Regiospecific Cellulose Orientation and Anisotropic Mechanical Property in Plant Cell Walls

Lee, Jongcheol; Choi, Juseok; Feng, Luyi; Yu, Jingyi; Zheng, Yunzhen; Zhang, Qian; Lin, Yen-Ting; Sah, Saroj; Gu, Ying; Zhang, Sulin; Cosgrove, Daniel J.; Kim, Seong H.

doi:10.1021/acs.biomac.3c00538

Title: Regiospecific Cellulose Orientation and Anisotropic Mechanical Property in Plant Cell Walls

Journal Article · 12 September 2023 · Biomacromolecules

DOI: https://doi.org/10.1021/acs.biomac.3c00538 · OSTI ID:2349339

^[1];

^[2]; Feng, Luyi ^[2]; Yu, Jingyi ^[2]; Zheng, Yunzhen ^[2]; Zhang, Qian ^[2]; Lin, Yen-Ting ^[2]; Sah, Saroj ^[2]; Gu, Ying ^[2]; Zhang, Sulin ^[2];

^[2];

^[2]

Pennsylvania State University, University Park, PA (United States); Pennsylvania State University
Pennsylvania State University, University Park, PA (United States)

Cellulose microfibrils (CMFs) are a major load-bearing component in plant cell walls. Thus, their structures have been studied extensively with spectroscopic and microscopic characterization methods; but the findings from these two approaches were inconsistent, which hampers mechanistic understanding of cell wall mechanics. Here, we report the regiospecific assembly of CMFs in the periclinal wall of plant epidermal cells. Using sum frequency generation spectroscopic imaging, we found that CMFs are highly aligned in the cell edge region where two cells form a junction, whereas they are mostly isotropic on average throughout the wall thickness in the flat face region of the epidermal cell. This subcellular-level heterogeneity in CMF alignment provided insights into tissue-level anisotropy in tensile modulus of cell wall materials. Furthermore, this finding also has resolved a previous contradiction between the spectroscopic and microscopic imaging studies, which paves a foundation for better understanding of cell wall artichecture, especially structure-geometry relationships.

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Research Organization:: Pennsylvania State University, University Park, PA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001090

OSTI ID:: 2349339

Journal Information:: Biomacromolecules, Journal Name: Biomacromolecules Journal Issue: 11 Vol. 24; ISSN 1525-7797

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Sum frequency generation (SFG) spectroscopy
cellulose microfibrils
plant cell wall mechanics
finite element analysis (FEA)

Title: Regiospecific Cellulose Orientation and Anisotropic Mechanical Property in Plant Cell Walls

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