Overexpression of the Phosphatidylcholine: Diacylglycerol Cholinephosphotransferase (PDCT) gene increases carbon flux toward triacylglycerol (TAG) synthesis in Camelina sativa seeds

Abdullah, Hesham M.; Pang, Na; Chilcoat, Benjamin; Shachar-Hill, Yair; Schnell, Danny J.; Dhankher, Om Parkash

doi:10.1016/j.plaphy.2024.108470

Title: Overexpression of the Phosphatidylcholine: Diacylglycerol Cholinephosphotransferase (PDCT) gene increases carbon flux toward triacylglycerol (TAG) synthesis in Camelina sativa seeds

Journal Article · 01 March 2024 · Plant Physiology and Biochemistry

DOI: https://doi.org/10.1016/j.plaphy.2024.108470 · OSTI ID:2316083

Abdullah, Hesham M.; Pang, Na; Chilcoat, Benjamin;

; Schnell, Danny J.;

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Sponsoring Organization:: USDOE

Grant/Contract Number:: -SC0018269; -AR0000200

OSTI ID:: 2316083

Journal Information:: Plant Physiology and Biochemistry, Journal Name: Plant Physiology and Biochemistry Vol. 208 Journal Issue: C; ISSN 0981-9428

Publisher:: ElsevierCopyright Statement

Country of Publication:: France

Language:: English

References (69)

