High-throughput functional trait testing for bacterial pathogens

Stromberg, Zachary R.; Phillips, Shelby M. B.; Omberg, Kristin M.; Hess, Becky M.

doi:10.1128/msphere.00315-23

Title: High-throughput functional trait testing for bacterial pathogens

Journal Article · 12 September 2023 · mSphere

DOI: https://doi.org/10.1128/msphere.00315-23 · OSTI ID:2204722

^[1]; Phillips, Shelby M. B. ^[1]; Omberg, Kristin M. ^[1];

^[1]

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Functional traits are characteristics that affect the fitness and metabolic function of a microorganism. There is growing interest in using high-throughput methods to characterize bacterial pathogens based on functional virulence traits. Traditional methods that phenotype a single organism for a single virulence trait can be time consuming and labor intensive. Alternatively, machine learning of whole-genome sequences (WGS) has shown some success in predicting virulence. However, relying solely on WGS can miss functional traits, particularly for organisms lacking classical virulence factors. We propose that high-throughput assays for functional virulence trait identification should become a prominent method of characterizing bacterial pathogens on a population scale. This work is critical as we move from compiling lists of bacterial species associated with disease to pathogen-agnostic approaches capable of detecting novel microbes. We discuss six key areas of functional trait testing and how advancing high-throughput methods could provide a greater understanding of pathogens.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 2204722

Report Number(s):: PNNL-SA--179628

Journal Information:: mSphere, Journal Name: mSphere Journal Issue: 5 Vol. 8; ISSN 2379-5042

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (84)

