Watershed-scale liming reveals the short- and long- term effects of pH on the forest soil microbiome and carbon cycling

Sridhar, Bhavya; Lawrence, Gregory B.; Debenport, Spencer J.; Fahey, Timothy J.; Buckley, Daniel H.; Wilhelm, Roland C.; Goodale, Christine L.

doi:10.1111/1462-2920.16119

Title: Watershed-scale liming reveals the short- and long- term effects of pH on the forest soil microbiome and carbon cycling

Journal Article · 07 July 2022 · Environmental Microbiology

DOI: https://doi.org/10.1111/1462-2920.16119 · OSTI ID:2228481

Sridhar, Bhavya ^[1]; Lawrence, Gregory B. ^[2]; Debenport, Spencer J. ^[3]; Fahey, Timothy J. ^[3];

^[3];

^[3]; Goodale, Christine L. ^[3]

Cornell Univ., Ithaca, NY (United States); Cornell University
US Geological Survey, Troy, NY (United States)
Cornell Univ., Ithaca, NY (United States)

Soil microbial community composition routinely correlates with pH, reflecting both direct pH effects on microbial physiology and long-term biogeochemical feedbacks. For this work, we used two watershed-scale liming experiments to identify short- (2 years) and long-term (25 years) changes in the structure and function of bacterial and fungal communities in organic horizons (O_eand O_a) of acid forest soils. Liming increased soil pH, extractable calcium, and soil carbon stocks, reduced biomass-specific respiration, and caused major changes in the soil microbiome in the short and long term. More taxa responded to liming in the short term (70%) than in the long term (30%), with most showing consistent directional responses at both sites. The ratio of change in relative abundance between limed and reference sites was twofold higher at the long than the short-term site, indicating that the effects of liming grew over time. Liming impacts were most pronounced in fungi, as steep declines of dominant ectomycorrhizal fungi (CenococcumandRussula) occurred at both sites. Liming favoured neutrophilic bacteria over acidophilic populations according to estimated environmental pH optima. Collectively, these results demonstrate that a liming-induced change of one pH unit has an immediate and persistent effect on the structure and function of microbial communities in acid forest soils. The corresponding suppression of respiration indicates that anthropogenic alterations of soil pH, as driven by acid deposition or liming, can affect forest floor C stocks due to pH-driven shifts in community structure.

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Research Organization:: Cornell Univ., Ithaca, NY (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science (BSS)

Grant/Contract Number:: SC0016364

OSTI ID:: 2228481

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 12 Vol. 24; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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54 ENVIRONMENTAL SCIENCES
carbon cycle
forest soil
liming
microbial ecology
soil pH

Title: Watershed-scale liming reveals the short- and long- term effects of pH on the forest soil microbiome and carbon cycling

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