Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

Gomez, Miguel; Leung, Whinkie; Dantuluri, Swathi; Pillai, Alexander; Gani, Zyan; Hwang, Sungmin; McMillan, Lana J.; Kiljunen, Saija; Savilahti, Harri; Maupin-Furlow, Julie A.

doi:10.3390/genes9110562

Title: Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

Journal Article · 19 November 2018 · Genes

DOI: https://doi.org/10.3390/genes9110562 · OSTI ID:2372727

Gomez, Miguel; Leung, Whinkie; Dantuluri, Swathi; Pillai, Alexander; Gani, Zyan; Hwang, Sungmin; McMillan, Lana J.;

; Savilahti, Harri;

Halophilic archaea thrive in hypersaline conditions associated with desiccation, ultraviolet (UV) irradiation and redox active compounds, and thus are naturally tolerant to a variety of stresses. Here, we identified mutations that promote enhanced tolerance of halophilic archaea to redox-active compounds using Haloferax volcanii as a model organism. The strains were isolated from a library of random transposon mutants for growth on high doses of sodium hypochlorite (NaOCl), an agent that forms hypochlorous acid (HOCl) and other redox acid compounds common to aqueous environments of high concentrations of chloride. The transposon insertion site in each of twenty isolated clones was mapped using the following: (i) inverse nested two-step PCR (INT-PCR) and (ii) semi-random two-step PCR (ST-PCR). Genes that were found to be disrupted in hypertolerant strains were associated with lysine deacetylation, proteasomes, transporters, polyamine biosynthesis, electron transfer, and other cellular processes. Further analysis revealed a ΔpsmA1 (α1) markerless deletion strain that produces only the α2 and β proteins of 20S proteasomes was hypertolerant to hypochlorite stress compared with wild type, which produces α1, α2, and β proteins. The results of this study provide new insights into archaeal tolerance of redox active compounds such as hypochlorite.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: FG02-05ER15650

OSTI ID:: 2372727

Journal Information:: Genes, Journal Name: Genes Journal Issue: 11 Vol. 9; ISSN 2073-4425; ISSN GENEG9

Publisher:: MDPI AGCopyright Statement

Country of Publication:: Switzerland

Language:: English

References (74)

