A large sequenced mutant library – valuable reverse genetic resource that covers 98% of sorghum genes

Jiao, Yinping; Nigam, Deepti; Barry, Kerrie; Daum, Chris; Yoshinaga, Yuko; Lipzen, Anna; Khan, Adil; Parasa, Sai‐Praneeth; Wei, Sharon; Lu, Zhenyuan; Tello‐Ruiz, Marcela K.; Dhiman, Pallavi; Burow, Gloria; Hayes, Chad; Chen, Junping; Brandizzi, Federica; Mortimer, Jenny; Ware, Doreen; Xin, Zhanguo

doi:10.1111/tpj.16582

Title: A large sequenced mutant library – valuable reverse genetic resource that covers 98% of sorghum genes

Journal Article · 14 December 2023 · The Plant Journal

DOI: https://doi.org/10.1111/tpj.16582 · OSTI ID:2234311

^[1]; Nigam, Deepti ^[1]; Barry, Kerrie ^[2]; Daum, Chris ^[2]; Yoshinaga, Yuko ^[2]; Lipzen, Anna ^[2]; Khan, Adil ^[1]; Parasa, Sai‐Praneeth ^[1]; Wei, Sharon ^[3]; Lu, Zhenyuan ^[3]; Tello‐Ruiz, Marcela K. ^[3]; Dhiman, Pallavi ^[1]; Burow, Gloria ^[4]; Hayes, Chad ^[4]; Chen, Junping ^[4];

^[5]; Mortimer, Jenny ^[6];

^[7]; Xin, Zhanguo ^[4]

Department of Plant and Soil Science, Institute of Genomics for Crop Abiotic Stress Tolerance Texas Tech University Lubbock Texas 79409 USA
DOE Joint Genome Institute Lawrence Berkeley National Laboratory Berkeley California 94720 USA
Cold Spring Harbor Laboratory Cold Spring Harbor New York 11724 USA
Plant Stress and Germplasm Development Unit, Crop Systems Research Laboratory USDA‐ARS 3810, 4th Street Lubbock Texas 79424 USA
MSU‐DOE Plant Research Lab Michigan State University East Lansing Michigan USA, Great Lakes Bioenergy Research Center Michigan State University East Lansing Michigan USA, Department of Plant Biology Michigan State University East Lansing Michigan USA
Joint BioEnergy Institute Emeryville California 94608 USA, Environmental Genomics and Systems Biology Division Lawrence Berkeley National Laboratory 1 Cyclotron Road Berkeley California 94720 USA, School of Agriculture, Food and Wine, Waite Research Institute, Waite Research Precinct University of Adelaide Glen Osmond South Australia 5064 Australia
Cold Spring Harbor Laboratory Cold Spring Harbor New York 11724 USA, USDA‐ARS NAA Robert W. Holley Center for Agriculture and Health Agricultural Research Service Ithaca New York 14853 USA

SUMMARY Mutant populations are crucial for functional genomics and discovering novel traits for crop breeding. Sorghum , a drought and heat‐tolerant C4 species, requires a vast, large‐scale, annotated, and sequenced mutant resource to enhance crop improvement through functional genomics research. Here, we report a sorghum large‐scale sequenced mutant population with 9.5 million ethyl methane sulfonate (EMS)‐induced mutations that covered 98% of sorghum's annotated genes using inbred line BTx623. Remarkably, a total of 610 320 mutations within the promoter and enhancer regions of 18 000 and 11 790 genes, respectively, can be leveraged for novel research of cis ‐regulatory elements. A comparison of the distribution of mutations in the large‐scale mutant library and sorghum association panel (SAP) provides insights into the influence of selection. EMS‐induced mutations appeared to be random across different regions of the genome without significant enrichment in different sections of a gene, including the 5′ UTR, gene body, and 3′‐UTR. In contrast, there were low variation density in the coding and UTR regions in the SAP. Based on the K _a / K _s value, the mutant library (~1) experienced little selection, unlike the SAP (0.40), which has been strongly selected through breeding. All mutation data are publicly searchable through SorbMutDB ( https://www.depts.ttu.edu/igcast/sorbmutdb.php ) and SorghumBase ( https://sorghumbase.org/ ). This current large‐scale sequence‐indexed sorghum mutant population is a crucial resource that enriched the sorghum gene pool with novel diversity and a highly valuable tool for the Poaceae family, that will advance plant biology research and crop breeding.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDA; USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; SC0018409

OSTI ID:: 2234311

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Journal Issue: 5 Vol. 117; ISSN 0960-7412

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
ethyl methyl sulfone mutants
resequencing
sorghum

Title: A large sequenced mutant library – valuable reverse genetic resource that covers 98% of sorghum genes

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