Recent mechanistic developments for cytochrome c nitrite reductase, the key enzyme in the dissimilatory nitrate reduction to ammonium pathway

Hird, Krystina; Campeciño, Julius O.; Lehnert, Nicolai; Hegg, Eric L.

doi:10.1016/j.jinorgbio.2024.112542

Title: Recent mechanistic developments for cytochrome c nitrite reductase, the key enzyme in the dissimilatory nitrate reduction to ammonium pathway

Journal Article · 23 March 2024 · Journal of Inorganic Biochemistry

DOI: https://doi.org/10.1016/j.jinorgbio.2024.112542 · OSTI ID:2339604

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Michigan State University, East Lansing, MI (United States); Michigan State University
Michigan State University, East Lansing, MI (United States)
University of Michigan, Ann Arbor, MI (United States)

Cytochrome c nitrite reductase, NrfA, is a soluble, periplasmic pentaheme cytochrome responsible for the reduction of nitrite to ammonium in the Dissimilatory Nitrate Reduction to Ammonium (DNRA) pathway, a vital reaction in the global nitrogen cycle. NrfA catalyzes this six-electron and eight-proton reduction of nitrite at a single active site with the help of its quinol oxidase partners. In this study, we summarize the latest progress in elucidating the reaction mechanism of ammonia production, including new findings about the active site architecture of NrfA, as well as recent results that elucidate electron transfer and storage in the pentaheme scaffold of this enzyme.

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Research Organization:: Michigan State University, East Lansing, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation Graduate Research Fellowship Program

Grant/Contract Number:: SC0017952; SC0018173

OSTI ID:: 2339604

Alternate ID(s):: OSTI ID: 2337747

Journal Information:: Journal of Inorganic Biochemistry, Journal Name: Journal of Inorganic Biochemistry Vol. 256; ISSN 0162-0134

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
NrfA
Cytochrome c nitrite reductase
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Crystal structure

Title: Recent mechanistic developments for cytochrome c nitrite reductase, the key enzyme in the dissimilatory nitrate reduction to ammonium pathway

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