Molecular Koch's postulates applied to bacterial pathogenicity — a personal recollection 15 years later Falkow, Stanley Nature Reviews Microbiology, Vol. 2, Issue 1 https://doi.org/10.1038/nrmicro799	journal	January 2004
A High-Throughput Interbacterial Competition Screen Identifies ClpAP in Enhancing Recipient Susceptibility to Type VI Secretion System-Mediated Attack by Agrobacterium tumefaciens Lin, Hsiao-Han; Yu, Manda; Sriramoju, Manoj Kumar Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.03077	journal	February 2020
Antimicrobial Resistance and Virulence: a Successful or Deleterious Association in the Bacterial World? Beceiro, A.; Tomas, M.; Bou, G. Clinical Microbiology Reviews, Vol. 26, Issue 2 https://doi.org/10.1128/CMR.00059-12	journal	April 2013
Altruism of Shiga toxin-producing Escherichia coli: recent hypothesis versus experimental results Łoś, Joanna M.; Łoś, Marcin; Węgrzyn, Alicja Frontiers in Cellular and Infection Microbiology, Vol. 2 https://doi.org/10.3389/fcimb.2012.00166	journal	January 2013
The Next-Generation Sequencing Revolution and Its Impact on Genomics Koboldt, Daniel C.; Steinberg, Karyn Meltz; Larson, David E. Cell, Vol. 155, Issue 1 https://doi.org/10.1016/j.cell.2013.09.006	journal	September 2013
Tracking bacterial lineages in complex and dynamic environments with applications for growth control and persistence Bakshi, Somenath; Leoncini, Emanuele; Baker, Charles Nature Microbiology, Vol. 6, Issue 6 https://doi.org/10.1038/s41564-021-00900-4	journal	May 2021
High-throughput electrophysiological assays for voltage gated ion channels using SyncroPatch 768PE Li, Tianbo; Lu, Gang; Chiang, Eugene Y. PLOS ONE, Vol. 12, Issue 7 https://doi.org/10.1371/journal.pone.0180154	journal	July 2017
Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Taylor, R. K.; Miller, V. L.; Furlong, D. B. Proceedings of the National Academy of Sciences, Vol. 84, Issue 9 https://doi.org/10.1073/pnas.84.9.2833	journal	May 1987
Gut-on-chip: Recreating human intestine in vitro Xiang, Yunqing; Wen, Hui; Yu, Yue Journal of Tissue Engineering, Vol. 11 https://doi.org/10.1177/2041731420965318	journal	January 2020
Cell-Type-Specific High Throughput Toxicity Testing in Human Midbrain Organoids Renner, Henrik; Becker, Katharina J.; Kagermeier, Theresa E. Frontiers in Molecular Neuroscience, Vol. 14 https://doi.org/10.3389/fnmol.2021.715054	journal	July 2021
Microbial high throughput phenomics: The potential of an irreplaceable omics Acin-Albiac, Marta; Filannino, Pasquale; Gobbetti, Marco Computational and Structural Biotechnology Journal, Vol. 18 https://doi.org/10.1016/j.csbj.2020.08.010	journal	January 2020
A novel high-throughput assay to quantify the vaccine-induced inhibition of Bordetella pertussis adhesion to airway epithelia Zanaboni, Elisa; Arato, Vanessa; Pizza, Mariagrazia BMC Microbiology, Vol. 16, Issue 1 https://doi.org/10.1186/s12866-016-0829-x	journal	September 2016
Methods to detect apoptotic cell death Banfalvi, Gaspar Apoptosis, Vol. 22, Issue 2 https://doi.org/10.1007/s10495-016-1333-3	journal	December 2016
Microbial functional diversity: From concepts to applications Escalas, Arthur; Hale, Lauren; Voordeckers, James W. Ecology and Evolution, Vol. 9, Issue 20 https://doi.org/10.1002/ece3.5670	journal	September 2019
Rapid antimicrobial susceptibility test for identification of new therapeutics and drug combinations against multidrug-resistant bacteria Sun, Wei; Weingarten, Rebecca A.; Xu, Miao Emerging Microbes & Infections, Vol. 5, Issue 1 https://doi.org/10.1038/emi.2016.123	journal	January 2016
Adhesion of Human and Animal Escherichia coli Strains in Association with Their Virulence-Associated Genes and Phylogenetic Origins Frömmel, Ulrike; Lehmann, Werner; Rödiger, Stefan Applied and Environmental Microbiology, Vol. 79, Issue 19 https://doi.org/10.1128/AEM.01384-13	journal	October 2013
Ecosystem Traits Linking Functional Traits to Macroecology He, Nianpeng; Liu, Congcong; Piao, Shilong Trends in Ecology & Evolution, Vol. 34, Issue 3 https://doi.org/10.1016/j.tree.2018.11.004	journal	March 2019
Hybrid Resistance and Virulence Plasmids in “High-Risk” Clones of Klebsiella pneumoniae, Including Those Carrying blaNDM-5 Turton, Jane; Davies, Frances; Turton, Jack Microorganisms, Vol. 7, Issue 9 https://doi.org/10.3390/microorganisms7090326	journal	September 2019
