Assessing microbial systems and process configurations for improved ethanol production from sugary stovers by integrating soluble sugars and holocellulose

Zabed, Hossain M.; Akter, Suely; Tian, Yuehui; Dar, Mudasir A.; Yun, Junhua; Zhao, Mei; Ragauskas, Arthur J.; Li, Jia; Qi, Xianghui

doi:10.1016/j.indcrop.2024.118269

Title: Assessing microbial systems and process configurations for improved ethanol production from sugary stovers by integrating soluble sugars and holocellulose

Journal Article · 02 March 2024 · Industrial Crops and Products

DOI: https://doi.org/10.1016/j.indcrop.2024.118269 · OSTI ID:2376325

Zabed, Hossain M. ^[1]; Akter, Suely ^[2]; Tian, Yuehui ^[1]; Dar, Mudasir A. ^[2]; Yun, Junhua ^[1]; Zhao, Mei ^[2];

^[3]; Li, Jia ^[1]; Qi, Xianghui ^[4]

Guangzhou University (China)
Jiangsu University (China)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)
Guangzhou University (China); Jiangsu University (China)

Here, this study evaluated microbial systems and technological approaches to configure the whole slurry co-fermentation process for ethanol biosynthesis from a novel stover system rich in sugars. Two approaches, namely separate and simultaneous hydrolysis and co-fermentation (SHCF and SSCF, respectively), were investigated using Escherichia coli monoculture and E. coli-yeast coculture. The SSCF with E. coli monoculture produced 32.75 g/L ethanol, representing only 44.87% yield, which left 65.13 g/L of total sugars unconverted and exhibited limited xylose consumption. Subsequently, a coculture-based SHCF significantly enhanced sugar consumption, leading to increase in ethanol yield and concentration to 66.94% and 48.86 g/L, respectively. Nevertheless, xylose utilization remained minimal due to the preference for glucose and the inhibitory effects of certain compounds. Thereafter, modification of the medium composition by supplementing betaine and sodium metabisulfite improved ethanol production to 53.18 g/L by reducing the toxic effects of inhibitors. Finally, a dual-phase SSCF (DP-SSCF) was explored by allowing the consumption of sugars from the pretreated slurry in the first phase, followed by concurrent cellulose hydrolysis and utilization of the resulting glucose in the second phase. This strategy increased ethanol titer to 63.14 g/L, with 84.3% yield and 0.88 g/L/h productivity.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 2376325

Journal Information:: Industrial Crops and Products, Journal Name: Industrial Crops and Products Vol. 212; ISSN 0926-6690

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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09 BIOMASS FUELS
Bioethanol
Bioprocessing
Biorefinery
Corn stover
Microbial coculture

Title: Assessing microbial systems and process configurations for improved ethanol production from sugary stovers by integrating soluble sugars and holocellulose

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