Rapid Amplification of Uncharacterized Transposon-tagged DNA Sequences from Genomic DNA Chun, Kristin T.; Edenberg, Howard J.; Kelley, Mark R. Yeast, Vol. 13, Issue 3 https://doi.org/10.1002/(SICI)1097-0061(19970315)13:3<233::AID-YEA88>3.0.CO;2-E	journal	March 1997
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Crystal structure of TTHA1429, a novel metallo-β-lactamase superfamily protein from Thermus thermophilus HB8 Yamamura, Akihiro; Ohtsuka, Jun; Kubota, Keiko Proteins: Structure, Function, and Bioinformatics, Vol. 73, Issue 4 https://doi.org/10.1002/prot.22215	journal	September 2008
Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen Krogh, Anders; Larsson, Björn; von Heijne, Gunnar Journal of Molecular Biology, Vol. 305, Issue 3 https://doi.org/10.1006/jmbi.2000.4315	journal	January 2001
Transposon Insertion Mutagenesis for Archaeal Gene Discovery Kiljunen, Saija; Pajunen, Maria I.; Savilahti, Harri Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-6472-7_20	book	October 2016
Predicting Secretory Proteins with SignalP Nielsen, Henrik Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-7015-5_6	book	January 2017
Halobacterium volcanii spec. nov., a Dead Sea halobacterium with a moderate salt requirement Mullakhanbhai, Moiz F.; Larsen, Helge Archives of Microbiology, Vol. 104, Issue 1 https://doi.org/10.1007/BF00447326	journal	January 1975
The microbiology and biogeochemistry of the Dead Sea Nissenbaum, Arie Microbial Ecology, Vol. 2, Issue 2 https://doi.org/10.1007/BF02010435	journal	June 1975
Polyamines: fundamental characters in chemistry and biology Agostinelli, E.; Marques, M. P. M.; Calheiros, R. Amino Acids, Vol. 38, Issue 2 https://doi.org/10.1007/s00726-009-0396-7	journal	December 2009
The replacement of the nondescript term ‘heavy metals’ by a biologically and chemically significant classification of metal ions Nieboer, Evert; Richardson, David H. S. Environmental Pollution Series B, Chemical and Physical, Vol. 1, Issue 1 https://doi.org/10.1016/0143-148X(80)90017-8	journal	January 1980
The primary structure of halocyanin, an archaeal blue copper protein, predicts a lipid anchor for membrane fixation. Mattar, S.; Scharf, B.; Kent, S. B. Journal of Biological Chemistry, Vol. 269, Issue 21 https://doi.org/10.1016/S0021-9258(17)36556-0	journal	May 1994
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
An in vivo system involving co-expression of cyanobacterial flavodoxin and ferredoxin-NADP ⁺ reductase confers increased tolerance to oxidative stress in plants Giró, Mariana; Ceccoli, Romina D.; Poli, Hugo O. FEBS Open Bio, Vol. 1, Issue 1 https://doi.org/10.1016/j.fob.2011.10.004	journal	November 2011
The Oligomeric Assembly of the Novel Haem-Degrading Protein HbpS Is Essential for Interaction with Its Cognate Two-Component Sensor Kinase de Orué Lucana, Darío Ortiz; Bogel, Gabriele; Zou, Peijian Journal of Molecular Biology, Vol. 386, Issue 4 https://doi.org/10.1016/j.jmb.2009.01.017	journal	March 2009
Crystal Structures and Enzymatic Properties of a Triamine/Agmatine Aminopropyltransferase from Thermus thermophilus Ohnuma, Mio; Ganbe, Tadashi; Terui, Yusuke Journal of Molecular Biology, Vol. 408, Issue 5 https://doi.org/10.1016/j.jmb.2011.03.025	journal	May 2011
Maintaining a Healthy Proteome during Oxidative Stress Reichmann, Dana; Voth, Wilhelm; Jakob, Ursula Molecular Cell, Vol. 69, Issue 2 https://doi.org/10.1016/j.molcel.2017.12.021	journal	January 2018