Redirection of metabolic flux for high levels of omega-7 monounsaturated fatty acid accumulation in camelina seeds Nguyen, Huu Tam; Park, Hyunwoo; Koster, Karen L. Plant Biotechnology Journal, Vol. 13, Issue 1 https://doi.org/10.1111/pbi.12233	journal	July 2014
Probing in Vivo Metabolism by Stable Isotope Labeling of Storage Lipids and Proteins in Developing Brassica napus Embryos Schwender, Jörg; Ohlrogge, John B. Plant Physiology, Vol. 130, Issue 1 https://doi.org/10.1104/pp.004275	journal	August 2002
CRISPR/Cas9-Induced fad2 and rod1 Mutations Stacked With fae1 Confer High Oleic Acid Seed Oil in Pennycress (Thlaspi arvense L.) Jarvis, Brice A.; Romsdahl, Trevor B.; McGinn, Michaela G. Frontiers in Plant Science, Vol. 12 https://doi.org/10.3389/fpls.2021.652319	journal	April 2021
Dietary supplementation with seed oil from transgenicCamelina sativainduces similar increments in plasma and erythrocyte DHA and EPA to fish oil in healthy humans West, Annette L.; Miles, Elizabeth A.; Lillycrop, Karen A. British Journal of Nutrition, Vol. 124, Issue 9 https://doi.org/10.1017/S0007114520002044	journal	June 2020
Alterations of flower fertility, plant size, seed weight, and seed oil content in transgenic Camelina sativa plants overexpressing CYP78A Hölzl, Georg; Dörmann, Peter Industrial Crops and Products, Vol. 170 https://doi.org/10.1016/j.indcrop.2021.113794	journal	October 2021
Camelina as a sustainable oilseed crop: Contributions of plant breeding and genetic engineering Vollmann, Johann; Eynck, Christina Biotechnology Journal, Vol. 10, Issue 4 https://doi.org/10.1002/biot.201400200	journal	February 2015
The Phosphatidylcholine Diacylglycerol Cholinephosphotransferase Is Required for Efficient Hydroxy Fatty Acid Accumulation in Transgenic Arabidopsis Hu, Zhaohui; Ren, Zhonghai; Lu, Chaofu Plant Physiology, Vol. 158, Issue 4 https://doi.org/10.1104/pp.111.192153	journal	February 2012
Camelina sativa phosphatidylcholine:diacylglycerol cholinephosphotransferase‐catalyzed interconversion does not discriminate between substrates Demski, Kamil; Jeppson, Simon; Stymne, Sten Lipids, Vol. 56, Issue 6 https://doi.org/10.1002/lipd.12322	journal	August 2021
Analyzing real-time PCR data by the comparative CT method Schmittgen, Thomas D.; Livak, Kenneth J. Nature Protocols, Vol. 3, Issue 6 https://doi.org/10.1038/nprot.2008.73	journal	June 2008
Tracking the metabolic pulse of plant lipid production with isotopic labeling and flux analyses: Past, present and future Allen, Doug K.; Bates, Philip D.; Tjellström, Henrik Progress in Lipid Research, Vol. 58 https://doi.org/10.1016/j.plipres.2015.02.002	journal	April 2015
Increasing the flow of carbon into seed oil Weselake, Randall J.; Taylor, David C.; Rahman, M. Habibur Biotechnology Advances, Vol. 27, Issue 6 https://doi.org/10.1016/j.biotechadv.2009.07.001	journal	November 2009
Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter Vigeolas, Helene; Waldeck, Peter; Zank, Thorsten Plant Biotechnology Journal, Vol. 5, Issue 3 https://doi.org/10.1111/j.1467-7652.2007.00252.x	journal	May 2007
Seed-specific suppression of ADP-glucose pyrophosphorylase in Camelina sativa increases seed size and weight Na, GunNam; Aryal, Niranjan; Fatihi, Abdelhak Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1334-2	journal	December 2018
Constitutive or seed-specific overexpression of Arabidopsis G-protein γ subunit 3 ( AGG3 ) results in increased seed and oil production and improved stress tolerance in Camelina sativa Roy Choudhury, Swarup; Riesselman, Adam J.; Pandey, Sona Plant Biotechnology Journal, Vol. 12, Issue 1 https://doi.org/10.1111/pbi.12115	journal	September 2013
Camelina, a Swiss knife for plant lipid biotechnology Faure, Jean-Denis; Tepfer, Mark OCL, Vol. 23, Issue 5 https://doi.org/10.1051/ocl/2016023	journal	June 2016
Tailoring the composition of novel wax esters in the seeds of transgenic Camelina sativa through systematic metabolic engineering Ruiz-Lopez, Noemi; Broughton, Richard; Usher, Sarah Plant Biotechnology Journal, Vol. 15, Issue 7 https://doi.org/10.1111/pbi.12679	journal	February 2017
A transgene design for enhancing oil content in Arabidopsis and Camelina seeds Zhu, Yerong; Xie, Linan; Chen, Grace Q. Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1049-4	journal	February 2018
