The role of photorespiration in preventing feedback regulation via ATP synthase in Nicotiana tabacum

Smith, Kaila; Strand, Deserah D.; Kramer, David M.; Walker, Berkley J.

doi:10.1111/pce.14759

Title: The role of photorespiration in preventing feedback regulation via ATP synthase in Nicotiana tabacum

Journal Article · 07 November 2023 · Plant, Cell and Environment

DOI: https://doi.org/10.1111/pce.14759 · OSTI ID:2205113

Smith, Kaila ^[1]; Strand, Deserah D. ^[2];

^[3];

^[2]

Department of Energy‐Plant Research Laboratory Michigan State University East Lansing Michigan USA, Department of Plant Biology Michigan State University East Lansing Michigan USA, Plant Biotechnology for Health and Sustainability Program Michigan State University East Lansing Michigan USA
Department of Energy‐Plant Research Laboratory Michigan State University East Lansing Michigan USA, Department of Plant Biology Michigan State University East Lansing Michigan USA
Department of Energy‐Plant Research Laboratory Michigan State University East Lansing Michigan USA, Department of Biochemistry and Molecular Biology Michigan State University East Lansing Michigan USA

Abstract Photorespiration consumes substantial amounts of energy in the forms of adenosine triphosphate (ATP) and reductant making the pathway an important component in leaf energetics. Because of this high reductant demand, photorespiration is proposed to act as a photoprotective electron sink. However, photorespiration consumes more ATP relative to reductant than the C3 cycle meaning increased flux disproportionally increases ATP demand relative to reductant. Here we explore how energetic consumption from photorespiration impacts the flexibility of the light reactions in nicotiana tabacum . Specifically, we demonstrate that decreased photosynthetic efficiency (ϕ _II ) at low photorespiratory flux was related to feedback regulation at the chloroplast ATP synthase. Additionally, decreased ϕ _II at high photorespiratory flux resulted in the accumulation of photoinhibition at photosystem II centers. These results are contrary to the proposed role of photorespiration as a photoprotective electron sink. Instead, our results suggest a novel role of ATP consumption from photorespiration in maintaining ATP synthase activity, with implications for maintaining energy balance and preventing photodamage that will be critical for plant engineering strategies.

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Sponsoring Organization:: USDOE

OSTI ID:: 2205113

Alternate ID(s):: OSTI ID: 2274978

Journal Information:: Plant, Cell and Environment, Journal Name: Plant, Cell and Environment Journal Issue: 2 Vol. 47; ISSN 0140-7791

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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