Stress-tolerant non-conventional microbes enable next-generation chemical biosynthesis

Thorwall, Sarah; Schwartz, Cory; Chartron, Justin W.; Wheeldon, Ian

doi:10.1038/s41589-019-0452-x

Title: Stress-tolerant non-conventional microbes enable next-generation chemical biosynthesis

Journal Article · 23 January 2020 · Nature Chemical Biology

DOI: https://doi.org/10.1038/s41589-019-0452-x · OSTI ID:2345778

Thorwall, Sarah ^[1]; Schwartz, Cory ^[2]; Chartron, Justin W. ^[2];

^[2]

Univ. of California, Riverside, CA (United States); University of California, Riverside
Univ. of California, Riverside, CA (United States)

Microbial chemical production is a rapidly growing industry, with much of the growth fueled by advances in synthetic biology. New approaches have enabled rapid strain engineering for the production of various compounds; however, translation to industry is often problematic because native phenotypes of model hosts prevent the design of new low-cost bioprocesses. Here, in this work, we argue for a new approach that leverages the native stress-tolerant phenotypes of non-conventional microbes that directly address design challenges from the outset. Growth at high temperature, high salt and solvent concentrations, and low pH can enable cost savings by reducing the energy required for product separation, bioreactor cooling, and maintaining sterile conditions. These phenotypes have the added benefit of allowing for the use of low-cost sugar and water resources. Non-conventional hosts are needed because these phenotypes are polygenic and thus far have proven difficult to recapitulate in the common hosts Escherichia coli and Saccharomyces cerevisiae.

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Research Organization:: Univ. of California, Riverside, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0019093

OSTI ID:: 2345778

Journal Information:: Nature Chemical Biology, Journal Name: Nature Chemical Biology Journal Issue: 2 Vol. 16; ISSN 1552-4450

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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