Advanced manufacturing provides tailor-made solutions for crystallography with x-ray free-electron lasers

Paulson, Lars; Narayanasamy, Sankar Raju; Shelby, Megan L.; Frank, Matthias; Trebbin, Martin

doi:10.1063/4.0000229

Title: Advanced manufacturing provides tailor-made solutions for crystallography with x-ray free-electron lasers

Journal Article · 20 February 2024 · Structural Dynamics

DOI: https://doi.org/10.1063/4.0000229 · OSTI ID:2370620

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^[2]; Shelby, Megan L. ^[2];

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State University of New York at Buffalo, NY (United States)
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States); University of California, Davis, CA (United States)
State University of New York at Buffalo, NY (United States); Hauptman-Woodward Medical Research Institute, Buffalo, NY (United States)

Serial crystallography at large facilities, such as x-ray free-electron lasers and synchrotrons, evolved as a powerful method for the high-resolution structural investigation of proteins that are critical for human health, thus advancing drug discovery and novel therapies. However, a critical barrier to successful serial crystallography experiments lies in the efficient handling of the protein microcrystals and solutions at microscales. Microfluidics are the obvious approach for any high-throughput, nano-to-microliter sample handling, that also requires design flexibility and rapid prototyping to deal with the variable shapes, sizes, and density of crystals. Here, we discuss recent advances in polymer 3D printing for microfluidics-based serial crystallography research and present a demonstration of emerging, large-scale, nano-3D printing approaches leading into the future of 3D sample environment and delivery device fabrication from liquid jet gas-dynamic virtual nozzles devices to fixed-target sample environment technology.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); National Institute of Health (NIH)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 2370620

Report Number(s):: LLNL--JRNL-856279; 1085904

Journal Information:: Structural Dynamics, Journal Name: Structural Dynamics Journal Issue: 1 Vol. 11; ISSN 2329-7778

Publisher:: American Crystallographic Association/AIPCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

36 MATERIALS SCIENCE
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
3D printing
42 ENGINEERING
59 BASIC BIOLOGICAL SCIENCES
77 NANOSCIENCE AND NANOTECHNOLOGY
crystallography
fluid jets
free electron lasers
microfluidics
multiphoton lithography
proteins
sample handling
synchrotrons
therapeutics

Title: Advanced manufacturing provides tailor-made solutions for crystallography with x-ray free-electron lasers

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