Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall

Poulhazan, Alexandre; Arnold, Alexandre A.; Mentink-Vigier, Frederic; Muszyński, Artur; Azadi, Parastoo; Halim, Adnan; Vakhrushev, Sergey Y.; Joshi, Hiren Jitendra; Wang, Tuo; Warschawski, Dror E.; Marcotte, Isabelle

doi:10.1038/s41467-024-45246-7

Title: Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall

Journal Article · 01 February 2024 · Nature Communications

DOI: https://doi.org/10.1038/s41467-024-45246-7 · OSTI ID:2283780

Poulhazan, Alexandre; Arnold, Alexandre A.;

;

; Azadi, Parastoo; Halim, Adnan;

;

Abstract Microalgae are a renewable and promising biomass for large-scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-level model of the non-crystalline and water-insoluble glycoprotein-rich cell wall of Chlamydomonas reinhardtii . Using in situ solid-state and sensitivity-enhanced nuclear magnetic resonance, we reveal unprecedented details on the protein and carbohydrate composition and their nanoscale heterogeneity, as well as the presence of spatially segregated protein- and glycan-rich regions with different dynamics and hydration levels. We show that mannose-rich lower-molecular-weight proteins likely contribute to the cell wall cohesion by binding to high-molecular weight protein components, and that water provides plasticity to the cell-wall architecture. The structural insight exemplifies strategies used by nature to form cell walls devoid of cellulose or other glycan polymers.

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Research Organization:: Michigan State Univ., East Lansing, MI (United States); Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: Centre National de la Recherche Scientifique; Danish National Research Foundation; Mizutani Foundation for Glycoscience; Natural Sciences and Engineering Research Council of Canada (NSERC); Novo Nordisk Foundation; USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Renewable Power Office. Wind Energy Technologies Office; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: SC0015662; SC0023702

OSTI ID:: 2283780

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 15; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Title: Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall

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