Biocomposite thermoplastic polyurethanes containing evolved bacterial spores as living fillers to facilitate polymer disintegration

Kim, Han Sol; Noh, Myung Hyun; White, Evan M.; Kandefer, Michael V.; Wright, Austin F.; Datta, Debika; Lim, Hyun Gyu; Smiggs, Ethan; Locklin, Jason J.; Rahman, Md Arifur; Feist, Adam M.; Pokorski, Jonathan K.

doi:10.1038/s41467-024-47132-8

Title: Biocomposite thermoplastic polyurethanes containing evolved bacterial spores as living fillers to facilitate polymer disintegration

Journal Article · 30 April 2024 · Nature Communications

DOI: https://doi.org/10.1038/s41467-024-47132-8 · OSTI ID:2342168

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^[3]; Wright, Austin F. ^[3];

^[1]; Lim, Hyun Gyu ^[1]; Smiggs, Ethan ^[1];

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University of California San Diego, La Jolla, CA (United States)
University of California San Diego, La Jolla, CA (United States); Korea Research Institute of Chemical Technology (KRICT), Ulsan (Korea, Republic of)
University of Georgia, Athens, GA (United States)
BASF Corporation, Wyandotte, MI (United States)
University of California San Diego, La Jolla, CA (United States); Technical University of Denmark, Lyngby (Denmark)

The field of hybrid engineered living materials seeks to pair living organisms with synthetic materials to generate biocomposite materials with augmented function since living systems can provide highly-programmable and complex behavior. Engineered living materials have typically been fabricated using techniques in benign aqueous environments, limiting their application. In this work, biocomposite fabrication is demonstrated in which spores from polymer-degrading bacteria are incorporated into a thermoplastic polyurethane using high-temperature melt extrusion. Bacteria are engineered using adaptive laboratory evolution to improve their heat tolerance to ensure nearly complete cell survivability during manufacturing at 135 °C. Furthermore, the overall tensile properties of spore-filled thermoplastic polyurethanes are substantially improved, resulting in a significant improvement in toughness. The biocomposites facilitate disintegration in compost in the absence of a microbe-rich environment. Finally, embedded spores demonstrate a rationally programmed function, expressing green fluorescent protein. This research provides a scalable method to fabricate advanced biocomposite materials in industrially-compatible processes.

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Research Organization:: University of California San Diego, La Jolla, CA (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Energy Efficiency Office. Advanced Materials & Manufacturing Technologies Office (AMMTO); USDOE

Grant/Contract Number:: EE0009296; AC02-05CH11231

OSTI ID:: 2342168

Alternate ID(s):: OSTI ID: 2341937

Journal Information:: Nature Communications, Vol. 15, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Biocomposite thermoplastic polyurethanes containing evolved bacterial spores as living fillers to facilitate polymer disintegration

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