DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Chapter 8: Searching for Life Beyond Earth

Journal Article · · Astrobiology
 [1];  [2];  [3];  [4];  [5];  [6];  [7];  [7];  [8];  [9]
  1. NASA Goddard Space Flight Center (GSFC), Greenbelt, MD (United States); Univ. of Maryland, Baltimore, MD (United States); Georgetown University, Washington, DC (United States)
  2. Santa Fe Institute, Santa Fe, NM (United States); Blue Marble Space Institute of Science, Seattle, WA (United States)
  3. Northern Arizona University, Flagstaff, AZ (United States)
  4. University of Colorado, Boulder, CO (United States)
  5. The BioRobotics Institute, Pisa (Italy)
  6. NASA Ames Research Center (ARC), Moffett Field, Mountain View, CA (United States)
  7. Georgia Institute of Technology, Atlanta, GA (United States)
  8. Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
  9. NASA Goddard Space Flight Center (GSFC), Greenbelt, MD (United States); Northern Arizona University, Flagstaff, AZ (United States)

The search for life beyond Earth necessitates a rigorous and comprehensive examination of biosignatures, the types of observable imprints that life produces. These imprints and our ability to detect them with advanced instrumentation hold the key to our understanding of the presence and abundance of life in the universe. Biosignatures are the chemical or physical features associated with past or present life and may include the distribution of elements and molecules, alone or in combination, as well as changes in structural components or physical processes that would be distinct from an abiotic background. The scientific and technical strategies used to search for life on other planets include those that can be conducted in situ to planetary bodies and those that could be observed remotely. Here this chapter discusses numerous strategies that can be employed to look for biosignatures directly on other planetary bodies using robotic exploration including those that have been deployed to other planetary bodies, are currently being developed for flight, or will become a critical technology on future missions. Search strategies for remote observations using current and planned ground-based and space-based telescopes are also described. Evidence from spectral absorption, emission, or transmission features can be used to search for remote biosignatures and technosignatures. Improving our understanding of biosignatures, their production, transformation, and preservation on Earth can enhance our search efforts to detect life on other planets.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA); National Aeronautics and Space Administration (NASA); National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Laboratory Directed Research and Development (LDRD) Program
Grant/Contract Number:
AC52-07NA27344
OSTI ID:
2341952
Report Number(s):
LLNL-JRNL-825389; 1039424
Journal Information:
Astrobiology, Journal Name: Astrobiology Journal Issue: S1 Vol. 24; ISSN 1531-1074
Publisher:
Mary Ann Liebert, Inc.Copyright Statement
Country of Publication:
United States
Language:
English

References (158)

The Origin of Life-Out of the Blue journal October 2015
Ancient biomolecules: Their origins, fossilization, and role in revealing the history of life: Prospects & Overviews journal March 2014
Electrochemistry for Life Detection on Ocean Worlds journal January 2020
Morphological biosignatures from relict fossilised sedimentary geological specimens: a Raman spectroscopic study journal July 2007
Remote sensing of aquatic coastal ecosystem processes book January 2006
The Diagenesis of Proteins and Amino Acids in Fossil Shells book January 1993
Stable Isotope Probing book January 2019
Surface and Temporal Biosignatures book November 2018
Biosignatures for Astrobiology book October 2018
Geobiology book January 2010
Geological Sources of Methane book January 2000
Limestones are Biological Sediments book January 2004
The structure of scytonemin, an ultraviolet sunscreen pigment from the sheaths of cyanobacteria journal September 1993
Estimating microbial mat biomass in the McMurdo Dry Valleys, Antarctica using satellite imagery and ground surveys journal September 2020
Metalliferous Biosignatures for Deep Subsurface Microbial Activity journal September 2015
Circular spectropolarimetric sensing of higher plant and algal chloroplast structural variations journal August 2018
Molecular Biosignatures journal October 2007
The Sample Analysis at Mars Investigation and Instrument Suite journal April 2012
The Thirty Meter Telescope (TMT): An International Observatory journal June 2013
Laser-induced breakdown spectroscopy (LIBS): a novel technology for identifying microbes causing infectious diseases journal October 2018
Nuclear waste spectrum as evidence of technological extraterrestrial civilizations journal April 1980
