Engineering tertiary chirality in helical biopolymers

Janowski, Jordan; Pham, Van A. B.; Vecchioni, Simon; Woloszyn, Karol; Lu, Brandon; Zou, Yijia; Erkalo, Betel; Perren, Lara; Rueb, Joe; Madnick, Jesse; Mao, Chengde; Saito, Masahico; Ohayon, Yoel P.; Jonoska, Nataša; Sha, Ruojie

doi:10.1073/pnas.2321992121

Title: Engineering tertiary chirality in helical biopolymers

Journal Article · 28 April 2024 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.2321992121 · OSTI ID:2341646

Janowski, Jordan ^[1];

^[2];

^[1]; Woloszyn, Karol ^[1];

^[1]; Zou, Yijia ^[1]; Erkalo, Betel ^[1];

^[1]; Rueb, Joe ^[1];

^[3];

^[4];

^[2];

^[1]; Jonoska, Nataša ^[2];

^[1]

Department of Chemistry, New York University, New York, NY 10003
Department of Mathematics and Statistics, University of South Florida, Tampa, FL 33620
Department of Mathematics, University of Oregon, Eugene, OR 97403
Department of Chemistry, Purdue University, West Lafayette, IN 47907

Tertiary chirality describes the handedness of supramolecular assemblies and relies not only on the primary and secondary structures of the building blocks but also on topological driving forces that have been sparsely characterized. Helical biopolymers, especially DNA, have been extensively investigated as they possess intrinsic chirality that determines the optical, mechanical, and physical properties of the ensuing material. Here, we employ the DNA tensegrity triangle as a model system to locate the tipping points in chirality inversion at the tertiary level by X-ray diffraction. We engineer tensegrity triangle crystals with incremental rotational steps between immobile junctions from 3 to 28 base pairs (bp). We construct a mathematical model that accurately predicts and explains the molecular configurations in both this work and previous studies. Our design framework is extendable to other supramolecular assemblies of helical biopolymers and can be used in the design of chiral nanomaterials, optically active molecules, and mesoporous frameworks, all of which are of interest to physical, biological, and chemical nanoscience.

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Research Organization:: New York Univ. (NYU), NY (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: SC0007991

OSTI ID:: 2341646

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 19 Vol. 121; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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