Compartmentation of Triacylglycerol Accumulation in Plants Chapman, Kent D.; Ohlrogge, John B. Journal of Biological Chemistry, Vol. 287, Issue 4, p. 2288-2294 https://doi.org/10.1074/jbc.R111.290072	journal	November 2011
Lipid labeling from acetate or glycerol in cultured embryos of Camelina sativa seeds: A tale of two substrates Pollard, Mike; Delamarter, Danielle; Martin, Tina M. Phytochemistry, Vol. 118 https://doi.org/10.1016/j.phytochem.2015.07.021	journal	October 2015
A Transgenic Camelina sativa Seed Oil Effectively Replaces Fish Oil as a Dietary Source of Eicosapentaenoic Acid in Mice Tejera, Noemi; Vauzour, David; Betancor, Monica B. The Journal of Nutrition, Vol. 146, Issue 2 https://doi.org/10.3945/jn.115.223941	journal	January 2016
Over-expression of AtPAP2 in Camelina sativa leads to faster plant growth and higher seed yield Zhang, Youjun; Yu, Laura; Yung, Ka-Fu Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-19	journal	January 2012
A Phospholipase C-Like Protein From Ricinus communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina sativa Aryal, Niranjan; Lu, Chaofu Frontiers in Plant Science, Vol. 9 https://doi.org/10.3389/fpls.2018.01576	journal	November 2018
The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene Zou, Jitao; Wei, Yangdou; Jako, Colette The Plant Journal, Vol. 19, Issue 6 https://doi.org/10.1046/j.1365-313x.1999.00555.x	journal	September 1999
Overexpression of Arabidopsis WRI1 enhanced seed mass and storage oil content in Camelina sativa An, Dahee; Suh, Mi Chung Plant Biotechnology Reports, Vol. 9, Issue 3 https://doi.org/10.1007/s11816-015-0351-x	journal	May 2015
Identification of multiple lipid genes with modifications in expression and sequence associated with the evolution of hydroxy fatty acid accumulation in Physaria fendleri Horn, Patrick J.; Liu, Jinjie; Cocuron, Jean-Christophe The Plant Journal, Vol. 86, Issue 4 https://doi.org/10.1111/tpj.13163	journal	May 2016
Transcriptome profiling of Camelina sativa to identify genes involved in triacylglycerol biosynthesis and accumulation in the developing seeds Abdullah, Hesham M.; Akbari, Parisa; Paulose, Bibin Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0555-5	journal	July 2016
Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds Wickramarathna, Aruna D.; Siloto, Rodrigo M. P.; Mietkiewska, Elzbieta BMC Biotechnology, Vol. 15, Issue 1 https://doi.org/10.1186/s12896-015-0156-6	journal	June 2015
Development of camelina enhanced with drought stress resistance and seed oil production by co-overexpression of MYB96A and DGAT1C Kim, Ryeo Jin; Kim, Hyun Uk; Suh, Mi Chung Industrial Crops and Products, Vol. 138 https://doi.org/10.1016/j.indcrop.2019.111475	journal	October 2019
Camelina oil as a fuel for diesel transport engines Bernardo, Aurore; Howard-Hildige, Robin; O'Connell, Adrian Industrial Crops and Products, Vol. 17, Issue 3 https://doi.org/10.1016/S0926-6690(02)00098-5	journal	May 2003
Cloning and Functional Characterization of a Phospholipid:Diacylglycerol Acyltransferase from Arabidopsis Ståhl, Ulf; Carlsson, Anders S.; Lenman, Marit Plant Physiology, Vol. 135, Issue 3 https://doi.org/10.1104/pp.104.044354	journal	July 2004
Camelina (Camelina sativa L.) oil as a biofuels feedstock: Golden opportunity or false hope? Moser, Bryan R. Lipid Technology, Vol. 22, Issue 12 https://doi.org/10.1002/lite.201000068	journal	December 2010
Multigene Engineering of Triacylglycerol Metabolism Boosts Seed Oil Content in Arabidopsis van Erp, Harrie; Kelly, Amélie A.; Menard, Guillaume Plant Physiology, Vol. 165, Issue 1 https://doi.org/10.1104/pp.114.236430	journal	April 2014
Expression of a high‐activity diacylglycerol acetyltransferase results in enhanced synthesis of acetyl‐TAG in camelina seed oil Alkotami, Linah; Kornacki, Catherine; Campbell, Shahna The Plant Journal, Vol. 106, Issue 4 https://doi.org/10.1111/tpj.15210	journal	March 2021
Acyl-Lipid Metabolism Li-Beisson, Yonghua; Shorrosh, Basil; Beisson, Fred The Arabidopsis Book, Vol. 11 https://doi.org/10.1199/tab.0161	journal	January 2013
Engineering Camelina sativa (L.) Crantz for enhanced oil and seed yields by combining diacylglycerol acyltransferase1 and glycerol-3-phosphate dehydrogenase expression Chhikara, Sudesh; Abdullah, Hesham M.; Akbari, Parisa Plant Biotechnology Journal, Vol. 16, Issue 5 https://doi.org/10.1111/pbi.12847	journal	November 2017
