Mo than meets the eye: genomic insights into molybdoenzyme diversity of Seleniivibrio woodruffii strain S4T

Louie, Tiffany S.; Kumar, Anil; Bini, Elisabetta; Häggblom, Max M.

doi:10.1093/lambio/ovae038

Title: Mo than meets the eye: genomic insights into molybdoenzyme diversity of Seleniivibrio woodruffii strain S4T

Journal Article · 03 April 2024 · Letters in Applied Microbiology

DOI: https://doi.org/10.1093/lambio/ovae038 · OSTI ID:2336854

Louie, Tiffany S.; Kumar, Anil; Bini, Elisabetta;

Abstract Seleniivibrio woodruffii strain S4T is an obligate anaerobe belonging to the phylum Deferribacterota. It was isolated for its ability to respire selenate and was also found to respire arsenate. The high-quality draft genome of this bacterium is 2.9 Mbp, has a G+C content of 48%, 2762 predicted genes of which 2709 are protein-coding, and 53 RNA genes. An analysis of the genome focusing on the genes encoding for molybdenum-containing enzymes (molybdoenzymes) uncovered a remarkable number of genes encoding for members of the dimethylsulfoxide reductase family of proteins (DMSOR), including putative reductases for selenate and arsenate respiration, as well as genes for nitrogen fixation. Respiratory molybdoenzymes catalyze redox reactions that transfer electrons to a variety of substrates that can act as terminal electron acceptors for energy generation. Seleniivibrio woodruffii strain S4T also has essential genes for molybdate transporters and the biosynthesis of the molybdopterin guanine dinucleotide cofactors characteristic of the active centers of DMSORs. Phylogenetic analysis revealed candidate respiratory DMSORs spanning nine subfamilies encoded within the genome. Our analysis revealed the untapped potential of this interesting microorganism and expanded our knowledge of molybdoenzyme co-occurrence.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2336854

Journal Information:: Letters in Applied Microbiology, Journal Name: Letters in Applied Microbiology Journal Issue: 4 Vol. 77; ISSN 1472-765X

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Country unknown/Code not available

Language:: English

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