Systematic Analysis of Lysine Acetylation in the Halophilic Archaeon Haloferax mediterranei Liu, Jingfang; Wang, Qian; Jiang, Xiongjian Journal of Proteome Research, Vol. 16, Issue 9 https://doi.org/10.1021/acs.jproteome.7b00222	journal	August 2017
Free Radical Oxidation of Polyunsaturated Lipids: New Mechanistic Insights and the Development of Peroxyl Radical Clocks Pratt, Derek A.; Tallman, Keri A.; Porter, Ned A. Accounts of Chemical Research, Vol. 44, Issue 6 https://doi.org/10.1021/ar200024c	journal	April 2011
Electron Transfer Proteins from the Haloalkaliphilic Archaeon Natronobacterium pharaonis: Possible Components of the Respiratory Chain Include Cytochrome bc and a Terminal Oxidase Cytochrome ba₃ Scharf, Birgit; Wittenberg, Roswitha; Engelhard, Martin Biochemistry, Vol. 36, Issue 15 https://doi.org/10.1021/bi962312d	journal	April 1997
Shotgun Proteomics of the Haloarchaeon Haloferax volcanii Kirkland, P. Aaron; Humbard, Matthew A.; Daniels, Charles J. Journal of Proteome Research, Vol. 7, Issue 11 https://doi.org/10.1021/pr800517a	journal	November 2008
The Phyre2 web portal for protein modeling, prediction and analysis Kelley, Lawrence A.; Mezulis, Stefans; Yates, Christopher M. Nature Protocols, Vol. 10, Issue 6 https://doi.org/10.1038/nprot.2015.053	journal	May 2015
A mechanism for intracellular release of Na+ by neurotransmitter/sodium symporters Malinauskaite, Lina; Quick, Matthias; Reinhard, Linda Nature Structural & Molecular Biology, Vol. 21, Issue 11 https://doi.org/10.1038/nsmb.2894	journal	October 2014
‘Unknown’ proteins and ‘orphan’ enzymes: the missing half of the engineering parts list – and how to find it Hanson, Andrew D.; Pribat, Anne; Waller, Jeffrey C. Biochemical Journal, Vol. 425, Issue 1 https://doi.org/10.1042/BJ20091328	journal	December 2009
Adaptation of protein secretion to extremely high-salt conditions by extensive use of the twin-arginine translocation pathway Rose, R. Wesley; Bruser, Thomas; Kissinger, Jessica C. Molecular Microbiology, Vol. 45, Issue 4 https://doi.org/10.1046/j.1365-2958.2002.03090.x	journal	August 2002
The housekeeping dipeptide permease is the Escherichia coli heme transporter and functions with two optional peptide binding proteins Letoffe, S.; Delepelaire, P.; Wandersman, C. Proceedings of the National Academy of Sciences, Vol. 103, Issue 34 https://doi.org/10.1073/pnas.0605440103	journal	August 2006
E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea Miranda, H. V.; Nembhard, N.; Su, D. Proceedings of the National Academy of Sciences, Vol. 108, Issue 11 https://doi.org/10.1073/pnas.1018151108	journal	February 2011
NemR Is a Bleach-sensing Transcription Factor Gray, Michael J.; Wholey, Wei-Yun; Parker, Benjamin W. Journal of Biological Chemistry, Vol. 288, Issue 19 https://doi.org/10.1074/jbc.M113.454421	journal	May 2013
Crystal Structure of a PhoU Protein Homologue: A NEW CLASS OF METALLOPROTEIN CONTAINING MULTINUCLEAR IRON CLUSTERS Liu, Jinyu; Lou, Yun; Yokota, Hisao Journal of Biological Chemistry, Vol. 280, Issue 16 https://doi.org/10.1074/jbc.M414117200	journal	February 2005
The Mismetallation of Enzymes during Oxidative Stress Imlay, James A. Journal of Biological Chemistry, Vol. 289, Issue 41 https://doi.org/10.1074/jbc.R114.588814	journal	August 2014
Identification of a novel acetylated form of branched-chain polyamine from a hyperthermophilic archaeon Thermococcus kodakarensis Hidese, Ryota; Im, Ki-Hwan; Kobayashi, Masaki Bioscience, Biotechnology, and Biochemistry, Vol. 81, Issue 9 https://doi.org/10.1080/09168451.2017.1345616	journal	September 2017
