Genomic Insights into Moderately Thermophilic Methanotrophs of the Genus Methylocaldum

Delherbe, Nathalie A.; Pearce, David; But, Sergey Y.; Murrell, J. Colin; Khmelenina, Valentina N.; Kalyuzhnaya, Marina G.

doi:10.3390/microorganisms12030469

Title: Genomic Insights into Moderately Thermophilic Methanotrophs of the Genus Methylocaldum

Journal Article · 25 February 2024 · Microorganisms

DOI: https://doi.org/10.3390/microorganisms12030469 · OSTI ID:2338243

^[1]; Pearce, David ^[2];

^[3]; Murrell, J. Colin ^[2]; Khmelenina, Valentina N. ^[3];

^[4]

San Diego State University, CA (United States); San Diego State University
University of East Anglia, Norwich (United Kingdom)
Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino (Russia)
San Diego State University, CA (United States)

Considering the increasing interest in understanding the biotic component of methane removal from our atmosphere, it becomes essential to study the physiological characteristics and genomic potential of methanotroph isolates, especially their traits allowing them to adapt to elevated growth temperatures. The genetic signatures of Methylocaldum species have been detected in many terrestrial and aquatic ecosystems. A small set of representatives of this genus has been isolated and maintained in culture. The genus is commonly described as moderately thermophilic, with the growth optimum reaching 50 °C for some strains. Here, we present a comparative analysis of genomes of three Methylocaldum strains—two terrestrial M. szegediense strains (O-12 and Norfolk) and one marine strain, Methylocaldum marinum (S8). The examination of the core genome inventory of this genus uncovers significant redundancy in primary metabolic pathways, including the machinery for methane oxidation (numerous copies of pmo genes) and methanol oxidation (duplications of mxaF, xoxF1-5 genes), three pathways for one-carbon (C1) assimilation, and two methods of carbon storage (glycogen and polyhydroxyalkanoates). We also investigate the genetics of melanin production pathways as a key feature of the genus.

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Research Organization:: San Diego State University, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0024289; AC02-05CH11231

OSTI ID:: 2338243

Journal Information:: Microorganisms, Journal Name: Microorganisms Journal Issue: 3 Vol. 12; ISSN 2076-2607

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
thermophilic methanotrophic bacteria
Methylocaldum
methane monooxygenase
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methanol dehydrogenase
extremophiles

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