Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria

Sebesta, Jacob; Cantrell, Michael; Schaedig, Eric; Hou, Harvey J. M.; Pastore, Colleen; Chou, Katherine J.; Xiong, Wei; Guarnieri, Michael T.; Yu, Jianping

doi:10.3389/fpls.2024.1342496

Title: Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria

Journal Article · 06 February 2024 · Frontiers in Plant Science

DOI: https://doi.org/10.3389/fpls.2024.1342496 · OSTI ID:2290435

Sebesta, Jacob ^[1]; Cantrell, Michael ^[1]; Schaedig, Eric ^[1]; Hou, Harvey J. M. ^[2]; Pastore, Colleen ^[1]; Chou, Katherine J. ^[1]; Xiong, Wei ^[1]; Guarnieri, Michael T. ^[1]; Yu, Jianping ^[1]

National Renewable Energy Laboratory (NREL), Golden, CO (United States)
National Renewable Energy Laboratory (NREL), Golden, CO (United States); Alabama State Univ., Montgomery, AL (United States)

Identification and manipulation of cellular energy regulation mechanisms may be a strategy to increase productivity in photosynthetic organisms. This work tests the hypothesis that polyphosphate synthesis and degradation play a role in energy management by storing or dissipating energy in the form of ATP. A polyphosphate kinase (ppk) knock-out strain unable to synthesize polyphosphate was generated in the cyanobacterium Synechocystis sp. PCC 6803. This mutant strain demonstrated higher ATP levels and faster growth than the wildtype strain in high-carbon conditions and had a growth defect under multiple stress conditions. In a strain that combined ppk deletion with heterologous expression of ethylene-forming enzyme, higher ethylene productivity was observed than in the wildtype background. These results support the role of polyphosphate synthesis and degradation as an energy regulation mechanism and suggest that such mechanisms may be effective targets in biocontainment design.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 2290435

Alternate ID(s):: OSTI ID: 2325025

Report Number(s):: NREL/JA-2700-87881; MainId:88656; UUID:e5b2e3c2-bb16-46ac-93fd-ade584ed46fe; MainAdminId:72082

Journal Information:: Frontiers in Plant Science, Vol. 15; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
biocontainment
cyanobacteria
energy regulation
ethylene
polyphosphate

Title: Polyphosphate kinase deletion increases laboratory productivity in cyanobacteria

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