An Engineered Laccase from Fomitiporia mediterranea Accelerates Lignocellulose Degradation

Pham, Le Thanh Mai; Deng, Kai; Choudhary, Hemant; Northen, Trent R.; Singer, Steven W.; Adams, Paul D.; Simmons, Blake A.; Sale, Kenneth L.

doi:10.3390/biom14030324

Title: An Engineered Laccase from Fomitiporia mediterranea Accelerates Lignocellulose Degradation

Journal Article · 08 March 2024 · Biomolecules

DOI: https://doi.org/10.3390/biom14030324 · OSTI ID:2320293

Pham, Le Thanh Mai ^[1]; Deng, Kai ^[1];

^[1]; Northen, Trent R. ^[2]; Singer, Steven W. ^[2]; Adams, Paul D. ^[3];

^[2];

^[4]

Sandia National Laboratories (SNL-CA), Livermore, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Berkeley, CA (United States)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)

Laccases from white-rot fungi catalyze lignin depolymerization, a critical first step to upgrading lignin to valuable biodiesel fuels and chemicals. In this study, a wildtype laccase from the basidiomycete Fomitiporia mediterranea (Fom_lac) and a variant engineered to have a carbohydrate-binding module (Fom_CBM) were studied for their ability to catalyze cleavage of β-O-4' ether and C–C bonds in phenolic and non-phenolic lignin dimers using a nanostructure-initiator mass spectrometry-based assay. Fom_lac and Fom_CBM catalyze β-O-4' ether and C–C bond breaking, with higher activity under acidic conditions (pH < 6). The potential of Fom_lac and Fom_CBM to enhance saccharification yields from untreated and ionic liquid pretreated pine was also investigated. Adding Fom_CBM to mixtures of cellulases and hemicellulases improved sugar yields by 140% on untreated pine and 32% on cholinium lysinate pretreated pine when compared to the inclusion of Fom_lac to the same mixtures. Adding either Fom_lac or Fom_CBM to mixtures of cellulases and hemicellulases effectively accelerates enzymatic hydrolysis, demonstrating its potential applications for lignocellulose valorization. We postulate that additional increases in sugar yields for the Fom_CBM enzyme mixtures were due to Fom_CBM being brought more proximal to lignin through binding to either cellulose or lignin itself.

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Research Organization:: Sandia National Lab. (SNL-CA), Livermore, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: NA0003525; AC02-05CH11231

OSTI ID:: 2320293

Alternate ID(s):: OSTI ID: 2320336

Report Number(s):: SAND-2024-02798J

Journal Information:: Biomolecules, Vol. 14, Issue 3; ISSN 2218-273X

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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