Trypan Blue Exclusion Test of Cell Viability Strober, Warren Current Protocols in Immunology, Vol. 111, Issue 1 https://doi.org/10.1002/0471142735.ima03bs111	journal	November 2015
Development of a Robust and Quantitative High-Throughput Screening Method for Antibiotic Production in Bacterial Libraries Murray, Elizabeth M.; Allen, Catherine F.; Handy, Tess E. ACS Omega, Vol. 4, Issue 13 https://doi.org/10.1021/acsomega.9b01461	journal	September 2019
Combined selective culture and molecular methods for the detection of carbapenem-resistant organisms from fecal specimens Yee, Rebecca; Fisher, Stefanie; Bergman, Yehudit European Journal of Clinical Microbiology & Infectious Diseases, Vol. 40, Issue 11 https://doi.org/10.1007/s10096-021-04281-8	journal	June 2021
Genome-wide Analysis of Salmonella enterica serovar Typhi in Humanized Mice Reveals Key Virulence Features Karlinsey, Joyce E.; Stepien, Taylor A.; Mayho, Matthew Cell Host & Microbe, Vol. 26, Issue 3 https://doi.org/10.1016/j.chom.2019.08.001	journal	September 2019
Correction: Experimental evaluation of ecological principles to understand and modulate the outcome of bacterial strain competition in gut microbiomes Segura Munoz, Rafael R.; Mantz, Sara; Martínez, Ines The ISME Journal, Vol. 16, Issue 6 https://doi.org/10.1038/s41396-022-01227-6	journal	April 2022
Coexistence of Antibiotic Resistance Genes and Virulence Factors Deciphered by Large-Scale Complete Genome Analysis Pan, Yu; Zeng, Jiaxiong; Li, Liguan mSystems, Vol. 5, Issue 3 https://doi.org/10.1128/mSystems.00821-19	journal	June 2020
Seeking patterns of antibiotic resistance in ATLAS, an open, raw MIC database with patient metadata Catalán, Pablo; Wood, Emily; Blair, Jessica M. A. Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-30635-7	journal	May 2022
Type III Secretion Systems and Disease Coburn, B.; Sekirov, I.; Finlay, B. B. Clinical Microbiology Reviews, Vol. 20, Issue 4 https://doi.org/10.1128/CMR.00013-07	journal	October 2007
Enteric Escherichia coli O157:H7 in Cattle, and the Use of Mice as a Model to Elucidate Key Aspects of the Host-Pathogen-Microbiota Interaction: A Review Lange, Maximo E.; Uwiera, Richard R. E.; Inglis, G. Douglas Frontiers in Veterinary Science, Vol. 9 https://doi.org/10.3389/fvets.2022.937866	journal	July 2022
Assessing Pseudomonas Virulence Using Host Cells Bucior, Iwona; Tran, Cindy; Engel, Joanne Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-0473-0_57	book	January 2014
Vero response to a cytotoxin of Escherichia coli Konowalchuk, J.; Speirs, J. I.; Stavric, S. Infection and Immunity, Vol. 18, Issue 3 https://doi.org/10.1128/iai.18.3.775-779.1977	journal	December 1977
Modern Tools for Rapid Diagnostics of Antimicrobial Resistance Vasala, Antti; Hytönen, Vesa P.; Laitinen, Olli H. Frontiers in Cellular and Infection Microbiology, Vol. 10 https://doi.org/10.3389/fcimb.2020.00308	journal	July 2020
Value of multiplex PCR for detection of antimicrobial resistance in samples retrieved from patients with orthopaedic infections Sigmund, Irene Katharina; Renz, Nora; Feihl, Susanne BMC Microbiology, Vol. 20, Issue 1 https://doi.org/10.1186/s12866-020-01741-7	journal	April 2020
High-throughput assays of phagocytosis, phagosome maturation, and bacterial invasion Steinberg, Benjamin E.; Scott, Cameron C.; Grinstein, Sergio American Journal of Physiology-Cell Physiology, Vol. 292, Issue 2 https://doi.org/10.1152/ajpcell.00358.2006	journal	February 2007
Experimental evolution and the dynamics of adaptation and genome evolution in microbial populations Lenski, Richard E. The ISME Journal, Vol. 11, Issue 10 https://doi.org/10.1038/ismej.2017.69	journal	May 2017
UniProt: the universal protein knowledgebase in 2021 Bateman, Alex; Martin, Maria-Jesus; Orchard, Sandra Nucleic Acids Research, Vol. 49, Issue D1 https://doi.org/10.1093/nar/gkaa1100	journal	November 2020
Development of flow cytometry based adherence assay for Neisseria gonorrhoeae using 5′-carboxyfluorosceinsuccidyl ester Thakur, Sidharath Dev; Obradovic, Milan; Dillon, Jo-Anne R. BMC Microbiology, Vol. 19, Issue 1 https://doi.org/10.1186/s12866-019-1438-2	journal	March 2019
