Transforming layered 2D mats into multiphasic 3D nanofiber scaffolds with tailored gradient features for tissue regeneration
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- Department of Surgery – Transplant and Mary &, Dick Holland Regenerative Medicine Program College of Medicine University of Nebraska Medical Center Omaha Nebraska USA, Eppley Institute for Research in Cancer and Allied Diseases College of Medicine University of Nebraska Medical Center Omaha Nebraska USA
- Department of Surgery – Transplant and Mary &, Dick Holland Regenerative Medicine Program College of Medicine University of Nebraska Medical Center Omaha Nebraska USA
- Department of Surgery – Transplant and Mary &, Dick Holland Regenerative Medicine Program College of Medicine University of Nebraska Medical Center Omaha Nebraska USA, Department of Mechanical and Materials Engineering University of Nebraska Lincoln Lincoln Nebraska USA
Abstract Multiphasic scaffolds with tailored gradient features hold significant promise for tissue regeneration applications. Herein, this work reports the transformation of two‐dimensional (2D) layered fiber mats into three‐dimensional (3D) multiphasic scaffolds using a ‘solids‐of‐revolution’ inspired gas‐foaming expansion technology. These scaffolds feature precise control over fiber alignment, pore size, and regional structure. Manipulating nanofiber mat layers and Pluronic F127 concentrations allows further customization of pore size and fiber alignment within different scaffold regions. The cellular response to multiphasic scaffolds demonstrates that the number of cells migrated and proliferated onto the scaffolds is mainly dependent on the pore size rather than fiber alignment. In vivo subcutaneous implantation of multiphasic scaffolds to rats reveals substantial cell infiltration, neo tissue formation, collagen deposition, and new vessel formation within scaffolds, greatly surpassing the capabilities of traditional nanofiber mats. Histological examination indicates the importance of optimizing pore size and fiber alignment for the promotion of cell infiltration and tissue regeneration. Overall, these scaffolds have potential applications in tissue modeling, studying tissue‐tissue interactions, interface tissue engineering, and high‐throughput screening for optimized tissue regeneration.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 2263385
- Alternate ID(s):
- OSTI ID: 2263386
- Journal Information:
- BMEMat, Journal Name: BMEMat Vol. 2 Journal Issue: 1; ISSN 2751-7438
- Publisher:
- Wiley Blackwell (John Wiley & Sons)Copyright Statement
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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