DNA transfer between two different species mediated by heterologous cell fusion in Clostridium coculture

Charubin, Kamil; Hill, John D.; Papoutsakis, Eleftherios Terry

doi:10.1128/mbio.03133-23

Title: DNA transfer between two different species mediated by heterologous cell fusion in Clostridium coculture

Journal Article · 12 January 2024 · mBio (Online)

DOI: https://doi.org/10.1128/mbio.03133-23 · OSTI ID:2281037

Charubin, Kamil ^[1];

^[1];

^[1]

Univ. of Delaware, Newark, DE (United States)

Prokaryotic evolution is driven by random mutations and horizontal gene transfer (HGT). HGT occurs via transformation, transduction, or conjugation. We have previously shown that in syntrophic cocultures of Clostridium acetobutylicum and Clostridium ljungdahlii, heterologous cell fusion leads to a large-scale exchange of proteins and RNA between the two organisms. Here, we present evidence that heterologous cell fusion facilitates the exchange of DNA between the two organisms. Using selective subculturing, we isolated C. acetobutylicum cells which acquired and integrated into their genome portions of plasmid DNA from a plasmid-carrying C. ljungdahlii strain. Limiting-dilution plating and DNA methylation data based on PacBio Single-Molecule Real Time (SMRT) sequencing support the existence of hybrid C. acetobutylicum/C. ljungdahlii cells. These findings expand our understanding of multi-species microbiomes, their survival strategies, and evolution.

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Research Organization:: Univ. of Delaware, Newark, DE (United States)

Sponsoring Organization:: USDOE Advanced Research Projects Agency - Energy (ARPA-E); US Army Research Office (ARO); National Institutes of Health (NIH); National Institute of General Medical Sciences (NIGMS); National Science Foundation (NSF)

Grant/Contract Number:: AR0001505; W911NF-19-1-0274; P200A210065; S10 OD016361; P20 GM103446; IIA-1301765

OSTI ID:: 2281037

Alternate ID(s):: OSTI ID: 2472240

Journal Information:: mBio (Online), Vol. 15, Issue 2; ISSN 2150-7511

Publisher:: American Society for Microbiology (ASM)Copyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Microbiology
Clostridium ljungdahlii
Clostridium acetobutylicum
syntrophy
heterologous cell fusion
DNA exchange
DNA integration
plasmid DNA

Title: DNA transfer between two different species mediated by heterologous cell fusion in Clostridium coculture

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