Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials

Diviesti, Karla; Russell-Parks, Glory A.; Trewyn, Brian G.; Holz, Richard C.

doi:10.1021/acsenvironau.3c00036

Title: Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials

Journal Article · 01 November 2023 · ACS Environmental Au

DOI: https://doi.org/10.1021/acsenvironau.3c00036 · OSTI ID:2280962

Diviesti, Karla ^[1];

^[1];

^[2];

^[1]

Colorado School of Mines, Golden, CO (United States)
Colorado School of Mines, Golden, CO (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Triazine hydrolase fromArthrobacter aurescens TC1 (TrzN) was successfully immobilized on mesoporous silica nanomaterials (MSNs) for the first time. For both nonfunctionalized MSNs and MSNs functionalized with Zn(II), three pore sizes were evaluated for their ability to immobilize wild-type TrzN: Mobile composition of matter no. 41 (small, 3 nm pores), mesoporous silica nanoparticle material with 10 nm pore diameter (MSN-10) (medium, 6-12 nm pores), and pore-expanded MSN-10 (large, 15-30 nm pores). Of these six TrzN:MSN biomaterials, it was shown that TrzN:MSN-10 was the most active (3.8 +/- 0.4 x 10^-5 U/mg) toward the hydrolysis of a 50 uM atrazine solution at 25 degrees C. The TrzN:MSN-10 biomaterial was then coated in chitosan (TrzN:MSN-10:Chit) as chitosan has been shown to increase stability in extreme conditions such as low/high pH, heat shock, and the presence of organic solvents. TrzN:MSN-10:Chit was shown to be a superior TrzN biomaterial to TrzN:MSN-10 as it exhibited higher activity under all storage conditions, in the presence of 20% MeOH, at low and high pH values, and at elevated temperatures up to 80 degrees C. Finally, the TrzN:MSN-10:Chit biomaterial was shown to be fully active in river water, which establishes it as a functional biomaterial under actual field conditions. A combination of these data indicate that the TrzN:MSN-10:Chit biomaterial exhibited the best overall catalytic profile making it a promising biocatalyst for the bioremediation of atrazine.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE); National Science Foundation (NSF); National Institutes of Health (NIH)

Grant/Contract Number:: AC36-08GO28308; CHE-2003861; 1R15CA271365

OSTI ID:: 2280962

Report Number(s):: NREL/JA-5900-88497; MainId:89276; UUID:68e357e4-e66c-4ec2-98a3-4eddc5464de7; MainAdminID:71498

Journal Information:: ACS Environmental Au, Vol. 3, Issue 6; ISSN 2694-2518

Publisher:: ACS PublicationsCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Atrazine Degradation Using Immobilized Triazine Hydrolase from Arthrobacter aurescens TC1 in Mesoporous Silica Nanomaterials

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