Red and photographic infrared linear combinations for monitoring vegetation journal May 1979
Formation and diagenesis of macromolecular organic sulfur in Peru margin sediments journal December 1994
Stable carbon isotope fractionation in the search for life on early mars journal January 1989
Remote Sensing of Homochirality: A Proxy for the Detection of Extraterrestrial Life book June 2018
Breakthrough Listen – A new search for life in the universe journal October 2017
The importance of non-photosynthetic pigments and cinnamic acid derivatives in photoprotection journal April 2005
Biomarkers (molecular fossils) as geochemical indicators of life journal January 2004
Stable isotope probing with 18O-water to investigate microbial growth and death in environmental samples journal October 2016
Refractory and semi-volatile organics at the surface of comet 67P/Churyumov-Gerasimenko: Insights from the VIRTIS/Rosetta imaging spectrometer journal July 2016
Application of chiral derivatizing agents in the high-performance liquid chromatographic separation of amino acid enantiomers: A review journal May 2008
Fluorescence in situ hybridization (FISH): History, limitations and what to expect from micro-scale FISH? journal November 2018
Instrument development to search for biomarkers on mars: Terrestrial acidophile, iron-powered chemolithoautotrophic communities as model systems journal June 2005
The specific molecular identification of life experiment (SMILE) journal July 2005
A new extraction technique for in situ analyses of amino and carboxylic acids on Mars by gas chromatography mass spectrometry journal December 2006
Development status of the life marker chip instrument for ExoMars journal November 2012
Searching for biomolecules on Mars: Considerations for operation of a life marker chip instrument journal September 2013
Absorption spectrum of phytoplankton pigments derived from hyperspectral remote-sensing reflectance journal February 2004
Application of hyperspectral remote sensing to cyanobacterial blooms in inland waters journal September 2015
Expanding the solar spectrum used by photosynthesis journal August 2011
Detection of martian amino acids by chemical derivatization coupled to gas chromatography: In situ and laboratory analysis journal January 2001
Organic preservation of non-mineralizing organisms and the taphonomy of the Burgess Shale journal January 1990
High-resolution compositional remote sensing of the Transantarctic Mountains: application to the WorldView-2 dataset journal May 2015
Remote characterization of photosynthetic communities in the Fryxell basin of Taylor Valley, Antarctica journal March 2020
The evolution of life in the Universe: are we alone? journal August 2006
Enhanced Resolution of Chiral Amino Acids with Capillary Electrophoresis for Biosignature Detection in Extraterrestrial Samples journal December 2016
Lipid Synthesis Under Hydrothermal Conditions by Fischer- Tropsch-Type Reactions journal March 1999
The Gas Chromatograph Mass Spectrometer for the Huygens Probe journal July 2002
Isotopic Fractionation by Gravitational Escape journal April 2003
Origin of oxygen species in Titan's atmosphere journal January 2008
Seasonal Variations in Atmospheric Composition as Measured in Gale Crater, Mars journal November 2019
Ocean color chlorophyll algorithms for SeaWiFS journal October 1998
The search for organic substances and inorganic volatile compounds in the surface of Mars journal September 1977
Signs of Life: Criterion-System of Exobiology journal July 1965
A Physical Basis for Life Detection Experiments journal August 1965
2-Methylhopanoids as biomarkers for cyanobacterial oxygenic photosynthesis journal August 1999
Evidence from carbon isotope measurements for diverse origins of sedimentary hydrocarbons journal January 1990
A planetary system around the millisecond pulsar PSR1257 + 12 journal January 1992
A search for life on Earth from the Galileo spacecraft journal October 1993
Biosignatures as revealed by spectropolarimetry of Earthshine journal February 2012
Phosphine gas in the cloud decks of Venus journal September 2020
Reassessing evidence of life in 3,700-million-year-old rocks of Greenland journal October 2018
Call for a framework for reporting evidence for life beyond Earth journal October 2021
Radiation Tolerance of Nanopore Sequencing Technology for Life Detection on Mars and Europa journal March 2019
The thermodynamic properties of isotopic substances journal January 1947
Purification and properties of chlorophyll c from Sargassum flavicans journal February 1963
Re-analysis of the 267 GHz ALMA observations of Venus journal December 2020
Miniaturized Scanning Electron Microscope for In Situ Planetary Studies conference March 2010
Project Ozma journal April 1961
A combined electrochemical and optical trapping platform for measuring single cell respiration rates at electrode interfaces journal June 2015