Development and validation of a high-throughput whole cell assay to investigate Staphylococcus aureus adhesion to host ligands Petrie, Laurenne E.; Leonard, Allison C.; Murphy, Julia Journal of Biological Chemistry, Vol. 295, Issue 49 https://doi.org/10.1074/jbc.RA120.015360	journal	December 2020
Bacterial toxins: Offensive, defensive, or something else altogether? Rudkin, Justine K.; McLoughlin, Rachel M.; Preston, Andrew PLOS Pathogens, Vol. 13, Issue 9 https://doi.org/10.1371/journal.ppat.1006452	journal	September 2017
Developmental roadmap for antimicrobial susceptibility testing systems van Belkum, Alex; Bachmann, Till T.; Lüdke, Gerd Nature Reviews Microbiology, Vol. 17, Issue 1 https://doi.org/10.1038/s41579-018-0098-9	journal	October 2018
Droplet Microfluidics for High-Throughput Analysis of Antibiotic Susceptibility in Bacterial Cells and Populations Postek, Witold; Garstecki, Piotr Accounts of Chemical Research, Vol. 55, Issue 5 https://doi.org/10.1021/acs.accounts.1c00729	journal	February 2022
Trait Evolution, Community Assembly, and the Phylogenetic Structure of Ecological Communities Kraft, Nathan J. B.; Cornwell, William K.; Webb, Campbell O. The American Naturalist, Vol. 170, Issue 2 https://doi.org/10.1086/519400	journal	August 2007
Apoptosis pathway-targeted drugs—from the bench to the clinic Cummings, Jeff; Ward, Tim H.; Ranson, Malcolm Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Vol. 1705, Issue 1 https://doi.org/10.1016/j.bbcan.2004.09.005	journal	December 2004
A decade of advances in transposon-insertion sequencing Cain, Amy K.; Barquist, Lars; Goodman, Andrew L. Nature Reviews Genetics, Vol. 21, Issue 9 https://doi.org/10.1038/s41576-020-0244-x	journal	June 2020
Detection of Methicillin Resistance in Staphylococcus aureus From Agar Cultures and Directly From Positive Blood Cultures Using MALDI-TOF Mass Spectrometry-Based Direct-on-Target Microdroplet Growth Assay Nix, Ilka D.; Idelevich, Evgeny A.; Storck, Luise M. Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.00232	journal	February 2020
Common Themes in the Design and Function of Bacterial Effectors Galán, Jorge E. Cell Host & Microbe, Vol. 5, Issue 6 https://doi.org/10.1016/j.chom.2009.04.008	journal	June 2009
A Synthetic Hydrogel, VitroGel® ORGANOID-3, Improves Immune Cell-Epithelial Interactions in a Tissue Chip Co-Culture Model of Human Gastric Organoids and Dendritic Cells Cherne, Michelle D.; Sidar, Barkan; Sebrell, T. Andrew Frontiers in Pharmacology, Vol. 12 https://doi.org/10.3389/fphar.2021.707891	journal	September 2021
Cell death assays for drug discovery Kepp, Oliver; Galluzzi, Lorenzo; Lipinski, Marta Nature Reviews Drug Discovery, Vol. 10, Issue 3 https://doi.org/10.1038/nrd3373	journal	March 2011
Proteomic Methods of Detection and Quantification of Protein Toxins Duracova, Miloslava; Klimentova, Jana; Fucikova, Alena Toxins, Vol. 10, Issue 3 https://doi.org/10.3390/toxins10030099	journal	February 2018
Control of Bacterial Virulence through the Peptide Signature of the Habitat Krypotou, Emilia; Scortti, Mariela; Grundström, Christin Cell Reports, Vol. 26, Issue 7 https://doi.org/10.1016/j.celrep.2019.01.073	journal	February 2019
Novel One-Step Method for Detection and Isolation of Active-Toxin-Producing Clostridium difficile Strains Directly from Stool Samples Darkoh, Charles; DuPont, Herbert L.; Kaplan, Heidi B. Journal of Clinical Microbiology, Vol. 49, Issue 12 https://doi.org/10.1128/JCM.01033-11	journal	December 2011
A high-throughput cell culture system based on capillary and centrifugal actions for rapid antimicrobial susceptibility testing Lim, Taegeun; Kim, Eun-Geun; Choi, Jungil Lab on a Chip, Vol. 20, Issue 24 https://doi.org/10.1039/d0lc00753f	journal	January 2020
Trait-based approaches for understanding microbial biodiversity and ecosystem functioning Krause, Sascha; Le Roux, Xavier; Niklaus, Pascal A. Frontiers in Microbiology, Vol. 5 https://doi.org/10.3389/fmicb.2014.00251	journal	May 2014
Environmental and Physiological Factors Affecting High-Throughput Measurements of Bacterial Growth Atolia, Esha; Cesar, Spencer; Arjes, Heidi A. mBio, Vol. 11, Issue 5 https://doi.org/10.1128/mBio.01378-20	journal	October 2020