Metabolic engineering of oilseed crops to produce high levels of novel acetyl glyceride oils with reduced viscosity, freezing point and calorific value Liu, Jinjie; Rice, Adam; McGlew, Kathleen Plant Biotechnology Journal, Vol. 13, Issue 6 https://doi.org/10.1111/pbi.12325	journal	March 2015
An improved method for rapid analysis of the fatty acids of glycerolipids Ichihara, Ken’ichi; Shibahara, Akira; Yamamoto, Kohei Lipids, Vol. 31, Issue 5 https://doi.org/10.1007/BF02522648	journal	May 1996
Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight Jako, Colette; Kumar, Arvind; Wei, Yangdou Plant Physiology, Vol. 126, Issue 2 https://doi.org/10.1104/pp.126.2.861	journal	June 2001
Molecular modification of triacylglycerol accumulation by over-expression of DGAT1 to produce canola with increased seed oil content under field conditionsThis paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute. Taylor, David C.; Zhang, Yan; Kumar, Arvind Botany, Vol. 87, Issue 6 https://doi.org/10.1139/B08-101	journal	June 2009
Characterization of the morphological changes and fatty acid profile of developing Camelina sativa seeds Rodríguez-Rodríguez, Manuel Fernando; Sánchez-García, Alicia; Salas, Joaquín J. Industrial Crops and Products, Vol. 50 https://doi.org/10.1016/j.indcrop.2013.07.042	journal	October 2013
Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenic Camelina sativa expressing diacylglycerol acyltransferase 1 and glycerol-3-phosphate dehydrogenase Abdullah, Hesham M.; Chhikara, Sudesh; Akbari, Parisa Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1326-2	journal	December 2018
Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase: Plant cDNAs encoding diacylglycerol acyltransferase Bouvier-Navé, Pierrette; Benveniste, Pierre; Oelkers, Peter European Journal of Biochemistry, Vol. 267, Issue 1 https://doi.org/10.1046/j.1432-1327.2000.00961.x	journal	January 2000
High level accumulation of EPA and DHA in field‐grown transgenic Camelina – a multi‐territory evaluation of TAG accumulation and heterogeneity Han, Lihua; Usher, Sarah; Sandgrind, Sjur Plant Biotechnology Journal, Vol. 18, Issue 11 https://doi.org/10.1111/pbi.13385	journal	May 2020
Production of mono- and sesquiterpenes in Camelina sativa oilseed Augustin, Jörg M.; Higashi, Yasuhiro; Feng, Xiaohong Planta, Vol. 242, Issue 3, p. 693-708 https://doi.org/10.1007/s00425-015-2367-4	journal	July 2015
Signaling phospholipids in plant development: small couriers determining cell fate Champeyroux, Chloe; Stoof, Claudia; Rodriguez-Villalon, Antia Current Opinion in Plant Biology, Vol. 57 https://doi.org/10.1016/j.pbi.2020.05.007	journal	October 2020
The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure Kagale, Sateesh; Koh, Chushin; Nixon, John Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms4706	journal	April 2014
Camelina-derived jet fuel and diesel: Sustainable advanced biofuels Shonnard, David R.; Williams, Larry; Kalnes, Tom N. Environmental Progress & Sustainable Energy, Vol. 29, Issue 3 https://doi.org/10.1002/ep.10461	journal	June 2010
Combinatorial Effects of Fatty Acid Elongase Enzymes on Nervonic Acid Production in Camelina sativa Huai, Dongxin; Zhang, Yuanyuan; Zhang, Chunyu PLOS ONE, Vol. 10, Issue 6 https://doi.org/10.1371/journal.pone.0131755	journal	June 2015
The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase Routaboul, Jean-Marc; Benning, Christoph; Bechtold, Nicole Plant Physiology and Biochemistry, Vol. 37, Issue 11, p. 831-840 https://doi.org/10.1016/S0981-9428(99)00115-1	journal	November 1999
Biochemical pathways in seed oil synthesis Bates, Philip D.; Stymne, Sten; Ohlrogge, John Current Opinion in Plant Biology, Vol. 16, Issue 3 https://doi.org/10.1016/j.pbi.2013.02.015	journal	June 2013
Effect of overexpression of LPAAT and GPD1 on lipid synthesis and composition in green microalga Chlamydomonas reinhardtii Wang, Chaogang; Li, Yi; Lu, Jun Journal of Applied Phycology, Vol. 30, Issue 3 https://doi.org/10.1007/s10811-017-1349-2	journal	December 2017
Improving seed size, seed weight and seedling emergence in Camelina sativa by overexpressing the Atsob3-6 gene variant Sharma Koirala, Pushpa; Neff, Michael M. Transgenic Research, Vol. 29, Issue 4 https://doi.org/10.1007/s11248-020-00208-9	journal	August 2020