Detection of single analyte and environmental samples with silicon nitride nanopores: Antarctic dirt particulates and DNA in artificial seawater journal March 2020
Detection of circular polarization in light scattered from photosynthetic microbes journal April 2009
Surface biosignatures of exo-Earths: Remote detection of extraterrestrial life journal March 2015
A STATISTICAL RECONSTRUCTION OF THE PLANET POPULATION AROUND KEPLER SOLAR-TYPE STARS journal January 2015
Terrestrial exoplanets: diversity, habitability and characterization journal July 2008
A Shiny New Method for SETI: Specular Reflections from Interplanetary Artifacts journal June 2019
Mineralogical Biosignatures and the Search for Life on Mars journal December 2001
Mass-Independent Fractionation of Sulfur Isotopes in Archean Sediments: Strong Evidence for an Anoxic Archean Atmosphere journal March 2002
Biosignatures of Early Earths journal September 2003
Morphological Biosignatures and the Search for Life on Mars journal June 2003
Vegetation's Red Edge: A Possible Spectroscopic Biosignature of Extraterrestrial Plants journal June 2005
Biomarker and Stable Isotope Characterization of Coastal Pond-Derived Organic Matter, McMurdo Dry Valleys, Antarctica journal August 2007
Identification of Morphological Biosignatures in Martian Analogue Field Specimens Using In Situ Planetary Instrumentation journal February 2008
Spectral Signatures of Photosynthesis. I. Review of Earth Organisms journal February 2007
Spectral Signatures of Photosynthesis. II. Coevolution with Other Stars And The Atmosphere on Extrasolar Worlds journal February 2007
Fluorescence Microscopy as a Tool for In Situ Life Detection journal August 2008
Science Priorities for Mars Sample Return journal June 2008
The NASA Astrobiology Roadmap journal August 2008
Laser-Induced Fluorescence Emission (L.I.F.E.): In Situ Nondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes journal September 2009
The Far Future of Exoplanet Direct Characterization journal January 2010
Distribution and Stable Isotopic Composition of Amino Acids from Fungal Peptaibiotics: Assessing the Potential for Meteoritic Contamination journal March 2011
Properties of an Earth-Like Planet Orbiting a Sun-Like Star: Earth Observed by the EPOXI Mission journal November 2011
Earth as an Extrasolar Planet: Earth Model Validation Using EPOXI Earth Observations journal June 2011
A Microbial Oasis in the Hypersaline Atacama Subsurface Discovered by a Life Detector Chip: Implications for the Search for Life on Mars journal December 2011
Nonphotosynthetic Pigments as Potential Biosignatures journal May 2015
Extreme Water Loss and Abiotic O 2 Buildup on Planets Throughout the Habitable Zones of M Dwarfs journal February 2015
Ultrastructural Heterogeneity of Carbonaceous Material in Ancient Cherts: Investigating Biosignature Origin and Preservation journal October 2015
On Detecting Biospheres from Chemical Thermodynamic Disequilibrium in Planetary Atmospheres journal January 2016
Microbial Morphology and Motility as Biosignatures for Outer Planet Missions journal October 2016
Toward a List of Molecules as Potential Biosignature Gases for the Search for Life on Exoplanets and Applications to Terrestrial Biochemistry journal June 2016
A New Empirical Constraint on the Prevalence of Technological Species in the Universe journal May 2016
The Pale Orange Dot: The Spectrum and Habitability of Hazy Archean Earth journal November 2016
Modeling pN2 through Geological Time: Implications for Planetary Climates and Atmospheric Biosignatures journal December 2016
Reflections on O2as a Biosignature in Exoplanetary Atmospheres journal October 2017
Biosignature Preservation and Detection in Mars Analog Environments journal April 2017
Exoplanet Biosignatures: Understanding Oxygen as a Biosignature in the Context of Its Environment journal June 2018
Exoplanet Biosignatures: A Review of Remotely Detectable Signs of Life journal June 2018
Exoplanet Biosignatures: A Framework for Their Assessment journal June 2018
Exoplanet Biosignatures: Future Directions journal June 2018
The Ladder of Life Detection journal November 2018
Phosphine as a Biosignature Gas in Exoplanet Atmospheres journal February 2020
Paleo-Rock-Hosted Life on Earth and the Search on Mars: A Review and Strategy for Exploration journal October 2019
Spectropolarimetry of Primitive Phototrophs as Global Surface Biosignatures journal February 2021
When is Life a Viable Hypothesis? The Case of Venusian Phosphine journal March 2021
Accounting for multiplicity in calculating eta Earth journal May 2019
The statistical reliability of 267-GHz JCMT observations of Venus: no significant evidence for phosphine absorption journal November 2020
Steroids, triterpenoids and molecular oxygen
  • Summons, Roger E.; Bradley, Alexander S.; Jahnke, Linda L.