Expanding the Pathogenic Potential Concept To Incorporate Fulminancy, Time, and Virulence Factors Casadevall, Arturo mSphere, Vol. 7, Issue 1 https://doi.org/10.1128/msphere.01021-21	journal	February 2022
Role of Escherichia coli O157:H7 Virulence Factors in Colonization at the Bovine Terminal Rectal Mucosa Sheng, H.; Lim, J. Y.; Knecht, H. J. Infection and Immunity, Vol. 74, Issue 8 https://doi.org/10.1128/IAI.00406-06	journal	July 2006
Engineered Cell Line Imaging Assay Differentiates Pathogenic from Non-Pathogenic Bacteria Phillips, Shelby M. B.; Bergstrom, Carson; Walker, Brian Pathogens, Vol. 11, Issue 2 https://doi.org/10.3390/pathogens11020209	journal	February 2022
Bacterial Adhesion and Entry into Host Cells Pizarro-Cerdá, Javier; Cossart, Pascale Cell, Vol. 124, Issue 4 https://doi.org/10.1016/j.cell.2006.02.012	journal	February 2006
Growth rate, transmission mode and virulence in human pathogens Leggett, Helen C.; Cornwallis, Charlie K.; Buckling, Angus Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 372, Issue 1719 https://doi.org/10.1098/rstb.2016.0094	journal	March 2017
RFP-based method for real-time tracking of invasive bacteria in a heterogeneous population of cells Akinsola, Rasaq Olajide; Adewoyin, Malik; Lee, Choon-Weng Analytical Biochemistry, Vol. 634 https://doi.org/10.1016/j.ab.2021.114432	journal	December 2021
Emerging Patterns of Microbial Functional Traits Yang, Yunfeng Trends in Microbiology, Vol. 29, Issue 10 https://doi.org/10.1016/j.tim.2021.04.004	journal	October 2021
A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA Hooker, David J.; Mobarok, Masqura; Anderson, Jenny L. Nucleic Acids Research, Vol. 40, Issue 15 https://doi.org/10.1093/nar/gks334	journal	April 2012
Phenotype-Based Threat Assessment Yang, Jing; Eslami, Mohammed; Chen, Yi-Pei Proceedings of the National Academy of Sciences, Vol. 119, Issue 14 https://doi.org/10.1073/pnas.2112886119	journal	April 2022
Novel genes associated with enhanced motility of Escherichia coli ST131 Kakkanat, Asha; Phan, Minh-Duy; Lo, Alvin W. PLOS ONE, Vol. 12, Issue 5 https://doi.org/10.1371/journal.pone.0176290	journal	May 2017
Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes Watterson, William J.; Tanyeri, Melikhan; Watson, Andrea R. eLife, Vol. 9 https://doi.org/10.7554/eLife.56998	journal	June 2020
Oncogene-induced senescence and tumour control in complex biological systems Galluzzi, Lorenzo; Vitale, Ilio Cell Death & Differentiation, Vol. 25, Issue 6 https://doi.org/10.1038/s41418-018-0102-y	journal	April 2018
Direct-on-Target Microdroplet Growth Assay Applications for Clinical Antimicrobial Susceptibility Testing Li, Rongrong; Tang, Hao; Xu, Huaming Infection and Drug Resistance, Vol. Volume 14 https://doi.org/10.2147/IDR.S303187	journal	April 2021
Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods Gajic, Ina; Kabic, Jovana; Kekic, Dusan Antibiotics, Vol. 11, Issue 4 https://doi.org/10.3390/antibiotics11040427	journal	March 2022
Big Data in Chemical Toxicity Research: The Use of High-Throughput Screening Assays To Identify Potential Toxicants Zhu, Hao; Zhang, Jun; Kim, Marlene T. Chemical Research in Toxicology, Vol. 27, Issue 10 https://doi.org/10.1021/tx500145h	journal	September 2014
Beyond the List: Bioagent-Agnostic Signatures Could Enable a More Flexible and Resilient Biodefense Posture Than an Approach Based on Priority Agent Lists Alone Leiser, Owen P.; Hobbs, Errett C.; Sims, Amy C. Pathogens, Vol. 10, Issue 11 https://doi.org/10.3390/pathogens10111497	journal	November 2021
Using Machine Learning To Predict Antimicrobial MICs and Associated Genomic Features for Nontyphoidal Salmonella Nguyen, Marcus; Long, S. Wesley; McDermott, Patrick F. Journal of Clinical Microbiology, Vol. 57, Issue 2 https://doi.org/10.1128/JCM.01260-18	journal	October 2018
Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies Sunde, Marianne; Ramstad, Silje N.; Rudi, Knut Journal of Global Antimicrobial Resistance, Vol. 26 https://doi.org/10.1016/j.jgar.2021.06.003	journal	September 2021
A Type III Secretion Negative Clinical Strain of Pseudomonas aeruginosa Employs a Two-Partner Secreted Exolysin to Induce Hemorrhagic Pneumonia Elsen, Sylvie; Huber, Philippe; Bouillot, Stéphanie Cell Host & Microbe, Vol. 15, Issue 2 https://doi.org/10.1016/j.chom.2014.01.003	journal	February 2014
