Advances in mass spectrometry-enabled multiomics at single-cell resolution

Kumar, Rashmi; Zemaitis, Kevin; Fulcher, James M.; Pasa-Tolic, Ljiljana

doi:10.1016/j.copbio.2024.103096

Title: Advances in mass spectrometry-enabled multiomics at single-cell resolution

Journal Article · 01 March 2024 · Current Opinion in Biotechnology

DOI: https://doi.org/10.1016/j.copbio.2024.103096 · OSTI ID:2322504

Kumar, Rashmi ^[1];

^[1]; Fulcher, James M. ^[1]; Pasa-Tolic, Ljiljana ^[1]

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

We report biological organisms are multifaceted, intricate systems where slight perturbations can result in extensive changes in gene expression, protein abundance and/or activity, and metabolic flux. These changes occur at different timescales, spatially across cells of heterogeneous origins, and within single-cells. Hence, multimodal measurements at the smallest biological scales are necessary to capture dynamic changes in heterogeneous biological systems. Of the analytical techniques used to measure biomolecules, mass spectrometry (MS) has proven to be a powerful option due to its sensitivity, robustness, and flexibility with regard to the breadth of biomolecules that can be analyzed. Recently, many studies have coupled MS to other analytical techniques with the goal of measuring multiple modalities from the same single-cell. It is with these concepts in mind that we focus this review on MS-enabled multiomic measurements at single-cell or near-single- cell resolution.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 2322504

Report Number(s):: PNNL-SA--194867

Journal Information:: Current Opinion in Biotechnology, Journal Name: Current Opinion in Biotechnology Vol. 87; ISSN 0958-1669

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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47 OTHER INSTRUMENTATION
multi-omics
single cell
genomics
transcriptomics
proteomics
lipidomics
metabolomics
imaging mass spectrometry
spatial analysis

Title: Advances in mass spectrometry-enabled multiomics at single-cell resolution

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References (48)

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