Directing polymorph specific calcium carbonate formation with de novo protein templates

Davila-Hernandez, Fatima A.; Jin, Biao; Pyles, Harley; Zhang, Shuai; Wang, Zheming; Huddy, Timothy F.; Bera, Asim K.; Kang, Alex; Chen, Chun-Long; De Yoreo, James J.; Baker, David

doi:10.1038/s41467-023-43608-1

Title: Directing polymorph specific calcium carbonate formation with de novo protein templates

Journal Article · 14 December 2023 · Nature Communications

DOI: https://doi.org/10.1038/s41467-023-43608-1 · OSTI ID:2274875

Davila-Hernandez, Fatima A. ^[1]; Jin, Biao ^[2]; Pyles, Harley ^[1];

^[2]; Wang, Zheming ^[3]; Huddy, Timothy F. ^[1];

^[1]; Kang, Alex ^[1];

^[2];

^[1]

University of Washington, Seattle, WA (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); University of Washington, Seattle, WA (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Biomolecules modulate inorganic crystallization to generate hierarchically structured biominerals, but the atomic structure of the organic-inorganic interfaces that regulate mineralization remain largely unknown. We hypothesized that heterogeneous nucleation of calcium carbonate could be achieved by a structured flat molecular template that pre-organizes calcium ions on its surface. To test this hypothesis, we design helical repeat proteins (DHRs) displaying regularly spaced carboxylate arrays on their surfaces and find that both protein monomers and protein-C^a2+ supramolecular assemblies directly nucleate nano-calcite with non-natural {110} or {202} faces while vaterite, which forms first in the absence of the proteins, is bypassed. These protein-stabilized nanocrystals then assemble by oriented attachment into calcite mesocrystals. We find further that nanocrystal size and polymorph can be tuned by varying the length and surface chemistry of the designed protein templates. Thus, bio-mineralization can be programmed using de novo protein design, providing a route to next-generation hybrid materials.

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Research Organization:: Univ. of Washington, Seattle, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0019288; AC05-76RL01830; AC02-05CH11231; AC02-06CH11357

OSTI ID:: 2274875

Alternate ID(s):: OSTI ID: 2341501; OSTI ID: 2341502

Report Number(s):: PNNL-SA-186141

Journal Information:: Nature Communications, Vol. 14, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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