Transcriptomics-Based Points of Departure for Daphnia magna Exposed to 18 Per- and Polyfluoroalkyl Substances
[1];
[3];
[1]
- US Environmental Protection Agency (EPA), Duluth, MN (United States)
- US Environmental Protection Agency (EPA), Duluth, MN (United States); Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States); US Environmental Protection Agency (EPA), Duluth, MN (United States)
- US Environmental Protection Agency (EPA), Oak Ridge, NC (United States)
- Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States); US Environmental Protection Agency (EPA), Oak Ridge, NC (United States)
Here, per- and polyfluoroalkyl substances (PFAS) represent a large group of contaminants of concern based on their widespread use, environmental persistence, and potential toxicity. Many traditional models for estimating toxicity, bioaccumulation, and other toxicological properties are not well suited for PFAS. Consequently, there is a need to generate hazard information for PFAS in an efficient and cost-effective manner. In the present study, Daphnia magna were exposed to multiple concentrations of 22 different PFAS for 24 h, in 96-well plate format. Following exposure, whole body RNA was extracted and extracts, each representing five exposed individuals, were subjected to RNA sequencing. Following analytical measurements to verify PFAS exposure concentrations, and quality control on processed cDNA libraries for sequencing, concentration-response modeling was applied to the data sets for 18 of the tested compounds, and the concentration at which a concerted molecular response occurred (transcriptomic point of departure; tPOD) was calculated. The tPODs, based on measured concentrations of PFAS, generally ranged from 0.03-0.58 µM (9.9-350 µg/L; interquartile range). In most cases, these concentrations were two orders of magnitude lower than similarly calculated tPODs for human cell lines exposed to PFAS. They were also lower than apical effect concentrations reported for seven PFAS for which some crustacean or invertebrate toxicity data were available, although there were a few exceptions. Despite being lower than most other available hazard benchmarks, Daphnia magna tPODs were, on average, four orders of magnitude greater than the maximum aqueous concentrations of PFAS measured in Great Lakes tributaries. Overall, this high throughput transcriptomics assay with Daphnia magna holds promise as a component of a tiered hazard evaluation strategy employing new approach methodologies. Environ Toxicol Chem 2024;00:1-16. © 2024 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Organization:
- USDOE National Nuclear Security Administration (NNSA)
- Grant/Contract Number:
- 89233218CNA000001
- OSTI ID:
- 2340889
- Report Number(s):
- LA-UR--23-29437; {"","Journal ID: ISSN 0730-7268"}
- Journal Information:
- Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry; ISSN 0730-7268
- Publisher:
- SETACCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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