TX (United States); Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center (GLBRC)

In this study, the potential integration of steam explosion (StEx) and ammonia fiber expansion (AFEX) with existing sugar/ethanol mills to form decentralized pre-processing depots was explored. Both StEx and AFEX pretreatment facilitated the production of sugarcane bagasse (SCB) and cane leaf matter (CLM) pellets with significantly higher bulk density, mechanical durability, and hydrophobicity relative to their untreated biomass pellet controls. However, ethanol production from standalone StEx and AFEX-treated SCB and CLM pellets required enzyme dosages greater than 21 mg/g glucan to achieve enzymatic hydrolysis sugar yields of 75% and ethanol titres greater than 40 g.L^-1. Coupling AFEX-treated SCB or CLM pellets with a room temperature CIII_I-activation step using liquid ammonia lowered enzyme dosage requirements by more than 50% without affecting ethanol titers and production yields (greater than300 L per Mg residual dry matter raw dry biomass (RDM)). In contrast, treating StEx-treated pellets with CIIII-activation using liquid ammonia did not result in similar enzyme dosage reductions, due to pseudo-lignin formation, leading to enzyme deactivation and/or lignin blockage that retarded enzymatic hydrolysis at low enzyme dosages. A gross energy conversion assessment revealed that low enzyme dosage (3-4 mg enzyme/g RDM) ethanol and electricity co-production from AFEX and CIII_I-activated SCB and CLM can recover up to 73% of the energy in the untreated biomass, compared to 54% recovered by StEx and CIII_I-activation. The results from this work suggest that StEx or AFEX based pre-processing depots can produce dense and mechanically durable biomass pellets. The AFEX-treated pellets can be easily upgraded using a room temperature CIII_I-activation step at the biorefinery to significantly reduce bioconversion enzymes.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 2418898

Journal Information:: Chemical Engineering Journal, Journal Name: Chemical Engineering Journal Journal Issue: P2 Vol. 446; ISSN 1385-8947

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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42 ENGINEERING
AFEX
Engineering
Enzyme dosage
Ethanol
Steam explosion
Sugarcane residues
Uniform feedstock supply

Title: Coupling AFEX and steam-exploded sugarcane residue pellets with a room temperature CIIII-activation step lowered enzyme dosage requirements for sugar conversion

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