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 361, Issue 1470 https://doi.org/10.1098/rstb.2006.1837
journal May 2006
Mars Sample Return as a campaign conference March 2011
Organics Analyzer for Sampling Icy Surfaces: A liquid chromatograph-mass spectrometer for future in situ small body missions conference March 2013
Towards in situ sequencing for life detection conference March 2017
Growth of saprotrophic fungi and bacteria in soil: Growth of saprotrophic fungi and bacteria in soil journal April 2011
Transiting Exoplanet Survey Satellite journal October 2014
The Giant Magellan Telescope (GMT) conference June 2006
Disequilibrium biosignatures over Earth history and implications for detecting exoplanet life journal January 2018
Gas Chromatographic Column for the Viking 1975 Molecular Analysis Experiment journal July 1975
Archean Molecular Fossils and the Early Rise of Eukaryotes journal August 1999
Microbial Biofilm Voltammetry: Direct Electrochemical Characterization of Catalytic Electrode-Attached Biofilms journal October 2008
Microbial uptake of radiolabeled substrates: estimates of growth rates from time course measurements journal January 1984
Experimental evidence that clay inhibits bacterial decomposers: Implications for preservation of organic fossils journal August 2016
Stromatolites and MISS—Differences between relatives journal September 2013
Geobiology: Evidence for early life on Earth and the search for life on other planets journal November 2009
Ichnotaxonomy of microbial trace fossils in volcanic glass journal January 2009
The Search for Extraterrestrial Intelligence (SETI) journal September 2001
Astrobiology: Exploring the Origins, Evolution, and Distribution of Life in the Universe journal November 1999
HPLC-DAD-ESI/MS Identification of Light Harvesting and Light Screening Pigments in the Lake Sediments at Edmonson Point journal January 2013
The large-scale organization of chemical reaction networks in astrophysics journal October 2004
A Submersible, Off-Axis Holographic Microscope for Detection of Microbial Motility and Morphology in Aqueous and Icy Environments journal January 2016
PhotochemCAD 2. A Refined Program with Accompanying Spectral Database for Photochemical Calculations journal January 2004
ChemCam: Chemostratigraphy by the First Mars Microprobe journal February 2015
On the nature and significance of rarity in mineralogy journal June 2016
Building the Biomarker Tree of Life journal January 2005
The evolution of the sulfur cycle journal November 1999
Biofilms: Microbial Life on Surfaces journal September 2002
The Grand Challenges of Exoplanets journal April 2020
Planetary Mass Spectrometry for Agnostic Life Detection in the Solar System journal October 2021
The Detection of Long-Chain Bio-Markers Using Atomic Force Microscopy journal March 2019
A Statistical Estimation of the Occurrence of Extraterrestrial Intelligence in the Milky Way Galaxy journal January 2021
Defining Lyfe in the Universe: From Three Privileged Functions to Four Pillars journal April 2020
The Grayness of the Origin of Life journal May 2021
The Detectability and Characterization of the TRAPPIST-1 Exoplanet Atmospheres with JWST journal June 2019
Occurrence Rates of Planets Orbiting FGK Stars: Combining Kepler DR25, Gaia DR2, and Bayesian Inference journal August 2019
The Breakthrough Listen Search for Intelligent Life: Observations of 1327 Nearby Stars Over 1.10–3.45 GHz journal February 2020
The Breakthrough Listen Search for Intelligent Life: A 3.95–8.00 GHz Search for Radio Technosignatures in the Restricted Earth Transit Zone journal June 2020
The Occurrence of Rocky Habitable-zone Planets around Solar-like Stars from Kepler Data journal December 2020
When is Chemical Disequilibrium in Earth-like Planetary Atmospheres a Biosignature versus an Anti-biosignature? Disequilibria from Dead to Living Worlds journal April 2020
The Kepler Peas in a Pod Pattern is Astrophysical journal April 2020
Claimed Detection of PH3 in the Clouds of Venus Is Consistent with Mesospheric SO2 journal February 2021
Characterizing a cyanobacterial bloom in Western Lake Erie using satellite imagery and meteorological data journal August 2010