Utilization of lasso peptides for biodegradation of polycyclic aromatic hydrocarbons
Abstract Many microbial genes involved in degrading recalcitrant environmental contaminants such as polycyclic aromatic hydrocarbons (PAHs) have been identified and characterized. However, all molecular mechanisms required for PAH utilization have not yet been elucidated. In this work, we demonstrate the proposed involvement of lasso peptides in the utilization of the PAH phenanthrene in Sphingomonas BPH. Transpositional mutagenesis of Sphingomonas BPH with the miniTn5 transposon yielded 3 phenanthrene utilization deficient mutants, #257, #1778, and #1782. In mutant #1782, Tn5 had inserted into the large subunit of the naph/bph dioxygenase gene. In mutant #1778, Tn5 had inserted into the B2 protease gene of a lasso peptide cluster. This finding is the first report on the role of lasso peptides in PAH utilization. Our studies also demonstrate that interruption of the lasso peptide cluster resulted in a significant increase in the amount of biosurfactant produced in the presence of glucose when compared to the wild‐type strain. Collectively, these results suggest that the mechanisms Sphingomonas BPH utilizes to degrade phenanthrene are far more complex than previously understood and that the #1778 mutant may be a good candidate for bioremediation when glucose is applied as an amendment due to its higher biosurfactant production.
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- 89303321CEM000080
- OSTI ID:
- 2336622
- Alternate ID(s):
- OSTI ID: 2336623; OSTI ID: 2471783
- Journal Information:
- Environmental Microbiology Reports, Journal Name: Environmental Microbiology Reports Journal Issue: 2 Vol. 16; ISSN 1758-2229
- Publisher:
- Wiley-BlackwellCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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