Engineered poplar for bioproduction of the triterpene squalene

Bibik, Jacob D.; Sahu, Abira; Kim, Boeun; Unda, Faride; Andersen, Trine B.; Mansfield, Shawn D.; Maravelias, Christos T.; Sharkey, Thomas D.; Hamberger, Björn R.

doi:10.1111/pbi.14345

Title: Engineered poplar for bioproduction of the triterpene squalene

Journal Article · 20 March 2024 · Plant Biotechnology Journal

DOI: https://doi.org/10.1111/pbi.14345 · OSTI ID:2370307

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Michigan State University, East Lansing, MI (United States); Michigan State University Department of Biochemistry & Molecular Biology
Michigan State University, East Lansing, MI (United States)
Michigan State University, East Lansing, MI (United States); Princeton University, NJ (United States)
Michigan State University, East Lansing, MI (United States); University of British Columbia, Vancouver, BC (Canada)
Princeton University, NJ (United States)

Building sustainable platforms to produce biofuels and specialty chemicals has become an increasingly important strategy to supplement and replace fossil fuels and petrochemical-derived products. Terpenoids are the most diverse class of natural products that have many commercial roles as specialty chemicals. Poplar is a fast growing, biomassdense bioenergy crop with many species known to produce large amounts of the hemiterpene isoprene, suggesting an inherent capacity to produce significant quantities of other terpenes. Here we aimed to engineer poplar with optimized pathways to produce squalene, a triterpene commonly used in cosmetic oils, a potential biofuel candidate, and the precursor to the further diversified classes of triterpenoids and sterols. The squalene production pathways were either re-targeted from the cytosol to plastids or co-produced with lipid droplets in the cytosol. Squalene and lipid droplet co-production appeared to be toxic, which we hypothesize to be due to disruption of adventitious root formation, suggesting a need for tissue specific production. Plastidial squalene production enabled up to 0.63 mg/g fresh weight in leaf tissue, which also resulted in reductions in isoprene emission and photosynthesis. These results were also studied through a technoeconomic analysis, providing further insight into developing poplar as a production host.

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Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409

OSTI ID:: 2370307

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Journal Issue: 8 Vol. 22; ISSN 1467-7644

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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