Scale-up of microbial lipid and bioethanol production from oilcane

Deshavath, Narendra Naik; Woodruff, William; Eller, Fred; Susanto, Vionna; Yang, Cindy; Rao, Christopher V.; Singh, Vijay

doi:10.1016/j.biortech.2024.130594

Title: Scale-up of microbial lipid and bioethanol production from oilcane

Journal Article · 15 March 2024 · Bioresource Technology

DOI: https://doi.org/10.1016/j.biortech.2024.130594 · OSTI ID:2325206

Deshavath, Narendra Naik ^[1];

^[2]; Eller, Fred ^[3]; Susanto, Vionna ^[2]; Yang, Cindy ^[2]; Rao, Christopher V. ^[2];

^[1]

University of Illinois Urbana Champaign, IL (United States); Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois at Urbana-Champaign, IL (United States)
United States Department of Agriculture, Agricultural Research Service, Peoria, IL (United States)

Microbial oils are a sustainable biomass-derived substitute for liquid fuels and vegetable oils. Oilcane, an engineered sugarcane with superior feedstock characteristics for biodiesel production, is a promising candidate for bioconversion. This study describes the processing of oilcane stems into juice and hydrothermally pretreated lignocellulosic hydrolysate and their valorization to ethanol and microbial oil using Saccharomyces cerevisiae and engineered Rhodosporidium toruloides strains, respectively. A bioethanol titer of 106 g/L was obtained from S. cerevisiae grown on oilcane juice in a 3 L fermenter, and a lipid titer of 8.8 g/L was obtained from R. toruloides grown on oilcane hydrolysate in a 75 L fermenter. Oil was extracted from the R. toruloides cells using supercritical CO₂, and the observed fatty acid profile was consistent with previous studies on this strain. These results demonstrate the feasibility of pilot-scale lipid production from oilcane hydrolysate as part of an integrated bioconversion strategy.

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Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science (BSS); USDOE

Grant/Contract Number:: SC0018420

OSTI ID:: 2325206

Alternate ID(s):: OSTI ID: 2377408

Journal Information:: Bioresource Technology, Vol. 399; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Scale-up of microbial lipid and bioethanol production from oilcane

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