Free Radicals in Biology: Oxidative Stress and the Effects of Ionizing Radiation Riley, P. A. International Journal of Radiation Biology, Vol. 65, Issue 1 https://doi.org/10.1080/09553009414550041	journal	January 1994
PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes Yu, Nancy Y.; Wagner, James R.; Laird, Matthew R. Bioinformatics, Vol. 26, Issue 13 https://doi.org/10.1093/bioinformatics/btq249	journal	May 2010
Protter: interactive protein feature visualization and integration with experimental proteomic data Omasits, Ulrich; Ahrens, Christian H.; Müller, Sebastian Bioinformatics, Vol. 30, Issue 6 https://doi.org/10.1093/bioinformatics/btt607	journal	October 2013
An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications Haapa, S.; Taira, S.; Heikkinen, E. Nucleic Acids Research, Vol. 27, Issue 13 https://doi.org/10.1093/nar/27.13.2777	journal	July 1999
The UCSC Archaeal Genome Browser Schneider, K. L. Nucleic Acids Research, Vol. 34, Issue 90001 https://doi.org/10.1093/nar/gkj134	journal	January 2006
InterPro in 2017—beyond protein family and domain annotations Finn, Robert D.; Attwood, Teresa K.; Babbitt, Patricia C. Nucleic Acids Research, Vol. 45, Issue D1 https://doi.org/10.1093/nar/gkw1107	journal	November 2016
A new simvastatin (mevinolin)-resistance marker from Haloarcula hispanica and a new Haloferax volcanii strain cured of plasmid pHV2 Dyall-Smith, M. L.; Wendoloski, D.; Ferrer, C. Microbiology, Vol. 147, Issue 4 https://doi.org/10.1099/00221287-147-4-959	journal	April 2001
Multiplex quantitative SILAC for analysis of archaeal proteomes: a case study of oxidative stress responses: Multiplex SILAC for archaeal proteomics McMillan, Lana J.; Hwang, Sungmin; Farah, Rawan E. Environmental Microbiology, Vol. 20, Issue 1 https://doi.org/10.1111/1462-2920.14014	journal	December 2017
GlyoxalaseIreduces glycative and oxidative stress and prevents age‐related endothelial dysfunction through modulation of endothelial nitric oxide synthase phosphorylation Jo‐Watanabe, Airi; Ohse, Takamoto; Nishimatsu, Hiroaki Aging Cell, Vol. 13, Issue 3 https://doi.org/10.1111/acel.12204	journal	February 2014
Chloride flux in phagocytes Wang, Guoshun Immunological Reviews, Vol. 273, Issue 1 https://doi.org/10.1111/imr.12438	journal	August 2016
Haloferax volcanii twin-arginine translocation substates include secreted soluble, C-terminally anchored and lipoproteins Giménez, María I.; Dilks, Kieran; Pohlschröder, Mechthild Molecular Microbiology, Vol. 66, Issue 6 https://doi.org/10.1111/j.1365-2958.2007.06034.x	journal	November 2007
Regulation of phosphate uptake via Pst transporters inHalobacterium salinarumR1 Furtwängler, Katarina; Tarasov, Valery; Wende, Andy Molecular Microbiology, Vol. 76, Issue 2 https://doi.org/10.1111/j.1365-2958.2010.07101.x	journal	April 2010
The BCCT family of carriers: from physiology to crystal structure: BCCT carriers Ziegler, Christine; Bremer, Erhard; Krämer, Reinhard Molecular Microbiology https://doi.org/10.1111/j.1365-2958.2010.07332.x	journal	September 2010
Efflux of choline and glycine betaine from osmoregulating cells ofEscherichia coli Lamark, Trond; Styrvold, Olaf B.; StrÃ¸m, Arne R. FEMS Microbiology Letters, Vol. 96, Issue 2-3 https://doi.org/10.1111/j.1574-6968.1992.tb05408.x	journal	September 1992
Physiological polyamines: simple primordial stress molecules Rhee, H. J.; Kim, Eui-Jin; Lee, J. K. Journal of Cellular and Molecular Medicine, Vol. 11, Issue 4 https://doi.org/10.1111/j.1582-4934.2007.00077.x	journal	July 2007
The insand outsof bacterial iron metabolism Frawley, Elaine R.; Fang, Ferric C. Molecular Microbiology, Vol. 93, Issue 4 https://doi.org/10.1111/mmi.12709	journal	July 2014