A global genomic approach uncovers novel components for twitching motility-mediated biofilm expansion in Pseudomonas aeruginosa Nolan, Laura M.; Whitchurch, Cynthia B.; Barquist, Lars Microbial Genomics, Vol. 4, Issue 11 https://doi.org/10.1099/mgen.0.000229	journal	November 2018
Let the concept of trait be functional! Violle, Cyrille; Navas, Marie-Laure; Vile, Denis Oikos, Vol. 116, Issue 5 https://doi.org/10.1111/j.0030-1299.2007.15559.x	journal	May 2007
Organ‐on‐chip to investigate host‐pathogens interactions Feaugas, Thomas; Sauvonnet, Nathalie Cellular Microbiology, Vol. 23, Issue 7 https://doi.org/10.1111/cmi.13336	journal	April 2021
Genes Involved in Cronobacter sakazakii Biofilm Formation Hartmann, Isabel; Carranza, Paula; Lehner, Angelika Applied and Environmental Microbiology, Vol. 76, Issue 7 https://doi.org/10.1128/AEM.00930-09	journal	April 2010
Secreted Autotransporter Toxin (Sat) Mediates Innate Immune System Evasion Freire, Claudia A.; Silva, Rosa M.; Ruiz, Rita C. Frontiers in Immunology, Vol. 13 https://doi.org/10.3389/fimmu.2022.844878	journal	February 2022
Bacterial fitness landscapes stratify based on proteome allocation associated with discrete aero-types Chen, Ke; Anand, Amitesh; Olson, Connor PLOS Computational Biology, Vol. 17, Issue 1 https://doi.org/10.1371/journal.pcbi.1008596	journal	January 2021
Die another way – non-apoptotic mechanisms of cell death Tait, Stephen W. G.; Ichim, Gabriel; Green, Douglas R. Journal of Cell Science, Vol. 127, Issue 10 https://doi.org/10.1242/jcs.093575	journal	May 2014
Antibiotic resistance in microbes: History, mechanisms, therapeutic strategies and future prospects Uddin, Tanvir Mahtab; Chakraborty, Arka Jyoti; Khusro, Ameer Journal of Infection and Public Health, Vol. 14, Issue 12 https://doi.org/10.1016/j.jiph.2021.10.020	journal	December 2021
Fitness ofEscherichia colimutants with reduced susceptibility to tigecycline Linkevicius, Marius; Anderssen, Jytte Mark; Sandegren, Linus Journal of Antimicrobial Chemotherapy, Vol. 71, Issue 5 https://doi.org/10.1093/jac/dkv486	journal	February 2016
Computational approach to predict species-specific type III secretion system (T3SS) effectors using single and multiple genomes Hobbs, Christopher K.; Porter, Vanessa L.; Stow, Maxwell L. S. BMC Genomics, Vol. 17, Issue 1 https://doi.org/10.1186/s12864-016-3363-1	journal	December 2016
High-Throughput Quantification of Bacterial-Cell Interactions Using Virtual Colony Counts Hoffmann, Stefanie; Walter, Steffi; Blume, Anne-Kathrin Frontiers in Cellular and Infection Microbiology, Vol. 8 https://doi.org/10.3389/fcimb.2018.00043	journal	February 2018
Transfer of antimicrobial resistance plasmids from Klebsiella pneumoniae to Escherichia coli in the mouse intestine Schjorring, S.; Struve, C.; Krogfelt, K. A. Journal of Antimicrobial Chemotherapy, Vol. 62, Issue 5 https://doi.org/10.1093/jac/dkn323	journal	July 2008
A method for high throughput determination of viable bacteria cell counts in 96-well plates Hazan, Ronen; Que, Yok-Ai; Maura, Damien BMC Microbiology, Vol. 12, Issue 1 https://doi.org/10.1186/1471-2180-12-259	journal	November 2012

Similar Records

Metabolomics Analysis of Bacterial Pathogen Burkholderia thailandensis and Mammalian Host Cells in Co-culture

Journal Article · 2022 · ACS Infectious Diseases · OSTI ID:1877157

Aiosa, Nicole; Sinha, Anupama; Jaiyesimi, Olakunle A.; +3 more

Bacterial diversity analysis of Huanglongbing pathogen-infected citrus, using PhyloChip and 16S rRNA gene clone library sequencing

Journal Article · 2009 · Applied and Environmental Microbiology · OSTI ID:950108

Shankar Sagaram, U; DeAngelis, K M; Trivedi, P; +3 more

Distinctiveness of genes contributing to growth of Pseudomonas syringae in diverse host plant species

Journal Article · 2020 · PLoS ONE · OSTI ID:1816085

Helmann, Tyler C.; Deutschbauer, Adam M.; Lindow, Steven E.

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
bacterial pathogen
high-throughput methods
microbial functional traits
pathogen agnostic detection
pathogenesis

Title: High-throughput functional trait testing for bacterial pathogens

Citation Formats

References (84)

Similar Records

Related Subjects