Nanoscopic lipid domains determined by microscopy and neutron scattering

Collier, Charles Patrick; Bolmatov, Dima; Elkins, James G.; Katsaras, John

doi:10.1016/j.ymeth.2024.01.020

Title: Nanoscopic lipid domains determined by microscopy and neutron scattering

Journal Article · 05 February 2024 · Methods

DOI: https://doi.org/10.1016/j.ymeth.2024.01.020 · OSTI ID:2376309

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Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Shull Wollan Center
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Biological membranes are highly complex supramolecular assemblies, which play central roles in biology. However, their complexity makes them challenging to study their nanoscale structures. To overcome this challenge, model membranes assembled using reduced sets of membrane-associated biomolecules have been found to be both excellent and tractable proxies for biological membranes. Due to their relative simplicity, they have been studied using a range of biophysical characterization techniques. Here, in this review article, we will briefly detail the use of fluorescence and electron microscopies, and X-ray and neutron scattering techniques used over the past few decades to study the nanostructure of biological membranes.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 2376309

Alternate ID(s):: OSTI ID: 2301780

Journal Information:: Methods, Journal Name: Methods Vol. 223; ISSN 1046-2023

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Title: Nanoscopic lipid domains determined by microscopy and neutron scattering

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