Mixed waste contamination selects for a mobile genetic element population enriched in multiple heavy metal resistance genes

Goff, Jennifer L.; Lui, Lauren M.; Nielsen, Torben N.; Poole, Farris L.; Smith, Heidi J.; Walker, Kathleen F.; Hazen, Terry C.; Fields, Matthew W.; Arkin, Adam P.; Adams, Michael W. W.

doi:10.1093/ismeco/ycae064

Title: Mixed waste contamination selects for a mobile genetic element population enriched in multiple heavy metal resistance genes

Journal Article · 08 May 2024 · ISME Communications

DOI: https://doi.org/10.1093/ismeco/ycae064 · OSTI ID:2367243

Goff, Jennifer L.; Lui, Lauren M.; Nielsen, Torben N.; Poole, Farris L.; Smith, Heidi J.; Walker, Kathleen F.; Hazen, Terry C.; Fields, Matthew W.; Arkin, Adam P.; Adams, Michael W. W.

Abstract Mobile genetic elements (MGEs) like plasmids, viruses, and transposable elements can provide fitness benefits to their hosts for survival in the presence of environmental stressors. Heavy metal resistance genes (HMRGs) are frequently observed on MGEs, suggesting that MGEs may be an important driver of adaptive evolution in environments contaminated with heavy metals. Here, we report the meta-mobilome of the heavy metal-contaminated regions of the Oak Ridge Reservation subsurface. This meta-mobilome was compared with one derived from samples collected from unimpacted regions of the Oak Ridge Reservation subsurface. We assembled 1615 unique circularized DNA elements that we propose to be MGEs. The circular elements from the highly contaminated subsurface were enriched in HMRG clusters relative to those from the nearby unimpacted regions. Additionally, we found that these HMRGs were associated with Gamma and Betaproteobacteria hosts in the contaminated subsurface and potentially facilitate the persistence and dominance of these taxa in this region. Finally, the HMRGs were associated with conjugative elements, suggesting their potential for future lateral transfer. We demonstrate how our understanding of MGE ecology, evolution, and function can be enhanced through the genomic context provided by completed MGE assemblies.

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Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2367243

Alternate ID(s):: OSTI ID: 2396880

Journal Information:: ISME Communications, Journal Name: ISME Communications Journal Issue: 1 Vol. 4; ISSN 2730-6151

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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