Shifts in bacterial traits under chronic nitrogen deposition align with soil processes in arbuscular, but not ectomycorrhizal-associated trees

Piñeiro, Juan; Dang, Chansotheary; Walkup, Jeth G. V.; Kuzniar, Teagan; Winslett, Rachel; Blazewicz, Steven J.; Freedman, Zachary B.; Brzostek, Edward; Morrissey, Ember M.

doi:10.1111/gcb.17030

Title: Shifts in bacterial traits under chronic nitrogen deposition align with soil processes in arbuscular, but not ectomycorrhizal-associated trees

Journal Article · 26 November 2023 · Global Change Biology

DOI: https://doi.org/10.1111/gcb.17030 · OSTI ID:2282841

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West Virginia University, Morgantown, WV (United States); University of Madrid (Spain)
West Virginia University, Morgantown, WV (United States)
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
University of Wisconsin‐Madison, WI (United States)

Nitrogen (N) deposition increases soil carbon (C) storage by reducing microbial activity. These effects vary in soil beneath trees that associate with arbuscular (AM) and ectomycorrhizal (ECM) fungi. Variation in carbon C and N uptake traits among microbes may explain differences in soil nutrient cycling between mycorrhizal associations in response to high N loads, a mechanism not previously examined due to methodological limitations. Here, we used quantitative Stable Isotope Probing (qSIP) to measure bacterial C and N assimilation rates from an added organic compound, which we conceptualize as functional traits. As such, we applied a trait-based approach to explore whether variation in assimilation rates of bacterial taxa can inform shifts in soil function under chronic N deposition. We show taxon-specific and community-wide declines of bacterial C and N uptake under chronic N deposition in both AM and ECM soils. N deposition-induced reductions in microbial activity were mirrored by declines in soil organic matter mineralization rates in AM but not ECM soils. Our findings suggest C and N uptake traits of bacterial communities can predict C cycling feedbacks to N deposition in AM soils, but additional data, for instance on the traits of fungi, may be needed to connect microbial traits with soil C and N cycling in ECM systems. In conclusion, our study also highlights the potential of employing qSIP in conjunction with trait-based analytical approaches to inform how ecological processes of microbial communities influence soil functioning.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); Ministry of Science, Innovation and Universities (MICINN); National Science Foundation (NSF)

Grant/Contract Number:: AC52-07NA27344; SC0019472

OSTI ID:: 2282841

Alternate ID(s):: OSTI ID: 2228337

Report Number(s):: LLNL--JRNL-852816; 1080378

Journal Information:: Global Change Biology, Journal Name: Global Change Biology Journal Issue: 1 Vol. 30; ISSN 1354-1013

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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bacterial traits
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Title: Shifts in bacterial traits under chronic nitrogen deposition align with soil processes in arbuscular, but not ectomycorrhizal-associated trees

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