The Interaction of Alba, a Conserved Archaeal Chromatin Protein, with Sir2 and Its Regulation by Acetylation Bell, S. D. Science, Vol. 296, Issue 5565 https://doi.org/10.1126/science.1070506	journal	April 2002
Chlorination and Cleavage of Lignin Structures by Fungal Chloroperoxidases Ortiz-Bermudez, P.; Srebotnik, E.; Hammel, K. E. Applied and Environmental Microbiology, Vol. 69, Issue 8 https://doi.org/10.1128/AEM.69.8.5015-5018.2003	journal	August 2003
Development of Additional Selectable Markers for the Halophilic Archaeon Haloferax volcanii Based on the leuB and trpA Genes Allers, T.; Ngo, H. -P.; Mevarech, M. Applied and Environmental Microbiology, Vol. 70, Issue 2 https://doi.org/10.1128/AEM.70.2.943-953.2004	journal	February 2004
Glycerol-Mediated Repression of Glucose Metabolism and Glycerol Kinase as the Sole Route of Glycerol Catabolism in the Haloarchaeon Haloferax volcanii Sherwood, K. E.; Cano, D. J.; Maupin-Furlow, J. A. Journal of Bacteriology, Vol. 191, Issue 13 https://doi.org/10.1128/JB.00131-09	journal	May 2009
PhoU2 but Not PhoU1 as an Important Regulator of Biofilm Formation and Tolerance to Multiple Stresses by Participating in Various Fundamental Metabolic Processes in Staphylococcus epidermidis Wang, Xiaofei; Han, Haiyan; Lv, Zhihui Journal of Bacteriology, Vol. 199, Issue 24 https://doi.org/10.1128/JB.00219-17	journal	December 2017
Proteasomal Components Required for Cell Growth and Stress Responses in the Haloarchaeon Haloferax volcanii Zhou, G.; Kowalczyk, D.; Humbard, M. A. Journal of Bacteriology, Vol. 190, Issue 24 https://doi.org/10.1128/JB.01180-08	journal	October 2008
Genetic Evidence for the Importance of Protein Acetylation and Protein Deacetylation in the Halophilic Archaeon Haloferax volcanii Altman-Price, N.; Mevarech, M. Journal of Bacteriology, Vol. 191, Issue 5 https://doi.org/10.1128/JB.01252-08	journal	December 2008
Halophilic 20S Proteasomes of the ArchaeonHaloferax volcanii: Purification, Characterization, and Gene Sequence Analysis Wilson, Heather L.; Aldrich, Henry C.; Maupin-Furlow, Julie Journal of Bacteriology, Vol. 181, Issue 18 https://doi.org/10.1128/JB.181.18.5814-5824.1999	journal	September 1999
Cytochrome aa ₃ in Haloferax volcanii Tanaka, Mikiei; Ogawa, Naohide; Ihara, Kunio Journal of Bacteriology, Vol. 184, Issue 3 https://doi.org/10.1128/JB.184.3.840-845.2002	journal	February 2002
Subunit Topology of Two 20S Proteasomes from Haloferax volcanii Kaczowka, Steven J.; Maupin-Furlow, Julie A. Journal of Bacteriology, Vol. 185, Issue 1 https://doi.org/10.1128/JB.185.1.165-174.2003	journal	January 2003
Differential Regulation of the PanA and PanB Proteasome-Activating Nucleotidase and 20S Proteasomal Proteins of the Haloarchaeon Haloferax volcanii Reuter, Christopher J.; Kaczowka, Steven J.; Maupin-Furlow, Julie A. Journal of Bacteriology, Vol. 186, Issue 22 https://doi.org/10.1128/JB.186.22.7763-7772.2004	journal	November 2004
Ubiquitin-Like Proteasome System Represents a Eukaryotic-Like Pathway for Targeted Proteolysis in Archaea Fu, Xian; Liu, Rui; Sanchez, Iona mBio, Vol. 7, Issue 3 https://doi.org/10.1128/mBio.00379-16	journal	May 2016
Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria Tam, R.; Saier, M. H. Microbiological Reviews, Vol. 57, Issue 2 https://doi.org/10.1128/mr.57.2.320-346.1993	journal	June 1993
Hypochlorous acid as a precursor of free radicals in living systems Panasenko, O. M.; Gorudko, I. V.; Sokolov, A. V. Biochemistry (Moscow), Vol. 78, Issue 13 https://doi.org/10.1134/S0006297913130075	journal	December 2013
REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal Transduction Apel, Klaus; Hirt, Heribert Annual Review of Plant Biology, Vol. 55, Issue 1 https://doi.org/10.1146/annurev.arplant.55.031903.141701	journal	June 2004
Deciphering the Translation Initiation Factor 5A Modification Pathway in Halophilic Archaea Prunetti, Laurence; Graf, Michael; Blaby, Ian K. Archaea, Vol. 2016 https://doi.org/10.1155/2016/7316725	journal	January 2016
SyntTax: a web server linking synteny to prokaryotic taxonomy Oberto, Jacques BMC Bioinformatics, Vol. 14, Issue 1 https://doi.org/10.1186/1471-2105-14-4	journal	January 2013
Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery Kiljunen, Saija; Pajunen, Maria I.; Dilks, Kieran BMC Biology, Vol. 12, Issue 1 https://doi.org/10.1186/s12915-014-0103-3	journal	December 2014
Mre11-Rad50 Promotes Rapid Repair of DNA Damage in the Polyploid Archaeon Haloferax volcanii by Restraining Homologous Recombination Delmas, Stéphane; Shunburne, Lee; Ngo, Hien-Ping PLoS Genetics, Vol. 5, Issue 7 https://doi.org/10.1371/journal.pgen.1000552	journal	July 2009
The Complete Genome Sequence of Haloferax volcanii DS2, a Model Archaeon Hartman, Amber L.; Norais, Cédric; Badger, Jonathan H. PLoS ONE, Vol. 5, Issue 3 https://doi.org/10.1371/journal.pone.0009605	journal	March 2010
CLCAs - A Family of Metalloproteases of Intriguing Phylogenetic Distribution and with Cases of Substituted Catalytic Sites Lenart, Anna; Dudkiewicz, Małgorzata; Grynberg, Marcin PLoS ONE, Vol. 8, Issue 5 https://doi.org/10.1371/journal.pone.0062272	journal	May 2013
Fenton Chemistry: An Introduction Wardman, Peter; Candeias, Luis P. Radiation Research, Vol. 145, Issue 5 https://doi.org/10.2307/3579270	journal	May 1996
Ubiquitous occurrence of agmatine as the major polyamine within extremely halophilic archaebacteria. Hamana, Koei; Hamana, Hiroshi; Itoh, Takashi The Journal of General and Applied Microbiology, Vol. 41, Issue 2 https://doi.org/10.2323/jgam.41.153	journal	January 1995
Iron, copper, zinc, and manganese transport and regulation in pathogenic Enterobacteria: correlations between strains, site of infection and the relative importance of the different metal transport systems for virulence Porcheron, Gaëlle; Garénaux, Amélie; Proulx, Julie Frontiers in Cellular and Infection Microbiology, Vol. 3 https://doi.org/10.3389/fcimb.2013.00090	journal	January 2013
Redox regulation by reversible protein S-thiolation in bacteria Loi, Vu Van; Rossius, Martina; Antelmann, Haike Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.00187	journal	March 2015
DNA Repair and Photoprotection: Mechanisms of Overcoming Environmental Ultraviolet Radiation Exposure in Halophilic Archaea Jones, Daniel L.; Baxter, Bonnie K. Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.01882	journal	September 2017
A Review of Antioxidant Peptides Derived from Meat Muscle and By-Products Liu, Rui; Xing, Lujuan; Fu, Qingquan Antioxidants, Vol. 5, Issue 3 https://doi.org/10.3390/antiox5030032	journal	September 2016
Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants Legerme, Georgio; Yang, Evan; Esquivel, Rianne Life, Vol. 6, Issue 4 https://doi.org/10.3390/life6040041	journal	November 2016

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59 BASIC BIOLOGICAL SCIENCES
Genetics & Heredity
archaea
hypochlorite
oxidative stress
proteasome
redox-active

Title: Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

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