Structural analysis of wild-type and Val120Thr mutant Candida boidinii formate dehydrogenase by X-ray crystallography

Gul, Mehmet; Yuksel, Busra; Bulut, Huri; DeMirci, Hasan

doi:10.1107/s2059798323008070

Title: Structural analysis of wild-type and Val120Thr mutant Candida boidinii formate dehydrogenase by X-ray crystallography

Journal Article · 19 October 2023 · Acta Crystallographica. Section D. Structural Biology

DOI: https://doi.org/10.1107/s2059798323008070 · OSTI ID:2327111

Gul, Mehmet ^[1]; Yuksel, Busra ^[2]; Bulut, Huri ^[3]; DeMirci, Hasan ^[4]

Koc Univ., Istanbul (Turkey); SLAC
Koc Univ., Istanbul (Turkey); Max Planck Institute of Biophysics, Frankfurt (Germany)
Istinye Univ., Istanbul (Turkey)
Koc Univ., Istanbul (Turkey); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Photon Ultrafast Laser Science and Engineering Institute (PULSE)

Candida boidinii NAD⁺ -dependent formate dehydrogenase (CbFDH) has gained significant attention for its potential application in the production of biofuels and various industrial chemicals from inorganic carbon dioxide. The present study reports the atomic X-ray crystal structures of wild-type CbFDH at cryogenic and ambient temperatures, as well as that of the Val120Thr mutant at cryogenic temperature, determined at the Turkish Light Source ‘Turkish DeLight’. The structures reveal new hydrogen bonds between Thr120 and water molecules in the active site of the mutant CbFDH, suggesting increased stability of the active site and more efficient electron transfer during the reaction. Further experimental data is needed to test these hypotheses. Collectively, these findings provide invaluable insights into future protein-engineering efforts that could potentially enhance the efficiency and effectiveness of CbFDH.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation (NSF)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 2327111

Journal Information:: Acta Crystallographica. Section D. Structural Biology, Journal Name: Acta Crystallographica. Section D. Structural Biology Journal Issue: 11 Vol. 79; ISSN 2059-7983

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
formate dehydrogenases
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protein engineering
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Turkish Light Source
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Title: Structural analysis of wild-type and Val120Thr mutant Candida boidinii formate dehydrogenase by X-ray crystallography

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