Module-specific regulation of the β-phaseolin promoter during embryogenesis Chandrasekharan, Mahesh B.; Bishop, Kenneth J.; Hall, Timothy C. The Plant Journal, Vol. 33, Issue 5 https://doi.org/10.1046/j.1365-313X.2003.01678.x	journal	February 2003
Phosphatidylcholine and its relationship to triacylglycerol biosynthesis in oil-tissues Griffiths, G.; Stymne, S.; Stobart, A. K. Phytochemistry, Vol. 27, Issue 7 https://doi.org/10.1016/0031-9422(88)80102-X	journal	January 1988
Analysis of Acyl Fluxes through Multiple Pathways of Triacylglycerol Synthesis in Developing Soybean Embryos Bates, Philip D.; Durrett, Timothy P.; Ohlrogge, John B. Plant Physiology, Vol. 150, Issue 1 https://doi.org/10.1104/pp.109.137737	journal	March 2009
Transgenic camelina oil is an effective source of eicosapentaenoic acid and docosahexaenoic acid in diets for farmed rainbow trout, in terms of growth, tissue fatty acid content, and fillet sensory properties Osmond, Angelisa T. Y.; Arts, Michael T.; Bazinet, Richard P. Journal of the World Aquaculture Society, Vol. 52, Issue 4 https://doi.org/10.1111/jwas.12788	journal	May 2021
Metabolic Engineering a Model Oilseed Camelina sativa for the Sustainable Production of High-Value Designed Oils Yuan, Lixia; Li, Runzhi Frontiers in Plant Science, Vol. 11 https://doi.org/10.3389/fpls.2020.00011	journal	February 2020
Acyl Editing and Headgroup Exchange Are the Major Mechanisms That Direct Polyunsaturated Fatty Acid Flux into Triacylglycerols Bates, Philip D.; Fatihi, Abdelhak; Snapp, Anna R. Plant Physiology, Vol. 160, Issue 3 https://doi.org/10.1104/pp.112.204438	journal	August 2012
Functional analysis of diacylglycerol acyltransferase1 genes from Camelina sativa and effects of CsDGAT1B overexpression on seed mass and storage oil content in C. sativa Kim, Hyojin; Park, Ji Hyun; Kim, Da Jung Plant Biotechnology Reports, Vol. 10, Issue 3 https://doi.org/10.1007/s11816-016-0394-7	journal	May 2016
The biochemistry of headgroup exchange during triacylglycerol synthesis in canola Bai, Shuangyi; Wallis, James G.; Denolf, Peter The Plant Journal, Vol. 103, Issue 1 https://doi.org/10.1111/tpj.14709	journal	February 2020
Lipid extraction of tissues with a low-toxicity solvent Hara, Atsushi; Radin, Norman S. Analytical Biochemistry, Vol. 90, Issue 1 https://doi.org/10.1016/0003-2697(78)90046-5	journal	October 1978
Expression of a Lychee PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE with an Escherichia coli CYCLOPROPANE SYNTHASE Enhances Cyclopropane Fatty Acid Accumulation in Camelina Seeds Yu, Xiao-Hong; Cai, Yuanheng; Chai, Jin Plant Physiology, Vol. 180, Issue 3 https://doi.org/10.1104/pp.19.00396	journal	May 2019
Phospholipase Dζ Enhances Diacylglycerol Flux into Triacylglycerol Yang, Wenyu; Wang, Geliang; Li, Jia Plant Physiology, Vol. 174, Issue 1 https://doi.org/10.1104/pp.17.00026	journal	March 2017
Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation Lu, Chaofu; Kang, Jinling Plant Cell Reports, Vol. 27, Issue 2 https://doi.org/10.1007/s00299-007-0454-0	journal	September 2007
Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa Usher, Sarah; Han, Lihua; Haslam, Richard P. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-06838-0	journal	July 2017
A fatty acid condensing enzyme from Physaria fendleri increases hydroxy fatty acid accumulation in transgenic oilseeds of Camelina sativa Snapp, Anna R.; Kang, Jinling; Qi, Xiaoli Planta, Vol. 240, Issue 3 https://doi.org/10.1007/s00425-014-2122-2	journal	July 2014
Function and regulation of phospholipid signalling in plants Xue, Hong-Wei; Chen, Xu; Mei, Yu Biochemical Journal, Vol. 421, Issue 2 https://doi.org/10.1042/BJ20090300	journal	June 2009
Understanding the control of acyl flux through the lipid metabolic network of plant oil biosynthesis Bates, Philip D. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1861, Issue 9 https://doi.org/10.1016/j.bbalip.2016.03.021	journal	September 2016
An enzyme regulating triacylglycerol composition is encoded by the ROD1 gene of Arabidopsis Lu, C.; Xin, Z.; Ren, Z. Proceedings of the National Academy of Sciences, Vol. 106, Issue 44 https://doi.org/10.1073/pnas.0908848106	journal	October 2009

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