RNA oligomers at atomic resolution containing 1‐methylpseudouridine, an essential building block of mRNA vaccines

Nievergelt, Philipp; Berliat, Florian; McAuley, Katherine E.; Dorgan, Colin R.; van Well, Renate Maria; Thorn, Andrea; Spingler, Bernhard

doi:10.1002/cmdc.202300600

Title: RNA oligomers at atomic resolution containing 1‐methylpseudouridine, an essential building block of mRNA vaccines

Journal Article · 29 February 2024 · ChemMedChem

DOI: https://doi.org/10.1002/cmdc.202300600 · OSTI ID:2317671

^[1]; Berliat, Florian ^[1];

^[2]; Dorgan, Colin R. ^[3]; van Well, Renate Maria ^[3];

^[4];

^[1]

Department of Chemistry University of Zurich 8057 Zurich Switzerland
Paul Scherrer Institute 5232 Villigen PSI Switzerland
Biosynth Limited Compton Berkshire RG20 6NE United Kingdom
Institut für Nanostruktur und Festkörperphysik Universität Hamburg 22761 Hamburg Germany

Abstract All widely used mRNA vaccines against COVID‐19 contain in their sequence 1‐methylpseudouridine ( m1Ψ ) instead of uridine. In this publication, we report two high resolution crystal structures (at up to 1.01 and 1.32 Å, respectively) of one such double‐stranded 12‐mer RNA sequence crystallized in two crystal forms. The structures are compared with similar structures which do not contain this modification. Additionally, the X‐ray structure of 1‐methyl‐pseudouridine itself was determined.

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Sponsoring Organization:: USDOE

OSTI ID:: 2317671

Journal Information:: ChemMedChem, Journal Name: ChemMedChem Vol. 19 Journal Issue: 7; ISSN 1860-7179

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: France

Language:: English

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