Fe protein docking transduces conformational changes to MoFe nitrogenase active site in a nucleotide-dependent manner

Tokmina-Lukaszewska, Monika; Huang, Qi; Berry, Luke; Kallas, Hayden; Peters, John W.; Seefeldt, Lance C.; Raugei, Simone; Bothner, Brian

doi:10.1038/s42004-023-01046-6

Title: Fe protein docking transduces conformational changes to MoFe nitrogenase active site in a nucleotide-dependent manner

Journal Article · 18 November 2023 · Communications Chemistry

DOI: https://doi.org/10.1038/s42004-023-01046-6 · OSTI ID:2283145

Tokmina-Lukaszewska, Monika ^[1]; Huang, Qi ^[2]; Berry, Luke ^[1]; Kallas, Hayden ^[3]; Peters, John W. ^[4];

^[3]; Raugei, Simone ^[2];

^[1]

Montana State Univ., Bozeman, MT (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Utah State Univ., Logan, UT (United States)
Univ. of Oklahoma, Norman, OK (United States)

The reduction of dinitrogen to ammonia catalyzed by nitrogenase involves a complex series of events, including ATP hydrolysis, electron transfer, and activation of metal clusters for N₂ reduction. Early evidence shows that an essential part of the mechanism involves transducing information between the nitrogenase component proteins through conformational dynamics. Here, millisecond time-resolved hydrogen-deuterium exchange mass spectrometry was used to unravel peptide-level protein motion on the time scale of catalysis of Mo-dependent nitrogenase from Azotobacter vinelandii. Normal mode analysis calculations complemented this data, providing insights into the specific signal transduction pathways that relay information across protein interfaces at distances spanning 100 Å. Together, these results show that conformational changes induced by protein docking are rapidly transduced to the active site, suggesting a specific mechanism for activating the metal cofactor in the enzyme active site.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); National Institutes of Health (NIH)

Grant/Contract Number:: AC05-76RL01830; SC0018143; SC0010687; P20GM103474; S10OD28650

OSTI ID:: 2283145

Report Number(s):: PNNL-SA-182764

Journal Information:: Communications Chemistry, Vol. 6, Issue 1; ISSN 2399-3669

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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