Formation of a constructed microbial community in a nutrient-rich environment indicates bacterial interspecific competition

Wang, Jia; Appidi, Manasa R.; Burdick, Leah H.; Abraham, Paul E.; Hettich, Robert L.; Pelletier, Dale A.; Doktycz, Mitchel J.

doi:10.1128/msystems.00006-24

Title: Formation of a constructed microbial community in a nutrient-rich environment indicates bacterial interspecific competition

Journal Article · 12 March 2024 · mSystems

DOI: https://doi.org/10.1128/msystems.00006-24 · OSTI ID:2323356

^[1]; Appidi, Manasa R. ^[2]; Burdick, Leah H. ^[1];

^[1];

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Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

Understanding the organizational principles of microbial communities is essential for interpreting ecosystem stability. Previous studies have investigated the formation of bacterial communities under nutrient-poor conditions or obligate relationships to observe cooperative interactions among different species. How microorganisms form stabilized communities in nutrient-rich environments, without obligate metabolic interdependency for growth, is still not fully disclosed. In this study, three bacterial strains isolated from the Populus deltoides rhizosphere were co-cultured in complex medium, and their growth behavior was tracked. These strains co-exist in mixed culture over serial transfer for multiple growth-dilution cycles. Competition is proposed as an emergent interaction relationship among the three bacteria based on their significantly decreased growth levels. The effects of different initial inoculum ratios, up to three orders of magnitude, on community structure were investigated, and the final compositions of the mixed communities with various starting composition indicate that community structure is not dependent on the initial inoculum ratio. Furthermore, the competitive relationships within the community were not altered by different initial inoculum ratios. The community structure was simulated by generalized Lotka-Volterra and dynamic flux balance analysis to provide mechanistic predictions into emergence of community structure under a nutrient-rich environment. Metaproteomic analyses provide support for the metabolite exchanges predicted by computational modeling and for highly altered physiologies when microbes are grown in co-culture. These findings broaden our understanding of bacterial community dynamics and metabolic diversity in higher-order interactions and could be significant in the management of rhizospheric bacterial communities.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 2323356

Alternate ID(s):: OSTI ID: 2406809

Journal Information:: mSystems, Vol. 9, Issue 4; ISSN 2379-5077

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
microbial community
rhizospheric bacteria
initial inoculum ratio
dynamic flux balance analysis
metabolite exchange
metaproteomics

Title: Formation of a constructed microbial community in a nutrient-rich environment indicates bacterial interspecific competition

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