In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum

Abhange, Komal; Kitata, Reta Birhanu; Zhang, Jie; Wang, Yi-Ting; Gaffrey, Matthew J.; Liu, Tao; Gunchick, Valerie; Khaykin, Valerie; Sahai, Vaibhav; Cuneo, Kyle C.; Parikh, Neehar D.; Shi, Tujin; Lubman, David M.

doi:10.1021/acs.jproteome.3c00614

Title: In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum

Journal Article · 19 December 2023 · Journal of Proteome Research

DOI: https://doi.org/10.1021/acs.jproteome.3c00614 · OSTI ID:2395916

^[1];

^[2]; Zhang, Jie ^[1]; Wang, Yi-Ting ^[2]; Gaffrey, Matthew J. ^[2];

^[2]; Gunchick, Valerie ^[3];

^[3]; Sahai, Vaibhav ^[3]; Cuneo, Kyle C. ^[3]; Parikh, Neehar D. ^[3];

^[2];

^[1]

University of Michigan Medical Center, Ann Arbor, MI (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Univ. of Michigan, Ann Arbor, MI (United States)

Extracellular Vesicle (EV) secretion has been observed from most types of normal and tumor cells. These EVs contain a variety of distinctive cargo where tumor-derived serum proteins in EVs secreted into the bloodstream can be used to provide a minimally invasive method for clinical monitoring. We have therefore undertaken a comprehensive study of the protein content of the EVs from several cancer cell lines using direct Data Independent Analysis (DIA). Further, several thousand proteins were detected including many of the classic EV markers such as CD9, CD81, CD63, TSG101 and Syndecan-1 among others. Many distinctive cancer related proteins were detected and some of them are known to be potential markers of cancer detection and monitoring of cancer progression. We have further studied the protein content of EVs from patient serum for both normal controls and pancreatic (PC) and Hepatocellular Carcinoma (HCC) cancers. The EVs for these studies have been isolated by various methods for comparison including ultracentrifugation (UC) and CD9 immunoaffinity column (CD9-IC). Typically, 500-1,000 proteins were identified where most of them overlapped with the EV proteins identified from the cell lines studied. Many of the EV protein markers were identified in these serum EVs and large numbers of proteins related to pancreatic and HCC cancers were identified.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE; National Cancer Institute (NCI); National Institutes of Health (NIH)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 2395916

Report Number(s):: PNNL-SA--190106

Journal Information:: Journal of Proteome Research, Journal Name: Journal of Proteome Research Journal Issue: 1 Vol. 23; ISSN 1535-3893

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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60 APPLIED LIFE SCIENCES
CD9
EV proteins
HCC
extracellular vesicles
immunoaffinity
pancreatic cancer
ultracentrifugation

Title: In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum

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References (46)

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